Editor\u27s Notes, Chiba Medical Journal 90-1

Multiple sequence alignment of deduced amino acid sequences of 25 wheat annexin genes with rice annexin OsAnn2 (Os05g31760) obtained by ClustalW. (PDF 212 kb

Image_1_Primary pure large cell neuroendocrine carcinoma of the urinary bladder: a case report and literature review.tif

BackgroundThe large cell neuroendocrine carcinoma (LCNEC) of the urinary bladder is a rare malignancy. With its high aggressiveness and poor prognosis, the disease is often accompanied by metastasis or recurrence. The lack of specific clinical manifestations and imaging features causes considerable challenges for clinical diagnosis and treatment.Case presentationWe report a case of LCNEC of the urinary bladder. The patient was a 79-year-old male admitted to our hospital with recurrent episodes of asymptomatic gross hematuria. Based on the computed tomography (CT) scan findings, our patient presented with a bladder mass displaying invasion into the serosal layer, suggestive of muscle involvement and indicative of malignancy. The patient received a radical cystectomy, and the postoperative pathology confirmed primary, pure LCNEC of the urinary bladder. We gave him 16 cycles of toripalimab immunotherapy. As of follow-up, the patient was alive, and periodic CT reexamination showed no evidence of recurrence.ConclusionsWe reviewed domestic and foreign literature and found no explicit treatment protocols exist for the disease. Surgical resection combined with chemotherapy were the most common treatments. Herein, we reported the first case of primary, pure LCNEC of the urinary bladder treated by radical cystectomy combined with pure immunotherapy, achieving sustained remission, which provides a new idea for the immunotherapy and integrative treatment of the disease.</p

Rapid Stress Relaxation, Multistimuli-Responsive Elastomer Based on Dual-Dynamic Covalent Bonds and Aniline Trimer

Covalent adaptable networks (CANs) are an emerging kind of smart materials in which cross-links are reversible upon some stimuli and then provide malleability and a stimuli-responsive ability to the materials. There is a trend to endow CANs with multistimuli-responsive capabilities and rapid stress relaxation to pursue more advanced applications. To integrate these two features into one material, here, dual-dynamic covalent bonds (imines and boronic esters) and aniline trimer (ACAT) were incorporated into the styrene butadiene elastomer as dynamic cross-links. The obtained CANs were demonstrated with rapid stress relaxation and a relatively low activation energy of 36 ± 1 kJ mol–1, resulting from the synergistic effect of dual-dynamic covalent bonds to rearrange the network at a faster rate than for either imines or boronic esters. Because of the dynamic nature of imines or boronic esters, the elastomer can be recycled upon heat. Moreover, the appearance and configuration of the elastomer could also be manipulated by pH and light because of the inclusion of ACAT. All in all, the coupled multistimuli-responsive behavior and rapid stress relaxation in one single elastomer would potentially be applicable for sensors and actuators with good recyclability

Table_2_Primary pure large cell neuroendocrine carcinoma of the urinary bladder: a case report and literature review.docx

BackgroundThe large cell neuroendocrine carcinoma (LCNEC) of the urinary bladder is a rare malignancy. With its high aggressiveness and poor prognosis, the disease is often accompanied by metastasis or recurrence. The lack of specific clinical manifestations and imaging features causes considerable challenges for clinical diagnosis and treatment.Case presentationWe report a case of LCNEC of the urinary bladder. The patient was a 79-year-old male admitted to our hospital with recurrent episodes of asymptomatic gross hematuria. Based on the computed tomography (CT) scan findings, our patient presented with a bladder mass displaying invasion into the serosal layer, suggestive of muscle involvement and indicative of malignancy. The patient received a radical cystectomy, and the postoperative pathology confirmed primary, pure LCNEC of the urinary bladder. We gave him 16 cycles of toripalimab immunotherapy. As of follow-up, the patient was alive, and periodic CT reexamination showed no evidence of recurrence.ConclusionsWe reviewed domestic and foreign literature and found no explicit treatment protocols exist for the disease. Surgical resection combined with chemotherapy were the most common treatments. Herein, we reported the first case of primary, pure LCNEC of the urinary bladder treated by radical cystectomy combined with pure immunotherapy, achieving sustained remission, which provides a new idea for the immunotherapy and integrative treatment of the disease.</p

