Liquid Chromatography–Tandem Mass Spectrometry Analysis of Aminoglycosides in Foods Using an Ethylene-Bridged Hybrid Zwitterionic Stationary Phase and Hydrophilic–Lipophilic-Balanced Solid-Phase Extraction Cartridges

This work aimed to develop an analytical method for the screening of multiple aminoglycoside residues in foods of animal origin using an ethylene-bridged hybrid (BEH) particle-based sulfoalkylbetaine stationary phase. The effects of chromatographic conditions on the separation of 17 aminoglycosides have been systematically investigated. Sample preparation and mass spectrometry detection have also been investigated and optimized. In contrast to high buffer concentrations in the mobile phase required for silica-based sulfoalkylbetaine stationary phases, a moderate buffer concentration (20 mM) provided the optimal separation of 17 aminoglycosides with the BEH sulfoalkylbetaine stationary phase. The developed method has been evaluated in milk, beef, pork, liver, and honey samples with good performance for retention, selectivity, sensitivity, linearity, precision, and accuracy. The majority of the limit of quantitation estimated with the matrix was less than 25 μg/kg. The overall accuracy across five matrices was in the range from 96 to 111%, with standard deviations of less than 19%

sj-pdf-2-ijd-10.1177_10567895211056679 - Supplemental material for Short beam shear damage analysis of GLARE laminates based on digital image correlation and finite element analysis

Supplemental material, sj-pdf-2-ijd-10.1177_10567895211056679 for Short beam shear damage analysis of GLARE laminates based on digital image correlation and finite element analysis by Yajun Chen, Jinchuan Yang, Fusheng Wang and Jianshu Peng in International Journal of Damage Mechanics</p

sj-pdf-1-ijd-10.1177_10567895211056679 - Supplemental material for Short beam shear damage analysis of GLARE laminates based on digital image correlation and finite element analysis

Supplemental material, sj-pdf-1-ijd-10.1177_10567895211056679 for Short beam shear damage analysis of GLARE laminates based on digital image correlation and finite element analysis by Yajun Chen, Jinchuan Yang, Fusheng Wang and Jianshu Peng in International Journal of Damage Mechanics</p

sj-pdf-3-ijd-10.1177_10567895211056679 - Supplemental material for Short beam shear damage analysis of GLARE laminates based on digital image correlation and finite element analysis

Supplemental material, sj-pdf-3-ijd-10.1177_10567895211056679 for Short beam shear damage analysis of GLARE laminates based on digital image correlation and finite element analysis by Yajun Chen, Jinchuan Yang, Fusheng Wang and Jianshu Peng in International Journal of Damage Mechanics</p

Preparation of Aggregation Stable Gold Nanoparticles Using Star-Block Copolymers

Nanoparticles of improved stability against long-term aggregation were prepared using poly(styrene)-b-poly(2-vinylpyridine) (PS-b-P2VP) star-block copolymer architectures. The star-block copolymers, physically resembling diblock copolymer micelles, were synthesized by anionic polymerization and coupling with ethylene glycol dimethacrylate. They contain P2VP core segments that facilitated conversion of HAuCl4 to single gold nanoparticles. The size distribution and long-term stability against aggregation of the gold nanoparticles were investigated both as solution and as films. UV−vis spectroscopy and transmission electron microscope images reveal long-term stability against aggregation up to 1 month

Additional file 2 of Anaplasma phagocytophilum in Marmota himalayana

Additional file 2: Figure S2. Collinearity between marmot-derived A. phagocytophilum (JAHLEX000000000) and 13 worldwide strains

Additional file 1 of Anaplasma phagocytophilum in Marmota himalayana

Additional file 1: Figure S1. Sequencing depth and G+C content of the whole-genome sequenced marmot-derived A. phagocytophilum (JAHLEX000000000)

Additional file 3 of Anaplasma phagocytophilum in Marmota himalayana

Additional file 3: Table S1. Characteristics of samples positive for A. phagocytophilum in M. himalayana. Grey column: samples screened for A. phagocytophilum. √: Positive samples for A. phagocytophilum, confirmed by both 16s rRNA and groESL gene sequences. *: Positive samples had a 1380-bp cloned sequence of groESL. a: Y, marmots found dead in the environment. N, marmots captured for plague surveillance

Additional file 4 of Anaplasma phagocytophilum in Marmota himalayana

Additional file 4: Table S2. Nucleotide sequence identity matrix for groEL genes within the Anaplasma genus

Additional file 5 of Anaplasma phagocytophilum in Marmota himalayana

Additional file 5: Table S3. Characteristics of 13 A. phagocytophilum strains compared in this study