Additional file 3 of Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Additional file 3: Differentially expressed miRNAs in mock-infected and DHAV-3-infected duckling liver

Additional file 9 of Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Additional file 9: GO enrichment analysis of DEGs in mock-infected and DHAV-3-infected duckling liver

Additional file 7 of Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Additional file 7: Overview of mRNA-Seq in the mock-infected and DHAV-3-infected libraries

Additional file 14 of Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Additional file 14: The primers used in this study

Additional file 11 of Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Additional file 11: KEGG pathway analysis of DEGs in mock- and DHAV-3-infected duckling livers

Optimization of fermentation conditions for production of neutral metalloprotease by <i>Bacillus subtilis</i> SCK6 and its application in goatskin-dehairing

In this study, a high protease-producing strain was screened by spread plate method and identified by molecular biology and morphological identification. It was identified as Bacillus sp. LCB14. A neutral protease gene was cloned and heterologous expressed by B. subtilis SCK6. Then, the recombinant protease was used to dehair the goat skins. The fermentation conditions of neutral protease production by B. subtilis SCK6 were optimized. The single factor experiments, Plackett–Burma experiment, and response surface method were conducted to determine fermentation medium and culture conditions. The optimized medium contained corn meal 49 g/L, soluble starch 28 g/L, soybean meal 17 g/L, corn steep liquor powder 8 g/L, yeast extract 10 g/L, Na2HPO4 2.3 g/L, KH2PO4 1.9 g/L, MgSO4 0.5 g/L, MnCl2 0.1 g/L and ZnSO4 0.05 g/L. The optimized culture conditions were 35 °C and pH 7.0. Under the optimum conditions, the recombinant strain reached 33467.28 U/mL after 72 hr ferment. Moreover, by fed batch in 30 L fermenters, neutral protease production reached 39,440.78 U/mL and shortened fermentation time from 72 hr to 46 hr. Finally, the crude enzyme was utilized to replace sodium sulfide for dehairing of goatskins. The enzymatic dehaired pelts were white, smooth, and soft; the grain side of enzymatic dehaired pelts were clear; there was no obvious damage to the grain side of enzymatic dehaired pelts by visual observation and tactile test. Furthermore, there were no hair roots, hair follicles and other glands in enzymatic dehaired belts, and the collagen fibers of enzymatic dehaired belt were dispersed well by histological analysis.</p

Additional file 10 of Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Additional file 10: GO analysis information of DEGs significantly enriched in 244 biological processes

Additional file 12 of Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Additional file 12: The top 20 enriched KEGG pathways of DEGs

Additional file 1 of Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Additional file 1: Known miRNAs identified in this study

Additional file 4 of Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Additional file 4: GO annotation of the predicted target genes of all miRNAs in this study