Table_1_Primary pure large cell neuroendocrine carcinoma of the urinary bladder: a case report and literature review.docx

BackgroundThe large cell neuroendocrine carcinoma (LCNEC) of the urinary bladder is a rare malignancy. With its high aggressiveness and poor prognosis, the disease is often accompanied by metastasis or recurrence. The lack of specific clinical manifestations and imaging features causes considerable challenges for clinical diagnosis and treatment.Case presentationWe report a case of LCNEC of the urinary bladder. The patient was a 79-year-old male admitted to our hospital with recurrent episodes of asymptomatic gross hematuria. Based on the computed tomography (CT) scan findings, our patient presented with a bladder mass displaying invasion into the serosal layer, suggestive of muscle involvement and indicative of malignancy. The patient received a radical cystectomy, and the postoperative pathology confirmed primary, pure LCNEC of the urinary bladder. We gave him 16 cycles of toripalimab immunotherapy. As of follow-up, the patient was alive, and periodic CT reexamination showed no evidence of recurrence.ConclusionsWe reviewed domestic and foreign literature and found no explicit treatment protocols exist for the disease. Surgical resection combined with chemotherapy were the most common treatments. Herein, we reported the first case of primary, pure LCNEC of the urinary bladder treated by radical cystectomy combined with pure immunotherapy, achieving sustained remission, which provides a new idea for the immunotherapy and integrative treatment of the disease.</p

Table_3_Primary pure large cell neuroendocrine carcinoma of the urinary bladder: a case report and literature review.docx

BackgroundThe large cell neuroendocrine carcinoma (LCNEC) of the urinary bladder is a rare malignancy. With its high aggressiveness and poor prognosis, the disease is often accompanied by metastasis or recurrence. The lack of specific clinical manifestations and imaging features causes considerable challenges for clinical diagnosis and treatment.Case presentationWe report a case of LCNEC of the urinary bladder. The patient was a 79-year-old male admitted to our hospital with recurrent episodes of asymptomatic gross hematuria. Based on the computed tomography (CT) scan findings, our patient presented with a bladder mass displaying invasion into the serosal layer, suggestive of muscle involvement and indicative of malignancy. The patient received a radical cystectomy, and the postoperative pathology confirmed primary, pure LCNEC of the urinary bladder. We gave him 16 cycles of toripalimab immunotherapy. As of follow-up, the patient was alive, and periodic CT reexamination showed no evidence of recurrence.ConclusionsWe reviewed domestic and foreign literature and found no explicit treatment protocols exist for the disease. Surgical resection combined with chemotherapy were the most common treatments. Herein, we reported the first case of primary, pure LCNEC of the urinary bladder treated by radical cystectomy combined with pure immunotherapy, achieving sustained remission, which provides a new idea for the immunotherapy and integrative treatment of the disease.</p

DataSheet1_Synthesis of High-Molecular-Weight Branched Polyethylene Using a Hybrid “Sandwich” Pyridine-Imine Ni(II) Catalyst.docx

Most pyridine-imine Ni(II) and Pd(II) catalysts tend to yield low-molecular-weight polyethylene and ethylene-based copolymers in olefin insertion polymerization, as the unilateral axial steric structure of such complexes often cannot provide effective shielding of the metal center. In this study, we synthesized a series of hybrid “semi-sandwich” and “sandwich” type pyridine-imine Ni(II) complexes by incorporating diarylmethyl or dibenzosuberyl groups onto 8-aryl-naphthyl motif. The as-prepared Ni(II) complexes afforded highly branched polyethylene with high molecular weights (level of 105 g/mol), and moderate activities (level of 105 g/(molh)) in ethylene polymerization. Most interestingly, compared to “semi-sandwich” Ni(II) complexes bearing (2-diarylmethyl-8-aryl)naphthyl units, the “full-sandwich” counterpart containing (2-dibenzosuberyl-8-aryl)naphthyl motif was able to produce higher-molecular-weight polyethylene with higher branching density. In addition, the effect of remote non-conjugated electronic substituents in diarylmethyl groups of the Ni(II) system was also observed in ethylene polymerization.</p

