Field and Pretreatment-Free Detection of Heavy-Metal Ions in Organic Polluted Water through an Alkyne-Coded SERS Test Kit

Field and pretreatment-free detection of heavy-metal ions in organic polluted water is important but still challenging in current water pollution emergency response systems. Here we report a polyadenine–DNA-mediated approach for a rationally designed alkyne-coded surface-enhanced Raman scattering (SERS) test kit, enabling rapid and simultaneous detection of Hg2+ and Ag+ by a portable spectrometer, impervious to organic interferences. Because of the formation of thymine (T)–Hg2+–T and cytosine (C)–Ag+–C, highly recognizable SERS signals are rapidly detected when two different alkyne-labeled gold nanoparticles (AuNPs) are induced to undergo controllable bridging upon the addition of low-volume targets. For multiplex detection through a portable spectrometer, the limits of detection reach 0.77 and 0.86 nM for Hg2+ and Ag+, respectively. Of particular significance, the proposed CC-containing Raman reporters provide an extremely effective solution for multiplex sensing in a spectral silent region, when the hyperspectral and fairly intense optical noises originating from lower wavenumber region (–1) are inevitable under complex ambient conditions

Biocatalytic Valorization of Biobased 5‑Hydroxymethylfurfural to 5‑Hydroxymethyl-2-furfurylamine in a Three-Constituent Deep Eutectic Solvent–Water System

5-Hydroxymethyl-2-furfurylamine (HMFA), as an important 5-hydroxymethylfurfural (5-HMF) derivative, has many potential applications in the preparation of diuretics, antihypertensive drugs, preservatives, and curing agents. In this work, highly efficient valorization of biomass-derived d-fructose into HMFA was attempted via a cascade reaction in a three-constituent deep eutectic solvent (3c-DES)–H2O. Using d-fructose (63.0 g/L) as a raw material, the yield of 5-HMF reached 68.2% in 3c-DES malic acid/glycerol/betaine–H2O (9:91, wt/wt) (pH 2.3) at 170 °C in 30 min. Whole cells of Escherichia coli CV harboring transaminase were utilized to valorize biomass-derived 5-HMF to HMFA at pH 8.0 and 30 °C using amine donor l-alanine. The valorization of 600 mM commercial 5-HMF gave a 93.2% yield of HMFA with 99.2% selectivity. In addition, CV cells transformed d-fructose-valorized 5-HMF into HMFA in 94.1% yield in 3c-DES malic acid/glycerol/betaine–H2O. This approach provided a sustainable and eco-efficient idea for chemoenzymatically valorizing biomass-derived d-fructose to HMFA

Supplemental Figure legends from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Legends of supplemental figure 1, 2, 3</p

Supplementary results from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Additional Analysis not included in the main text</p

Supplemental Figure 3 from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Interactive 3D plot showing the correlation of module 2 expression with CD14, IL-10, MDC and MCP-1.Each dot represents one DC sample. X, y and z coordinates represent concentration levels of IL-10, MDC, and MCP-1, respectively, size of dots is proportional with % of CD14+ cells, color represents module 2 expression level (red-yellow-white gradient, with red being the lowest expression level and white the highest)</p

Supplemental Figure 1 from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Patient Baseline Parameters and their correlation with Clinical Response measurements</p

Image1_Evaluation and management of symptomatic duodenal diverticula: a single-center retrospective analysis of 647 patients.tif

AimsTo explore the clinical characteristics of patients with symptomatic duodenal diverticula and to generalize how to make appropriate treatment choices for this group of patients.Materials and methodsFrom January 2010 to September 2020, a total of 647 patients with duodenal diverticula (DD) were included in this study. 345 of them with relevant symptoms were divided into the symptomatic group and the other 302 patients were in the asymptomatic group.ResultsAmong all patients, most DD were located in the periampullary area, ConclusionsPatients with DD ≥1 cm or located in the periampullary were more likely to be symptomatic. The specific size of the DD and the combination of specific biliary comorbidities may have an impact on the choice of treatment modality.</p

Supplemental Figure 2 from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Response Predictive Ability of secreted IL-12/IL-10 ratio</p

Supplemental file 1 from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Excel Spreadsheet listing all the genes belonging to the 8 modules</p

Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis-1

S from 5 subjects, AMD3100 (A)-mobilized CD133+ cells from 4 subjects and AMD3100 plus G-CSF (A+G)-mobilized CD133+ cells from 4 subjects. cDNA expression was analyzed using an expression microarray with 17,500 cDNA. Unsupervised hierarchical clustering of Eisen was used to analyze the 11,023 genes that remained after filtering (cDNA expressed in ≥ 80% of samples).<p><b>Copyright information:</b></p><p>Taken from "Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis"</p><p>http://www.translational-medicine.com/content/6/1/39</p><p>Journal of Translational Medicine 2008;6():39-39.</p><p>Published online 22 Jul 2008</p><p>PMCID:PMC2503968.</p><p></p