Data_Sheet_1_Real-time depth completion based on LiDAR-stereo for autonomous driving.PDF

The integration of multiple sensors is a crucial and emerging trend in the development of autonomous driving technology. The depth image obtained by stereo matching of the binocular camera is easily influenced by environment and distance. The point cloud of LiDAR has strong penetrability. However, it is much sparser than binocular images. LiDAR-stereo fusion can neutralize the advantages of the two sensors and maximize the acquisition of reliable three-dimensional information to improve the safety of automatic driving. Cross-sensor fusion is a key issue in the development of autonomous driving technology. This study proposed a real-time LiDAR-stereo depth completion network without 3D convolution to fuse point clouds and binocular images using injection guidance. At the same time, a kernel-connected spatial propagation network was utilized to refine the depth. The output of dense 3D information is more accurate for autonomous driving. Experimental results on the KITTI dataset showed that our method used real-time techniques effectively. Further, we demonstrated our solution's ability to address sensor defects and challenging environmental conditions using the p-KITTI dataset.</p

Development of Cyclic Tetrasiloxane Polymer as a High-Performance Dielectric and Hydrophobic Layer for Electrowetting Displays

Cyclic tetrasiloxane polymer (CTP) has recently garnered interest as a hydrophobic material with unique properties. This study aims to enhance the dielectric constant of CTP films by introducing excess Si–H groups and to explore the impact of synthesis and processing conditions on the resulting properties. The film demonstrates high hydrophobicity, with contact angles of 107° in air and 165° in n-decane, along with a notable dielectric constant of 5.1°. Furthermore, the CTP film displays reversible electrowetting behavior with low contact angle hysteresis (2°) and possesses good transparency (∼99%) and thermal stability. As such, the CTP film has significant potential as a material for the electric wetting of hydrophobic dielectric layers and may serve as a promising alternative in electrowetting applications

Novel α‑l‑Arabinofuranosidase from Cellulomonas fimi ATCC 484 and Its Substrate-Specificity Analysis with the Aid of Computer

In the process of gene mining for novel α-l-arabinofuranosidases (AFs), the gene <i>Celf_3321</i> from Cellulomonas fimi ATCC 484 encodes an AF, termed as AbfCelf, with potent activity, 19.4 U/mg under the optimum condition, pH 6.0 and 40 °C. AbfCelf can hydrolyze α-1,5-linked oligosaccharides, sugar beet arabinan, linear 1,5-α-arabinan, and wheat flour arabinoxylan, which is partly different from some previously well-characterized GH 51 AFs. The traditional substrate-specificity analysis for AFs is labor-consuming and money costing, because the substrates include over 30 kinds of various 4-nitrophenol (PNP)-glycosides, oligosaccharides, and polysaccharides. Hence, a preliminary structure and mechanism based method was applied for substrate-specificity analysis. The binding energy (Δ<i>G</i>, kcal/mol) obtained by docking suggested the reaction possibility and coincided with the experimental results. AbfA crystal 1QW9 was used to test the rationality of docking method in simulating the interaction between enzyme and substrate, as well the credibility of the substrate-specificity analysis method in silico

Additional file 1 of Vitamin D modulation of brain-gut-virome disorder caused by polystyrene nanoplastics exposure in zebrafish (Danio rerio)

Additional file 1: Text S1. TEM analysis. Text S2. Determination of biochemical parameters. Text S3. Gut Virome Analysis. Figure S1. Accumulation of PS-NPs in the brain tissues of zebrafish and changes of related growth parameters (0.2 μm). (A) TEM observation of PS-NPs in brain tissue, (a), (b), (c), (d), (e), and (f) represent the group of 0-, 0+, 15-, 15+, 150-, and 150+, respectively, the blue arrow points to the NPs; (B) The number of NPs in brain tissue (n=3 replicates); (C) BSI (%). Data are expressed as means±SD. *p<0.05 indicate significant differences between the exposure groups and the control group. Figure S2. (A) Average velocity (mm/s); (B) Average acceleration (mm/s2); (C) and (D) represents the content of cortisol and OT in zebrafish brain samples. Data are expressed as means±SD. *p<0.05 indicate significant differences between the exposure groups and the control group. Figure S3. (A) Relative abundance of bacteria at the genus level (top 10) (n=3 replicates); (B) The relative abundance of Exiguobacterium. Data are expressed as means±SD. *p<0.05 indicate significant differences between exposure groups and the control group; #p<0.05 indicate significant differences between vitamin D-high and vitamin D-low groups at the same PS-NPs concentration. Table S1. Differentially expressed virus in 15+ vs 15- comparison. Table S2. Differentially expressed virus in 150+ vs 150- comparison. Table S3. Primer information used in qRT-PCR. All sequences are shown 5’-3’