Single-Phase Organic–Inorganic Hybrid Nanoparticles for Warm-White Lighting

White light-emitting diodes (W-LEDs) are an important solid-state lighting source, thanks to their superior properties. Here, a single-phase phosphor composed of organic–inorganic hybrid nanoparticles having a potential application in W-LEDs is successfully prepared. Green facile hydrothermal synthesis is used to obtain the phosphor integrated LaF3:Pr3+ with inexpensive sodium dodecyl benzene sulfonate (SDBS). It emits bright white light excited by ultraviolet (UV, λ = 365 nm) light. Moreover, its color coordinates and temperature are (0.365, 0.412) and 4606 K, respectively, according to the Commission Internationale de L’Eclairage (CIE). Meanwhile, phosphor shows good thermal stability and long photoluminescence decay time. Therefore, it would be a potential material for warm-white lighting

Morphological and immunohistochemical analysis of mutant mice.

<p>(<b><i>A</i></b><b>–</b><b><i>D</i></b>) Representative immunofluorescence staining with prestin (green) and myosin 6 (red) in whole-mount preparations of basal cochlear turns (corresponding to the 60 kHz region of the +/+ cochlea) in the indicated mouse genotypes at P24. 4′, 6-diamidino-2-phenylindole (DAPI) counterstain is shown in blue. Scale bar: 20 µm. <i>Neo/Neo</i> and <i>Neo/-</i> mice show no OHC loss or abnormal distribution of prestin. (<b><i>E</i></b>) Length of OHCs of each genotype at a given location corresponding to 16 kHz region of the +/+ cochlea. Values are the mean ± SEM; **: <i>P</i><0.01, *: <i>P</i><0.05. (<b><i>F</i></b>) Length of OHCs of each genotype at different locations of the cochlea. The shorter OHCs could reduce the mass of the organ of Corti and result in a higher frequency response for a given location, assuming all other material properties remain the same. The x-axis displays a normalized distance from apex (0%) to base (100%). That is, the locations responding to 4, 6, 12, 16, and 22 kHz in wild-type cochleae correspond to 4, 12, 30, 40, and 51% in a normalized distance from the cochlear apex, respectively. Calculated intercepts for each genotype differed significantly by one-way ANOVA, followed by Student's t test with a Bonferroni correction.</p

The relationship between prestin activity/feedback efficiency and amplification gain.

<p>The relationship between amplification gain and feedback efficiency proposed by Patuzzi et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045453#pone.0045453-Patuzzi1" target="_blank">[7]</a> is plotted as a black curve. Y-axes represent the input/output (y/x) ratio (left) and the amplification gain [dB SPL = 20log(y/x), right], respectively. The amplification gain (dB SPL) for each genotype is the ABR threshold difference between prestin <i>−/−</i> mice and wild-type, <i>Neo/Neo</i>, or <i>Neo/-</i> mice. Mean values derived from our data are color-coded according to genotype. In this study, averaged ABR threshold changes at 16 kHz were used to derive the amplification gain, although similar results were obtained at frequencies of 4–12 and 22 kHz. We assumed that the prestin amount was linearly correlated with <i>Q</i>_max, charge density or electromotility, which is further linearly correlated with feedback efficiency. Therefore, the normalized prestin activity in <i>Neo/Neo</i> and <i>Neo/-</i> OHCs by wild-type control (100% prestin activity) is expressed as the feedback efficiency (β). Values are the mean ± SEM.</p