Effect of Primary Particle Size and Salt Concentration on the Structure of Colloidal Gels

Structures of colloidal gels prepared in the reaction-limited aggregation regime from fluorinated colloidal particles with two different sizes and different salt concentrations have been characterized by combining small-angle and wide-angle light scattering and small-angle neutron scattering techniques. In all cases, the scattering structure factors indicate the coexistence of two types of fractal structures at different scale lengths: a mass fractal scaling at the scale length of the clusters that constitute the gel and a surface fractal scaling for larger scale lengths. It has been found that, at the same particle volume fraction, the mass fractal dimension of the clusters in the gel increases as the primary particle size decreases. On the other hand, we show that both the mass and the surface fractal gel structures are independent of the salt concentration

DataSheet1_In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds.PDF

Background: In recent years, the impact of bacterial biofilms on traumatic wounds and the means to combat them have become a major research topic in the field of medicine. The eradication of biofilms formed by bacterial infections in wounds has always been a huge challenge. Herein, we developed a hydrogel with the active ingredient berberine hydrochloride liposomes to disrupt the biofilm and thereby accelerate the healing of infected wounds in mice.Methods: We determined the ability of berberine hydrochloride liposomes to eradicate the biofilm by means of studies such as crystalline violet staining, measuring the inhibition circle, and dilution coating plate method. Encouraged by the in vitro efficacy, we chose to coat the berberine hydrochloride liposomes on the Poloxamer range of in-situ thermosensitive hydrogels to allow fuller contact with the wound surface and sustained efficacy. Eventually, relevant pathological and immunological analyses were carried out on wound tissue from mice treated for 14 days.Results: The final results show that the number of wound tissue biofilms decreases abruptly after treatment and that the various inflammatory factors in them are significantly reduced within a short period. In the meantime, the number of collagen fibers in the treated wound tissue, as well as the proteins involved in healing in the wound tissue, showed significant differences compared to the model group.Conclusion: From the results, we found that berberine liposome gel can accelerate wound healing in Staphylococcus aureus infections by inhibiting the inflammatory response and promoting re-epithelialization as well as vascular regeneration. Our work exemplifies the efficacy of liposomal isolation of toxins. This innovative antimicrobial strategy opens up new perspectives for tackling drug resistance and fighting wound infections.</p

Grayanane Diterpenoids from the Flowers of <i>Rhododendron molle</i> with Cytotoxic Activity against a <i>Spodoptera frugiperda </i>Cell Line

Two new grayanane diterpenoids, rhodomolins A (1) and B (2), together with two known diterpenoids, rhodomolleins I and rhodojaponin III, were isolated from the flowers of Rhododendron molle. The structures of 1 and 2 were elucidated on the basis of interpretation of spectroscopic data. All compounds were evaluated for cytotoxic activity against the Spodoptera frugiperda cell line Sf-9 and gave IC50 values in the range 12−80 μg/mL

Development of a mass spectrometry-based metabolomics workflow for traceability of wild and cultivated <i>Cordyceps sinensis</i>

Cordyceps sinensis, as an expensive traditional Chinese medicine and edible fungus mycelium, lacks an effective quality evaluation method, especially and cultivated Cordyceps sinensis. In this study, a feasible workflow method was developed for traceability evaluation of wild and cultivated Cordyceps sinensis, based on mass spectrometry-based metabolomics. Mass spectrometry data were firstly acquired from Cordyceps sinensis, samples by liquid chromatography-quadrupole and time of flight mass spectrometry. Characteristic mass spectrometry peaks were extracted by applying the MZmine. Then significant markers were obtained from Cordyceps sinensis samples by orthogonal partial least square discriminant analysis. Then, identification of significant markers were identified by MS-FINDER data analytics. The results showed that Changdu, the other four wild origins (Naqu, Xinghai, Yushu and Guoluo) and cultivated samples could be significantly distinguished. This identified significant markers of Cordyceps sinensis, including 174 special significant markers for the wild samples, 204 special significant markers for the cultivated samples and 87 share significant markers. Number of 87 shared significant markers were identified in the wild and cultivated Cordyceps sinensis, especially 28 confident significant compounds, such as adenosine, riboflavin, tyrosine, arginine and glutamine. These shared significant markers might support the quality control of multi-targets of Cordyceps sinensis, compared with a single target in the Chinese Pharmacopoeia. The special significant markers indicated that cultivated Cordyceps sinensis was different from the wild based on mass spectrometry-based metabolomics. In the comparison of chromatographic fingerprint technology, it was found that the established feasible workflow method was easy to acquire significant markers and traceability of Cordyceps sinensis. This feasible workflow method has great potential to be successful for comprehensive and traceability evaluation of the wild and cultivated Cordyceps sinensis.</p

Additional file 1 of Identification of PANoptosis-related signature reveals immune infiltration characteristics and immunotherapy responses for renal cell carcinoma

Supplementary Material

Characteristics of the patients on both groups.

<p>Characteristics of the patients on both groups.</p

Calculusafter ESWL.

<p>Calculusafter ESWL.</p

Stone clearance rate according to ESWL sessions.

<p>ESWL1∶1^st session ESWL and so far.</p

Calculus-freeafter ureteroscopy.

<p>Calculus-freeafter ureteroscopy.</p

Detailed procedural indexes of patients.

<p>Note: “overall procedural time” for ESWL include the repeated procedures in some cases; “overall procedural time” for ureteroscopy included anesthesia time, operation time and hospitalization time.</p