308 research outputs found

    Geochronology and Geochemistry of the Dismembered Ophiolites in the Xingshuwa Accretionary Complex in Southeastern Inner Mongolia

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    Table S1. Zircon U-Pb data of the metagabbro (P4B4-5) and tuffaceous sandstones (TD221-1 and TD221-3) in the Xingshuwa accretionary complex. Table S2. Major and trace elements of the mafic and ultramafic rocks in the Xingshuwa accretionary complex

    Geochronology and Geochemistry of the Dismembered Ophiolites in the Xingshuwa Accretionary Complex in Southeastern Inner Mongolia

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    Supporting data for "Petrology, Geochronology and Geochemistry of the Dismembered Ophiolites in the Xingshuwa Accretionary Complex in Southeastern Inner Mongolia: Implications for the Suture Zone between the Siberian and Sino-Korean Paleoplates in the Southeastern Central Asian Orogenic Belt" Table S1. Zircon U-Pb data of the metagabbro (P4B4-5) and tuffaceous sandstones (TD221-1 and TD221-3) in the Xingshuwa accretionary complex. Table S2. Major and trace elements of the mafic and ultramafic rocks in the Xingshuwa accretionary complex

    The chromosome distribution of the breed-specific in GZP, NOPG, and EUP pig breed.

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    <p>The vertical bars indicated different pig breed with the red, green and blue color for EUP, GZP and NPOG, respectively.</p

    Number of SV detected in different pig breeds.

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    Number of SV detected in different pig breeds.</p

    Table_3_Whole-Genome Re-Sequencing of Corylus heterophylla Blank-Nut Mutants Reveals Sequence Variations in Genes Associated With Embryo Abortion.xlsx

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    Yield loss in the economically important hazelnut (Corylus spp.) occurs through the frequent formation of blank nuts. Although the condition is associated with embryo abortion, we have not yet identified the regulatory genes involved. Therefore, this study aimed to determine the genes related to embryo abortion in hazel. We performed whole-genome re-sequencing and single-nucleotide polymorphism (SNP) analysis on four mutant hazelnut trees (Empty1 to Empty4, C. heterophylla) bearing blank nuts and four wild-type trees (Full1 to Full4, C. heterophylla). A paired comparison of Empty1 vs. Full1, Empty2 vs. Full2, Empty3 vs. Full3, and Empty4 vs. Full4, along with the intersection of Empty1 to Empty4, revealed 3 081 common SNPs in the four blank-nut mutants. Of these, 215 synonymous SNPs in exonic regions were distributed across 178 candidate genes. Heterozygosity analysis showed that average homozygous and heterozygous SNP ratios were respectively 0.409 and 0.591 in the samples. According to Gene Ontology classification, candidate genes were enriched in the categories of binding, catalysis, molecular transducer, transporter, and molecular function regulator. Among these, 18 of 178 genes had homozygous SNPs in Empty1–4. Cis elements in the promoter region of starch synthase 4 (SS4) contain the RY-element, implying seed-specific expression. Starch granules were absent from Empty1–4 cotyledon cells, but abundantly present in Full1–Full4 cotyledon cells. The blank-nut phenotype has heavier nut shells. Overall, we conclude that single-nucleotide variants of Acetyl-CoA carboxylase 1 (ACC1), intracellular sodium/hydrogen exchanger 2 (NHX2), UDP-glycosyltransferase 74E2 (UGT74E2), DEFECTIVE IN MERISTEM SILENCING 3 (DMS3), DETOXIFICATION 43 (FRD3), and SS4 may induce embryo abortion, leading to blank-nut formation. Our results will benefit future research on how the gain or loss of candidate genes influences seed development. Moreover, our study provides novel prospects for seedless cultivar development.</p

    Genome-wide identification, phylogeny and expression analyses of group III WRKY genes in cotton (<i>Gossypium hirsutum</i>)

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    Cotton (Gossypium hirsutum) is an important economic crop, so it is of great significance to analyze its molecular mechanisms of resistance to Verticillium Wilt. The WRKY gene family encodes transcription factors (TFs) involved in plant resistance to biotic and abiotic stress. Here, we identified 17 group III WRKY genes in cotton based on the cotton genome project. The phylogenetic relationship and evolutionary of WRKY Group III TFs in Arabidopsis thaliana and G. hirsutum showed that all the WRKY Group III TFs were divided into two clades. Overall, Group III WRKYs with similar motif patterns showed a tendency to cluster into the same group in the phylogenetic tree. Elements related to jasmonic acid (JA) and salicylic acid (SA) regulation were identified in the promoter region of most WRKY Group III genes. The expression levels of five Group III genes were up-regulated more than two-fold and three genes were down-regulated after Verticillium dahliae Kleb. infection. Moreover, three group III GhWRKY genes with JA or SA regulatory element in the promoter region could be significantly induced by JA or SA, respectively. Subcellular localization and transcriptional activation analysis in yeast showed that Cot_14615 protein localized to the nucleus of mesophyll cells in Nicotiana tabacum and had transcription activation function. Our data on cotton group III WRKY genes can serve as a basis for further studies into their potential function in cotton resistant to Verticillium Wilt.</p
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