DataSheet1_Comprehensive Analysis of Circular RNA Expression in ceRNA Networks and Identification of the Effects of hsa_circ_0006867 in Keloid Dermal Fibroblasts.DOCX

Circular RNAs (circRNAs) play a crucial role in the pathogenesis of various fibrotic diseases, but the potential biological function and expression profile of circRNAs in keloids remain unknown. Herein, microarray technology was applied to detect circRNA expression in four patient-derived keloid dermal fibroblasts (KDFs) and normal dermal fibroblasts (NDFs). A total of 327 differentially expressed (DE) circRNAs (fold change > 1.5, p < 0.05) were identified with 195 upregulated and 132 downregulated circRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that the upregulated circRNAs were mainly enriched in the cytoskeleton and tight junctions, while the downregulated circRNAs were related to morphogenesis of the epithelium and axonal guidance. To explore the function of DE circRNAs, a circRNA-miRNA-mRNA network, including five circRNAs, nine miRNAs, and 235 correlated mRNAs, was constructed using bioinformatics analyses. The expression of five DE circRNAs was validated by qRT–PCR in 18 pairs of KDFs and NDFs, and hsa_circ_0006867 showed promising regulatory function in keloids in vitro. Silencing hsa_circ_00006867 suppressed the proliferation, migration, and invasion of keloid fibroblasts. RNA-binding protein immunoprecipitation (RIP) assays indicated that hsa_circ_00006867 may serve as a platform for miRNA binding to Argonaute (AGO) 2. In addition, hsa-miR-29a-5p may be a potential target miRNA of hsa_circ_00006867. Taken together, our research provided multiple novel clues to understand the pathophysiologic mechanism of keloids and identified hsa_circ_0006867 as a biomarker of keloids.</p

Thermal-Oxidation-Resistant Poly(Carborane-Silane) for Protective Coatings Under Harsh Environments

Thermal-oxidation-resistant polymers are critical for devices/components used under harsh environments where high temperature and an oxidative atmosphere lead to degradation and failure of polymeric parts. Herein, vinyl-containing poly(carborane-silane) (VCP) with good thermal-oxidative stability has been prepared through vinyl-modified m-carborane and silane. The cured VCP (c-VCP) could resist high temperature in both inert and oxidative atmospheres. In comparison to traditional carborane-siloxane copolymers, c-VCP aimed at lowering the oxygen content and simultaneously increasing the boron content, allowing maximum ability to capture oxygen atoms. In an oxidative atmosphere, the formation of the boron oxide layer brought nearly 45% weight gain rather than a weight decrease, which protected the matrix from thermal-oxidative degradation. The mechanism of thermal-oxidative degradation was also investigated based on Flynn–Wall–Ozawa method. The boron oxide layer increased the degradation activation energy (Ea) and hindered direct contact of inner materials with oxygen atoms. When the carbon fibers were coated with c-VCP, the char yield could be increased from 0 to 57 wt % at 1000 °C in air, which demonstrated that c-VCP coating could effectively protect carbon fiber from oxidation at high temperature in air, also indicating potential applications as anti-thermal-oxidative materials for harsh environments

Characterization of a Novel α‑l‑Arabinofuranosidase from Ruminococcus albus 7 and Rational Design for Its Thermostability

An α-l-arabinofuranosidase (Abf) encoding gene was obtained via genomic mining from a Ruminococcus albus strain. The specific activity of this GH 51 Abf was 73.3 U/mg at pH 6.0 and 50 °C. The modification of Abf, aimed at improving thermostability, was performed through different strategies. Structure-based rational design using the PoPMuSiC and the Enzyme Thermal Stability System (ETSS) predicted thermal stability of Abf and enhanced the half-life of thermal inactivation (t1/2) at 50 °C for K208W more than 11.1 times versus the wild-type (WT). Sequence-based rational design was also conducted by substituting histidine with lysine at various sites. Among eight mutants, the t1/2 at 50 °C of H337K was prolonged by 5.0-fold, and the specific activity of this mutant was increased to 121.8 U/mg. In addition, the mutant H337K was utilized with some enzymes to extract pectin from apple pomace. The enzymatically produced pectin got less moisture and ash, milder pH, and higher viscosity than its acid-extracted counterpart, indicating that Abf has an application prospect in pectin production

Table1_Comprehensive Analysis of Circular RNA Expression in ceRNA Networks and Identification of the Effects of hsa_circ_0006867 in Keloid Dermal Fibroblasts.pdf

