A typical desialylated N-glycan profile of serum protein.

<p>At least 9 peaks can be identified. Peaks 1, 2, 5 and 9 increased (red arrows), and peaks 3, 6 and 7 decreased (green arrows) in gastric carcinoma compared with normal controls. The structures of the N-glycan peaks are shown below the chart. The open circles indicate b-linked galactose; the triangles, a/b-1,3/6-linked fucose; and the solid circles, a/b-linked mannose.</p

Receiver operating characteristic (ROC) curve analyses for the prediction of gastric carcinoma (GC).

<div><p>(A) The ROC analysis for distinguishing between GC and control subjects using an N-glycan marker-based GC diagnostic model (GCglycoA), CEA, CA19-9, CA125 or CA72-4. The areas under the ROC curve (AUCs) indicate the diagnostic power: CEA (0.74), CA19-9 (0.76), CA125 (0.72), CA72-4 (0.67) and GCglycoA (0.88). The diagnostic model was constructed by using forward stepwise logistic regression analysis:</p> <p>GCglycoA = -1.072+0.957peak4-0.331peak6+0.646peak9. (B) The ROC analysis for distinguishing between GC and atrophic gastritis using the GCglycoB diagnostic model, CEA, CA19-9, CA125 or CA72-4. The AUCs indicate the diagnostic power: GCglycoB (0.82), CEA(0.65), CA19-9 (0.63), CA125 (0.69) and CA72-4 (0.64). The diagnostic model was constructed by using forward stepwise logistic regression analysis: GCglycoB=5.273-1.371peak2+0.781peak4-0.453peak6+0.221peak9.</p></div

The abundance of total core fucosylated residues, α-1,6-fucosyltransferase (Fut8) and guanosine diphosphate (GDP)-fucose transporter (GDP-fuc-Tr) using lectin blotting and reverse transcription-polymerase chain reaction (RT-PCR).

<p>(A) Lectin blots of serum proteins probed with lens culinaris agglutinin A (LCA). The horizontal axis represents the experimental groups: control (n=20), atrophic gastritis (n=20), and gastric carcinoma (GC) (n=20); each pool consists of 3 homogenous samples. The vertical axis indicates the ratio of fucosylated protein to total protein. The difference between the groups was statistically significant (P <0.001). (B) Lectin blotting of tissue proteins probed with LCA. The horizontal axis represents the experimental groups: tumor tissue (n=20) and adjacent tissue (n=20). The vertical axis indicates the ratio of fucosylated protein to total protein. The difference between the groups was not statistically significant (P >0.05). (C) The relative messenger RNA (mRNA) expression of Fut8 in tissue as measured by RT-PCR. The horizontal axis represents the experimental groups: tumor tissue (n=20) and adjacent tissue (n=20). The vertical axis indicates the relative expression of Fut8. The difference between the groups was statistically significant (P <0.001). (D) The relative mRNA abundance of GDP-fuc-Tr in tissue as measured by RT-PCR. The horizontal axis represents the experimental groups: tumor tissue (n=20) and adjacent tissue (n =20). The vertical axis indicates the relative abundance of GDP-fuc-Tr. The difference between the groups was not statistically significant (P >0.05).</p

Demographic and baseline characteristics of the two patient groups.

<p>Demographic and baseline characteristics of the two patient groups.</p

Univariate analysis of the prognostic factors for gastric cancer patients using the Cox regression model.

<p>Univariate analysis of the prognostic factors for gastric cancer patients using the Cox regression model.</p

The differences of D-dimer levels.

<p>(A) Plasma levels of D-dimer in patients with gastric cancer (1.25±1.08 µg/mL) were significantly higher than the values determined for control subjects (0.37±0.20 µg/mL) (P<0.001). (B) The mean plasma D-dimer level of patients with peritoneal dissemination was 2.20±1.51 µg/mL, a value that was significantly higher than the measured amount for patients without peritoneal dissemination (1.01±0.79 µg/mL) (P<0.001). (C) The mean plasma D-dimer level in surviving patients was 0.79±0.720 µg/mL, a value that was significantly lower than the amount determined for the deceased patients (1.36±1.13 µg/mL) (P<0.001).</p

Correlation between the plasma D-dimer levels and the clinicopathologic factors of gastric cancer patients.

<p>Correlation between the plasma D-dimer levels and the clinicopathologic factors of gastric cancer patients.</p

Pathophysiology of D-dimer.

<p>Stage I was the process of blood coagulation, Stage II was the process of fibrinolysis, as the degradation product of fibrin, D-dimer can promote the growth and metastasis of tumors.</p

Plasma D-dimer and OS.

<p>(A) Kaplan-Meier curve for OS for gastric cancer patients stratified by peritoneal dissemination. Log-rank test, P<0.001 vs patients without peritoneal dissemination. (B) Kaplan-Meier curve for OS for gastric cancer patients stratified by plasma D-dimer levels (<1.465 µg/ml). Log-rank test, P<0.001 vs patients with plasma D-dimer levels ≥1.465 µg/ml.</p

The diagnostic power of D-dimer in differentiating peritoneal dissemination from the non-peritoneal dissemination in the Prospective Training Group.

<p>Abbreviations: PD, peritoneal dissemination; +, positive; −, negative; PPV, positive predictive value; NPV, negative predictive value;.</p