Additional file 1: of Comparison of sodium ion levels between an arterial blood gas analyzer and an autoanalyzer in preterm infants admitted to the neonatal intensive care unit: a retrospective study

The datasets supporting the conclusion of this study. (XLSX 820 kb

Synthesis and Reactivity of Nickel(II) Hydroxycarbonyl Species, NiCOOH‑κ<i>C</i>

Reactions of nickel complexes supported by an anionic PNP pincer ligand (PNP^– = N­[2-P^<i>i</i>Pr₂-4-Me-C₆H₃]₂) toward CO₂ and CO are investigated, particularly for interrogating their C–O bond formation/cleavage chemistry. The formation of a nickel formate species (<b>2</b>) was accomplished by the reaction of (PNP)­NiH with CO₂, while the structural isomer complex (PNP)­NiCOOH-κ<i>C</i> (<b>4</b>) was successfully produced from the corresponding nickel hydroxyl compound by exposing it to CO­(g). Its structurally unique character was gleaned by obtaining two solid-state structures for (PNP)­NiCOOH-κ<i>C</i> (<b>4</b>) and {(PNP)­Ni}₂-μ-CO₂-κ²<i>C</i>,<i>O</i> (<b>6</b>); the latter was obtained from the reaction of <b>4</b> with a nickel hydroxyl complex. Both species possess a NiCOO-κ<i>C</i> binding mode, which is reminiscent of the binding mode found at the carbon monoxide dehydrogenase (CODH) active site with its Ni–COO–Fe fragment. The cationic species {(PNP)­NiCO}⁺ (<b>7</b>) was also prepared via the protonation of <b>4</b>, which then led to the investigation of the C–O bond formation in <b>7</b> by adding a nucleophile such as OH^–

BMP-2 Promotes Oral Squamous Carcinoma Cell Invasion by Inducing CCL5 Release

<div><p>Bone morphogenetic protein-2 (BMP-2)-containing bone grafts are useful regenerative materials for oral and maxillofacial surgery; however, several <i>in</i><i>vitro</i> and <i>in</i><i>vivo</i> studies previously reported cancer progression-related adverse effects caused by BMP-2. In this study, by quantifying the rhBMP-2 content released from bone grafts, the rhBMP-2 concentration that did not show cytotoxicity in each cell line was determined and applied to the <i>in</i><i>vitro</i> monoculture or coculture model in the invasion assay. Our results showed that 1 ng/ml rhBMP-2, while not affecting cancer cell viability, significantly increased the invasion ability of the cancer cells cocultured with fibroblasts. Cocultured medium with rhBMP-2 also contained increased levels of matrix metalloproteinases. rhBMP-2-treated cocultured fibroblasts did not show a prominent difference in mRNA expression profile. Some cytokines, however, were detected in the conditioned medium by a human cytokine antibody array. Among them, the cancer invasion-related factor CCL5 was quantified by ELISA. Interestingly, CCL5 neutralizing antibodies significantly reduced the invasion of oral cancer cells. In conclusion, our results suggest that 1 ng/ml rhBMP-2 may induce invasion of oral squamous cell carcinoma (OSCC) cells by CCL5 release in coculture models. Therefore, we propose that a careful clinical examination before the use of rhBMP-2-containing biomaterials is indispensable for using rhBMP-2 treatment to prevent cancer progression.</p></div

MOESM1 of Molecular generative model based on conditional variational autoencoder for de novo molecular design

Supplementary material 1 (docx 791 KB

Invasion assay in monoculture or coculture models.

<p>(a) Schematic diagram of the invasion assay and cellular changes. Carcinoma cells on the transwell penetrate the collagen membrane when cocultured with fibroblasts and then finally attach to the lower surface of the transwell. (b–g) Cocultured YD-10B (b and c), YD-38 (d and e), and HSC-2 (f and g) cells either untreated (b, d, and f) or treated with 1 ng/ml rhBMP-2 (c, e, and g). (h) Quantification of the invasion ability of OSCC cells. Data shown are means of three independent repeats, and error bars indicate standard deviation. *P<0.05 compared to the control.</p

MOESM1 of Preliminary paleomagnetic and rock magnetic results from 17 to 22Â ka sediment of Jeju Island, Korea: Geomagnetic excursional behavior or rock magnetic anomalies?

Additional file 1. This includes a figure comparing paleomagnetic inclination data for East Asia from some previous literatures and this study (Figure S1), available AMS 14C age data of the Gosan Formation (Table S1), some rock magnetic properties data (Table S2), AF-derived paleomagnetic direction data (Table S3), TH-derived paleomagnetic direction data (Table S4), and the estimated age-depth model data (Table S5) for the GSDS site of the Gosan Formation

MMP and cytokine antibody array for cocultured YD-38 and fibroblasts.

<p>(a–c) MMP array for cocultured YD-38 cells either untreated (a) or treated with rhBMP-2 (b). (c) Quantification of the levels of various MMPs. (d–f) Cytokine array of cocultured YD-38 cells either untreated (d) or treated with rhBMP-2 (e). (f) Quantification of the levels of various cytokines. Red boxes in (d) and (e) show the changes of signal intensities of CCL5 before and after 1 ng/ml rhBMP-2 treatment, respectively, which were quantified and compared in the red box of (f).</p

Invasion assay of coculture models treated with rhBMP-2 and CCL5 neutralizing antibodies.

<p>(a–f) Representative invasion results of YD-10B (a and b), YD-38 (c and d), and HSC-2 (e and f) cocultured cells treated with either 1 ng/ml rhBMP-2 only (a, c, and e) or both rhBMP-2 and 0.05 µg/ml CCL5 neutralizing antibodies (b, d, and f). (g) Quantification of the invasion ability of OSCC cell lines. Data shown are means of three independent repeats, and error bars indicate standard deviation. *P<0.05 compared to the control.</p

Human CCL5 ELISA of monocultured or cocultured medium with or without 1 ng/ml rhBMP-2.

<p>Data shown are means of three independent repeats, and error bars indicate standard deviation. *P<0.05 compared to the control.</p

Primer sequences used for RT-PCR and Real-time PCR.

<p>Primer sequences used for RT-PCR and Real-time PCR.</p