DataSheet1_UroVysion™ fluorescence in situ hybridization (FISH) possibly has a high positive rate in carcinoma of non-urothelial lineages.PDF

Background: Positive UroVysion™ fluorescence in situ hybridization (FISH) is generally considered as urothelial carcinoma (UC). We clarify if UroVysion™ FISH can be positive in carcinoma of non-urothelial lineages (CNUL), and verify the consistency of urine FISH and histological FISH in CNUL.Methods: All CNUL subjects detected by urine FISH assay due to haematuria from Tongji Hospital were screened. Meanwhile, 2 glandular cystitis and 2 urothelial carcinoma were served as negative or positive control. Paraffin-embedded tissue sections of all subjects were sent to the pathology department for histological FISH detection.Results: A total of 27 patients were included in this study, including 9 with adenocarcinomas, 11 with squamous cell carcinomas, and 7 with other tumour types. The overall positive rate in urine FISH was 64.00% (16/25) in patients with CNUL, 77.78% (7/9) in those with adenocarcinoma and 54.55% (6/11) in those with squamous carcinoma. There was a significant difference in the GLP p16 gene deletion rate between UC and CNUL (100% vs. 8.00%, p = 0.017). Histological FISH results showed that the histological results of 19 patients were consistent with their urine FISH results, and only one patient with stage Ⅲa urachal carcinoma had inconsistent histological FISH results (positive) and urine FISH (negative) results.Conclusion: We demonstrated for the first time the application value of FISH in CNUL on urine samples. Positive urine FISH tests indicate not only UC, but also CNUL. UroVysion™ FISH possibly has a high positive rate in CNUL. CNUL and UC have different genetic changes shown by FISH.</p

Synergistic Effect of ZSM‑5 Zeolite in Pt–CeO₂–TiO₂/ZSM‑5 Catalysts for Highly Efficient Catalytic Oxidation of VOCs

A series of Pt–CeTi/ZSM-5 catalysts (0.5 wt % Pt) with different CeO2–TiO2/ZSM-5 mass ratios were synthesized and used for the catalytic oxidation of 1,2-dichloroethane and benzene. The results reveal that the strong Pt/CeO2–TiO2 interaction can be evidently enhanced by introducing proper amounts of CeO2–TiO2 mixed oxides, resulting in the improvement of Pt dispersion and redox properties as well as the production of more defective oxygen and Pt2+ species. The synergistic effect between abundant ZSM-5 acid sites (especially strong acid sites) and strong Pt/CeO2–TiO2 oxidizing sites facilitates the deep oxidation of benzene, intermediates, and byproducts generated from the 1,2-dichloroethane oxidation process at lower temperature. Among the catalysts, Pt–CeTi/ZSM-5­(1/10) with the CeO2–TiO2/ZSM-5 mass ratio of 1/10 exhibits the greatest activity for benzene and 1,2-dichloroethane oxidation, showing the T90% of 145 and 245 °C, respectively. Moreover, the catalyst also possesses satisfactory adaptability for the oxidation of various VOCs and durability for 1,2-dichloroethane oxidation

Correction to “Fullerene Nanorings as Nitric Oxide Radical Scavengers for Ultraviolet-Induced Cellular Injury”

Correction to “Fullerene Nanorings as Nitric Oxide Radical Scavengers for Ultraviolet-Induced Cellular Injury

Fullerene Nanorings as Nitric Oxide Radical Scavengers for Ultraviolet-Induced Cellular Injury

Nitric oxide radical (NO•) induced by UV irradiation would exacerbate cellular damage and apoptosis. Though fullerenes are known as excellent radical scavengers, severe aggregation and poor bioavailability often decrease their antioxidant capability in real bioapplications. Herein, a simple but effective method is introduced for the synthesis of a novel hollow fullerene nanostructure (fullerene nanoring, FNR). C60 aggregations produced in m-xylene/isopropyl alcohol (IPA) binary solvents by liquid–liquid interface precipitation strategy are chemically etched by the addition of ethylenediamine (EDA), while aminofullerenes subsequently nucleate at the interface of short-lived EDA-IPA droplets. Careful control of the nucleation kinetics via fine-tuning of the xylene/IPA ratio proved critical for the successful formation of ring-shaped FNR. Such hydrophilic and low-cytotoxic nanostructures possess surprisingly outstanding scavenging performance toward NO• (IC50 = 80 μg/mL). Prominent cytoprotection of FNR against UV-induced DNA oxidation and cellular injury is further confirmed by laser confocal microscopy and flow cytometry. Our results may benefit the upgradation of nanocarbon materials for bioapplications

Ultrasensitive Detection for Lithium-Ion Battery Electrolyte Leakage by Rare-Earth Nd-Doped SnO₂ Nanofibers