Circular RNAs (circRNAs) play a crucial role in the pathogenesis of various fibrotic diseases, but the potential biological function and expression profile of circRNAs in keloids remain unknown. Herein, microarray technology was applied to detect circRNA expression in four patient-derived keloid dermal fibroblasts (KDFs) and normal dermal fibroblasts (NDFs). A total of 327 differentially expressed (DE) circRNAs (fold change > 1.5, p < 0.05) were identified with 195 upregulated and 132 downregulated circRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that the upregulated circRNAs were mainly enriched in the cytoskeleton and tight junctions, while the downregulated circRNAs were related to morphogenesis of the epithelium and axonal guidance. To explore the function of DE circRNAs, a circRNA-miRNA-mRNA network, including five circRNAs, nine miRNAs, and 235 correlated mRNAs, was constructed using bioinformatics analyses. The expression of five DE circRNAs was validated by qRT–PCR in 18 pairs of KDFs and NDFs, and hsa_circ_0006867 showed promising regulatory function in keloids in vitro. Silencing hsa_circ_00006867 suppressed the proliferation, migration, and invasion of keloid fibroblasts. RNA-binding protein immunoprecipitation (RIP) assays indicated that hsa_circ_00006867 may serve as a platform for miRNA binding to Argonaute (AGO) 2. In addition, hsa-miR-29a-5p may be a potential target miRNA of hsa_circ_00006867. Taken together, our research provided multiple novel clues to understand the pathophysiologic mechanism of keloids and identified hsa_circ_0006867 as a biomarker of keloids.</p

Systemic Stereoselectivity Study of Fenobucarb: Environmental Behaviors in Greenhouse Vegetables, Fruits, Earthworms, and Soils and Its Cytotoxicity

Fenobucarb (2-sec-butylphenyl methylcarbamate, BPMC) is a potent carbamate pesticide with high insecticidal activity. In this study, the enantioselective accumulation of BPMC in earthworms (Eisenia foetida) and dissipation in cabbage, Chinese cabbage, strawberry, and soils were investigated. The samples were prepared using the QuEChERS method and analyzed using fast and sensitive chiral high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) analysis. The stereoselective accumulation of BPMC enantiomers revealed that S-(+)-BPMC was preferentially accumulated in earthworms rather than its antipode. However, the dissipation studies showed that S-(+)-BPMC degraded faster than the R-(−)-isomer in cabbage, Chinese cabbage, strawberry, and soils. Furthermore, the cytotoxic effect of BPMC enantiomers toward PC12 and N9 neuronal, A549 lung cancer, and MRC5 lung fibroblast cell lines was evaluated using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Compared with R-(−)- and rac-isomers, S-(+)-BPMC exhibited lower cytotoxicity in neuronal cells and a weaker proliferating effect on lung cancer and lung fibroblast cells. Altogether, the findings suggest the use of the pure S-(+)-enantiomer in agricultural management rather than the use of the racemate or the R-(−)-isomer, which might reduce the environmental risk

DataSheet_1_SETD3 Methyltransferase Regulates PLK1 Expression to Promote In Situ Hepatic Carcinogenesis.docx

BackgroundThe development of a new strategy to overcome chemoresistance to hepatocellular carcinoma (HCC) treatment is a long-standing issue. We have previously found that upregulated SETD3 levels are closely correlated with HCC. This study aims to explore the mechanism underlying how upregulation of SETD3 promotes liver carcinogenesis.MethodsRNA-Sequencing analysis was used to explore the correlation of SETD3 with regulatory targets. In vitro assays including cell proliferation and migration were performed to study the oncogenic roles of SETD3 and PLK1. Western blotting, immunohistochemical staining, and blood biochemical assays were performed to examine protein expression or pathological index in tumor tissues and mice liver tissues. Luciferase reporter system and chromatin immunoprecipitation assays were used to explore the mechanism.ResultsWe revealed that SETD3 regulates gene expression in subgroups, including cell division, cell proliferation, and cell cycle, in hepatocellular tumor cells. We found that SETD3 upregulation is associated with elevated PLK1 level in both hepatic tumor cells and clinical liver tissues. We further showed that overexpression of SETD3 promoted tumor cell proliferation and migration, whereas inhibition of PLK1 activity attenuated these phenotypes caused by SETD3. By taking advantage of the Sleep Beauty transposase system, we confirmed that upregulated mouse Setd3 promoted hepatic carcinogenesis in situ, but knockdown of mouse Plk1 mitigated Setd3-promoted tumorigenesis in mice. Mechanistically, we showed that SETD3 could be recruited to the promoter of PLK1 gene to facilitate PLK1 transcription.ConclusionsOur data demonstrate that elevated SETD3 may promote HCC by enhancing PLK1 expression, which suggests that SETD3 may act as a potential drug target combined with PLK1 inhibition to treat HCC.</p