High-Performance Recovery of Vanadium(V) in Leaching/Aqueous Solution by a Reusable Reagent-Primary Amine N1519

Efficient extraction and stripping for recovering vanadium­(V) from the leaching/aqueous solution of chromium-bearing vanadium slag (V–Cr slag) are essential to the reuse of heavy metals. The performance characteristics of a new reagent, primary amine N1519, were first reported for extracting vanadium. With a phase ratio of organic to aqueous up to 1:1, 99.7% of vanadium­(V) can be effectively extracted from the leaching/aqueous solution, and powder of NH₄VO₃ was obtained through the stripping with ammonia. The new reagent can be recyclable in use for sustainable reuse after stripping. Different extraction conditions, e.g., the initial pH of the leaching/aqueous solution and the molar quantity of N1519 were investigated. The powder of vanadium-organic compounds (VOC) with N1519 formed in the process of extraction was obtained and purified through three-steps of solvent-out crystallizations. The hydrogen bond association mechanism of extraction was illustrated with the structure of VOC and the enthalpy change in extraction process. The fast extraction process and slow stripping procedure for recovering vanadium­(V) are suitable for use in annular centrifugal contactors with very short contact/resident times and mixed-settler extractors with very good mass transfer, respectively. The results offer significant advantages over conventional processes

DataSheet_1_Identification of a Novel SBP1-Containing SCFSFB Complex in Wild Dwarf Almond (Prunus tenella).docx

S-RNase-based gametophytic self-incompatibility (SI), in which specificities of pistil and pollen are determined by S-RNase and the S locus F-box protein, respectively, has been discovered in the Solanaceae, Plantaginaceae, and Rosaceae families, but some underlying molecular mechanisms remain elusive and controversial. Previous studies discovered SI in wild dwarf almond (Prunus tenella), and pistil S (S-RNase) and pollen S (SFB) determinant genes have been investigated. However, the SCF (SKP1–Cullin1–F-box-Rbx1) complex, which serves as an E3 ubiquitin ligase on non-self S-RNase, has not been investigated. In the current study, PetSSK1 (SLF-interacting-SKP1-like1), SBP1 (S-RNase binding protein 1), CUL1, and SFB genes (S-haplotype-specific F-box) were identified in an accession (ZB1) of P. tenella. Yeast two-hybrid assays revealed interactions between PetSBP1 and PetCUL1 and between PetSBP1 and PetSFBs (SFB16 and SFB17), and subsequent pull-down assays confirmed these interactions, suggesting a novel SBP1-containing SCFSFB complex in wild dwarf almond. Moreover, despite a putative interaction between PetSSK1 and PetCUL1, we revealed that PetSSK1 does not interact with PetSFB16 or PetSFB17, and thus the canonical SSK1-containing SCFSFB complex could not be identified. This suggests a novel molecular mechanism of gametophytic SI in Prunus species.</p

Additional file 1 of Chemoproteomics-based profiling reveals potential antimalarial mechanism of Celastrol by disrupting spermidine and protein synthesis

Additional file 1: Fig. S1. The antimalarial activity of Cel and Cel-P against P. falciparum Dd2 strain. Fig. S2. Heatmap representation of the proteome after Celastrol treatment. Fig. S3. The absorbance spectra of increasing concentration Celastrol. Fig. S4. A Heatmap representation of the decreased expression of parasite proteins after Cel treatment. B GO enrichment analysis of the decreased expression proteins. Fig. S5. The antimalarial activity of Cel against artemisinin-sensitive (P. falciparum 3D7) and artemisinin-resistant strains (P. falciparum 6320). Fig. S6. Raw data of all gel images and Western blots