The problems of lithium-ion battery (LIB) failure have attracted growing attention since flammable and explosive electrolyte leakage might lead to serious consequences. However, due to the redox-neutral and volatile nature of main electrolyte components, such as dimethyl carbonate (DMC), trace leakages are difficult to detect. Therefore, research on LIB electrolyte sensors is urgent and lacking. Herein, sensors based on rare-earth Nd-doped SnO2 nanofibers are reported for detecting DMC vapor in LIB. The excellent sensitivity (distinct response to 20 ppb DMC), high response (∼38.13–50 ppm DMC), and superior selectivity and stability of 3%Nd-SnO2 suggest that it should be a promising candidate for LIB safety monitors. Meanwhile, it also shows clear and rapid response during the LIB-leakage real-time detection experiment. The doping of Nd endows SnO2 with more oxygen vacancy defects. In addition, the highly active Nd sites greatly enhanced the adsorption energy of DMC on SnO2. All of these features contribute to the improvement of DMC-sensing performances

Image1_To investigate the correlation between normal fetal biventricular myocardial function and gestational age using velocity vector imaging.jpeg

ObjectiveThe aim of this study was to evaluate the left and right ventricular segmental and global myocardial function of normal fetuses using velocity vector imaging and explore the correlation between global myocardial function parameters and gestational age.MethodsA total of 127 normal fetuses were selected and divided into five groups according to gestational age for the measurement of their left and right ventricular segmental and global velocity, strain, and strain rate. This study also explored the change trend in the global myocardial function parameters at different gestational ages and analyzed its correlation with gestational age.ResultsThe peak velocities of the biventricular segments of the normal fetuses showed a decreasing trend from the basal to the middle to the apex segment, and the differences were statistically significant (P  0.05). The peak global velocity of both ventricles increased with the gestational age, and it was moderately correlated with gestational age; however, the correlation of strain and peak strain rate with gestational age was not statistically significant (P > 0.05).ConclusionIn normal fetuses, the peak myocardial velocity of the biventricular segments showed a decreasing trend from the basal to the apical segment. The global peak myocardial velocity was linearly correlated with gestational age; however, the global strain and peak strain rate did not change as gestational age increased, indicating that the myocardial deformability of the fetus' ventricles was constant in the middle and late trimesters.</p

Additional file 2 of KCNJ14 knockdown significantly inhibited the proliferation and migration of colorectal cells

Additional file 2: Fig. S2 Association between KCNJ14 expression and survival of patients at different stages of CRC. (a) Overall survival of stages I–III CRC patients with high or low expression of KCNJ14. (b) Overall survival of stage IV CRC patients with high or low expression of KCNJ14

Dry Reforming of Ethane and Butane with CO₂ over PtNi/CeO₂ Bimetallic Catalysts

Dry reforming is a potential process to convert CO₂ and light alkanes into syngas (H₂ and CO), which can be subsequently transformed to chemicals and fuels. In this work, PtNi bimetallic catalysts have been investigated for dry reforming of ethane and butane using both model surfaces and supported powder catalysts. The PtNi bimetallic catalyst shows an improvement in both activity and stability in comparison to the corresponding monometallic catalysts. The formation of PtNi alloy and the partial reduction of Ce⁴⁺ to Ce³⁺ under reaction conditions are demonstrated by in situ ambient-pressure X-ray photoemission spectroscopy (AP-XPS), X-ray diffraction (XRD), and X-ray absorption fine structure (XAFS) measurements. A Pt-rich bimetallic surface is revealed by diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) following CO adsorption. Combined in situ experimental results and density functional theory (DFT) calculations suggest that the Pt-rich PtNi bimetallic surface structure would weaken the binding of surface oxygenate/carbon species and reduce the activation energy for C–C bond scission, leading to an enhanced dry reforming activity

Additional file 1 of KCNJ14 knockdown significantly inhibited the proliferation and migration of colorectal cells

Additional file 1: Fig. S1 The methylation regulation of KCNJ14 expression. (a) The methylation status of 11 CpG sites of KCNJ14 in colorectal cancer tissue samples based on the TCGA database. (b) The relationship between cg17660703 methylation and KCNJ14 expression. (c) The overall survival of CRC patients with high or low methylation status of cg17660703

Extraordinary Antiwear Properties of Graphene-Reinforced Ti Composites Induced by Interfacial Decoration

The expected excellent lubricant effect of graphene in metals during friction and wear is rarely achieved because of the difficulty in synthesizing suitable interfaces. Particularly, the situation is more challenging in titanium (Ti) matrix composites (TMCs) because of the high chemical-interface-reaction tendency between graphene and Ti during composite fabrication. In this study, few-layered graphene (FLG) decorated with SiC nanoparticles (SiCp) was synthesized as reinforcement in Ti–6Al–4V alloy to improve the interface of the composites. It was found that interfacial SiCp not only strengthened the interface bonding by the Si solid solution but also inhibited the chemical reaction between FLG and the Ti matrix with reduced sp3 defects. The composite with 30 wt % SiC-decorated FLG showed an 86.8% decrement in wear rate compared to the unreinforced matrix, resulting in exceptionally high antiwear enhancing efficiency, which was around fourfold of the available values of other TMCs in the literature. The antiwear mechanism was investigated by thorough characterization of the interfaces and microstructures of the composites. The idea of interfacial decoration can be potentially applied to other nanocarbon/metal composites with the advantages of retaining the function performance of nanocarbon materials