Silk Fibroin-Based Scaffolds with Controlled Delivery Order of VEGF and BDNF for Cavernous Nerve Regeneration

To investigate the synergistic effect of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) on cavernous nerve regeneration, two different aligned scaffolds consisting of coaxial electrospun silk fibers were prepared by switching the position of the two factors in either core or shell domain. The order and release rate of the dual factors delivery were relatively different because of the distinct location of two factors in coaxial fibers. An in vitro assay showed that the inner-VEGF/outer-BDNF scaffolds could more obviously accelerate Schwann cells growth, proliferation and spreading owing to the rapid release of BDNF. However, in vivo scaffold implantation demonstrated that the inner-BDNF/outer-VEGF scaffolds significantly facilitated more angiogenesis, and promoted more nerve regeneration based on great benefit of angiogenesis. Results showed that the reasonable dual-delivery order of VEGF and BDNF from scaffolds could enhance synergistic effect of the factors and promote cavernous nerve regeneration

Neutral Mononuclear Copper(I) Complexes: Synthesis, Crystal Structures, and Photophysical Properties

Neutral green-emitting four-coordinate Cu­(I) complexes with general formula POPCu­(NN), where POP = bis­[2-(diphenylphosphino)­phenyl]­ether and NN = substituted 2-pyridine-1,2,4-triazole ligands, were synthesized. The crystal structures of (POPCuMeCN)⁺(PF₆)⁻ (<b>1</b>), POPCuPhPtp (<b>2a</b>, PhPtp = 2-(5-phenyl-2<i>H</i>-[1,2,4]­triazol-3-yl)-pyridine), and POPCu­(3,5-2FPhPtp) (<b>2d</b>, 3,5-2FPhPtp = 2-(5-(3,5-difluorophenyl)-2<i>H</i>-1,2,4-triazol-3-yl)­pyridine) were determined by single-crystal X-ray diffraction analysis. The electronic and photophysical properties of the complexes were examined by UV–vis, steady-state, and time-resolved spectroscopy. At room temperature, weak emission was observed in solution, while in the solid state, all complexes exhibit intense green emission with quantum yield up to 0.54. The electronic and photophysical properties were further supported by calculation performed at the (time-dependent) density functional theory level. One of the complexes was also tested as dopant in electroluminescent devices

Additional file 1 of Model containing sarcopenia and visceral adiposity can better predict the prognosis of hepatocellular carcinoma: a multicenter study

Additional file 1