Additional file 1 of Effect of Baihu and Guizhi decoction in acute gouty arthritis: study protocol for a randomized controlled trial

Additional file 1: SPIRIT 2013 checklist. Recommended items to address in a clinical trial protocol and related documents

Additional file 2 of Effect of Baihu and Guizhi decoction in acute gouty arthritis: study protocol for a randomized controlled trial

Additional file 2: Model informed consent for

Image1_The diagnosis and treatment for a patient with cancer of unknown primary: A case report.JPEG

Background: Cancer of unknown primary (CUP) is a class of metastatic malignant tumors whose primary location cannot be determined. The diagnosis and treatment of CUP are a considerable challenge for clinicians. Herein, we report a CUP case whose corresponding primary tumor sites were successfully identified, and the patient received proper treatment.Case report: In February 2022, a 74-year-old woman was admitted to the Medical Oncology Department at Sir Run Run Shaw Hospital for new lung and intestinal tumors after more than 9 years of breast cancer surgery. After laparoscopically assisted right hemicolectomy, pathology revealed mucinous adenocarcinoma; the pathological stage was pT2N0M0. Results from needle biopsies of lung masses suggested poorly differentiated cancer, ER (-), PR (-), and HER2 (-), which combined with the clinical history, did not rule out metastatic breast cancer. A surgical pathology sample was needed to determine the origin of the tumor tissue, but the patient’s chest structure showed no indications for surgery. Analysis of the tumor’s traceable gene expression profile prompted breast cancer, and analysis of next-generation amplification sequencing (NGS) did not obtain a potential drug target. We developed a treatment plan based on comprehensive immunohistochemistry, a gene expression profile, and NGS analysis. The treatment plan was formulated using paclitaxel albumin and capecitabine in combination with radiotherapy. The efficacy evaluation was the partial response (PR) after four cycles of chemotherapy and two cycles combined with radiotherapy.Conclusion: This case highlighted the importance of identifying accurate primary tumor location for patients to benefit from treatment, which will provide a reference for the treatment decisions of CUP tumors in the future.</p

Observation of Moiré Patterns in Twisted Stacks of Bilayer Perovskite Oxide Nanomembranes with Various Lattice Symmetries

The design and fabrication of novel quantum devices in which exotic phenomena arise from moiré physics have sparked a new race of conceptualization and creation of artificial lattice structures. This interest is further extended to the research on thin-film transition metal oxides, with the goal of synthesizing twisted layers of perovskite oxides concurrently revealing moiré landscapes. By utilizing a sacrificial-layer-based approach, we show that such high-quality twisted bilayer oxide nanomembrane structures can be achieved. We observe atomic-scale distinct moiré patterns directly formed with different twist angles, and the symmetry-inequivalent nanomembranes can be stacked together to constitute new complex moiré configurations. This study paves the way to the construction of higher-order artificial oxide heterostructures based on different materials/symmetries and provides the materials foundation for investigating moiré-related electronic effects in an expanded selection of twisted oxide thin films

Table1_The diagnosis and treatment for a patient with cancer of unknown primary: A case report.DOCX

Background: Cancer of unknown primary (CUP) is a class of metastatic malignant tumors whose primary location cannot be determined. The diagnosis and treatment of CUP are a considerable challenge for clinicians. Herein, we report a CUP case whose corresponding primary tumor sites were successfully identified, and the patient received proper treatment.Case report: In February 2022, a 74-year-old woman was admitted to the Medical Oncology Department at Sir Run Run Shaw Hospital for new lung and intestinal tumors after more than 9 years of breast cancer surgery. After laparoscopically assisted right hemicolectomy, pathology revealed mucinous adenocarcinoma; the pathological stage was pT2N0M0. Results from needle biopsies of lung masses suggested poorly differentiated cancer, ER (-), PR (-), and HER2 (-), which combined with the clinical history, did not rule out metastatic breast cancer. A surgical pathology sample was needed to determine the origin of the tumor tissue, but the patient’s chest structure showed no indications for surgery. Analysis of the tumor’s traceable gene expression profile prompted breast cancer, and analysis of next-generation amplification sequencing (NGS) did not obtain a potential drug target. We developed a treatment plan based on comprehensive immunohistochemistry, a gene expression profile, and NGS analysis. The treatment plan was formulated using paclitaxel albumin and capecitabine in combination with radiotherapy. The efficacy evaluation was the partial response (PR) after four cycles of chemotherapy and two cycles combined with radiotherapy.Conclusion: This case highlighted the importance of identifying accurate primary tumor location for patients to benefit from treatment, which will provide a reference for the treatment decisions of CUP tumors in the future.</p

Additional file 3 of Characterization of SARS-CoV-2 worldwide transmission based on evolutionary dynamics and specific viral mutations in the spike protein

Additional file 3: Figure S2. Network graphic of SARS-CoV-2 isolates worldwide during 9 and 31 March 2020. Isolates were aligned by the Force Atlas model in Gephi. In the network, each node represented an isolate of SARS-CoV-2. Each color represented a country. Lines inherit colors from their origin clades. Distances between clades represented evolutionary distance

Additional file 6 of Characterization of SARS-CoV-2 worldwide transmission based on evolutionary dynamics and specific viral mutations in the spike protein

Additional file 6: Figure S5. Network graphic of SARS-CoV-2 isolates worldwide during 1 July and 31 August 2020. Isolates were aligned by the Force Atlas model in Gephi. In the network, each node represented an isolate of SARS-CoV-2. Each color represented a country. Lines inherit colors from their origin clades. Distances between clades represented evolutionary distance

Additional file 2 of Characterization of SARS-CoV-2 worldwide transmission based on evolutionary dynamics and specific viral mutations in the spike protein

Additional file 2: Figure S1. DNA sequencing to verify V367F and N354D mutations in the pseudovirus genome

Additional file 4 of Characterization of SARS-CoV-2 worldwide transmission based on evolutionary dynamics and specific viral mutations in the spike protein

Additional file 4: Figure S3. Network graphic of SARS-CoV-2 isolates worldwide during 1 and 30 April 2020. Isolates were aligned by the Force Atlas model in Gephi. In the network, each node represented an isolate of SARS-CoV-2. Each color represented a country. Lines inherit colors from their origin clades. Distances between clades represented evolutionary distance