DataSheet_1_The Saturation Effect of Obesity on Bone Mineral Density for Older People: The NHANES 2017–2020.docx

IntroductionPrevious studies have shown that obesity has a positive effect on bone mineral density (BMD). However, excessive obesity is harmful to health, especially in older adults. In addition, it is unclear what body mass index (BMI) and waist circumference (WC) to maintain for the most beneficial BMD in older adults.MethodsMultivariate logistic regression models were used to investigate the association between BMI, WC, and femoral neck BMD using the most recent data from the 2017–2020 National Health and Nutrition Examination Survey (NHANES). Fitting smoothing curves and saturation effects analysis were also used to determine the association of nonlinear relationships between BMI, WC, and femoral neck BMD.ResultsThe analysis included a total of 2,903 adults. We discovered that BMD and WC were positively linked to femoral neck BMD. The favorable associations of BMI and WC with femoral neck BMD were maintained in all subgroup analyses stratified by sex and race, except among Mexican Americans. Furthermore, smoothing curve fitting revealed that the link between BMI and BMD was not only a linear connection, and that there was a saturation point. The BMI saturation value in the femoral neck BMD was 24.3 (kg/m2), according to the saturation effect analysis.ConclusionsIn persons over the age of 50, our research found a positive relationship between obesity and BMD, and we also found a saturation value between BMI and BMD. According to this study, maintaining BMI at a moderate level (about 24.3 kg/m2) would result in an optimal balance between BMI and BMD in adults over 50 years of age.</p

Anion Fluorine-Doped La_0.6Sr_0.4Fe_0.8Ni_0.2O_3−δ Perovskite Cathodes with Enhanced Electrocatalytic Activity for Solid Oxide Electrolysis Cell Direct CO₂ Electrolysis

As a promising and profound device for energy conversion, a solid oxide electrolysis cell (SOEC) can efficiently convert CO2 to CO, realizing chemical storage of renewable energy. However, developing active and stable cathode catalysts for the CO2 reduction reaction (CO2-RR) is critical for SOECs. Herein, to enhance the electrocatalytic performance of a La0.6Sr0.4Fe0.8Ni0.2O3−δ (LSFN) cathode catalyst for CO2-RR, fluorine doping is investigated as anion doping for O-site in the LSFN perovskite lattice. The results confirm that F-doped La0.6Sr0.4Fe0.8Ni0.2O3−δ (LSFNF0.1) has more oxygen vacancies and better CO2 adsorption ability (approaching 4 times than LSFN). The cell with LSFNF0.1 can achieve a maximum electrolysis current density of 1.93 A·cm–2 at 1.8 V at 850 °C, with a Rp of 0.275 Ω·cm2 at OCV. Meanwhile, the cell possesses good durability for more than 60 h at an electrolysis current density of 0.6 A·cm–2 without apparent degradation. Mechanistic analysis indicates that F-doping can accelerate the formation of intermediates during electrolysis, indirectly promoting the reaction of the bidentate carbonate (rate-determining step). This work shows that anion-doped LSFNF0.1 is a promising cathode for SOEC direct CO2 electrolysis and provides a potential route for the cathode catalyst development of SOECs

A Facile Low-Temperature Approach to Designing Controlled Amorphous-Based Titania Composite Photocatalysts with Excellent Noble-Metal-Free Photocatalytic Hydrogen Production

A microporous amorphous-based titania composite photocatalysts has been fabricated using a facile low-temperature (120 °C) synthetic method. Notably, we have successfully prepared the various stages of the amorphous/crystalline heterostructure by simply adjusting the pH value. The high-pH sample favors the formation of amorphous based titania composite structure. Additionally, the BET surface area of the sample increases with the increasing of the pH value, reaching a maximum of 358 m² g^–1 when the pH value is 12. Unexpectedly, the H₂ productivity of amorphous-based composite photocatalyst without noble metal co-catalyst increases significantly with the increasing pH value, which is attributed to the quickly increasing amorphous, and the highly active catalytic centers created by the synergistic effect between crystalline TiO₂ and amorphous ZnO. This study demonstrates that it is possible to improve the properties of the amorphous-based composite photocatalyst by properly modifying the synthesis conditions. The approach presented herein can be applied to the research of controlled amorphous-based composite photocatalytic systems

amino acid alignment - tree figure 2A

amino acid alignment - tree figure 2

Boosting the Electrocatalytic Activity of Pr_0.5Ba_0.5FeO_3−δ via Ni Doping in Symmetric Solid Oxide Electrolysis Cells

Symmetric solid oxide electrolysis cells (SSOECs) have garnered significant scientific interest due to their simplified cell architecture, robust operational reliability, and cost-effectiveness, for which a highly electrocatalytically active electrode is the decisive main factor. This work evaluates the electrochemical performance of Ni-doped Pr0.5Ba0.5FeO3−δ (PBF) perovskite materials, with a focus on Pr0.5Ba0.5Fe0.8Ni0.2O3−δ (PBFN). The experimental findings herein prove the exceptional electrocatalytic ability of PBFN in facilitating the oxygen evolution and carbon dioxide reduction reaction, surpassing the electrochemical performance of PBF. In addition, the PBFN symmetric cell has excellent performance for CO2 electrolysis, and the cell has a low polarization resistance value of 0.1 Ω·cm2. Moreover, it achieves an impressive current density value of 1.118 A·cm–2 under operating conditions of 2.0 V and 800 °C, which is superior to those of the PBF symmetric cell and the PBFN asymmetric cell. It also has a good structural and performance stability. These results imply a bright development prospect of PBFN as electrodes for SSOECs

Cobalt-Free Perovskite Oxide La_0.6Sr_0.4Fe_0.8Ni_0.2O_3−δ as Active and Robust Oxygen Electrode for Reversible Solid Oxide Cells

Reversible solid oxide cells have received increasing attention due to high efficiency. Cobalt-free perovskite electrode has compatible thermal expansion coefficient matching with the electrolyte and the reversible operation to inhibit the segregation of Sr. Herein a novel cobalt-free La0.6Sr0.4Fe0.8Ni0.2O3−δ perovskite is developed and investigated as oxygen electrode for reversible solid oxide cells. The electrochemical performance of La0.6Sr0.4Fe0.8Ni0.2O3−δ oxygen electrode in fuel cell mode and electrolysis mode is investigated in detail. The maximum power density of 961 mW cm–2 and polarization resistance of 0.142 Ω cm2 at 800 °C can be achieved in fuel cell mode. While the cell is operated in electrolysis mode, the current density ranges from 0.53 A cm–2 at 750 °C to 1.09 A cm–2 at 850 °C with 50 vol % absolute humidity at 1.3 V, and the hydrogen generation rate can reach up to 1348.5 mL (cm2 h)−1 with 90 vol % absolute humidity at 800 °C. The reversible solid oxide cells show excellent reversibility and stability during 144 h medium-term reversible operation. The results indicate that La0.6Sr0.4Fe0.8Ni0.2O3−δ has a bright prospect as the oxygen electrode material for reversible solid oxide cells

Ultraviolet Light-Assisted Ag@La_0.6Sr_0.4Fe_0.9Mn_0.1O₃ Nanohybrids: A Facile and Versatile Method for Preparation of Highly Stable Catalysts in Li‑O₂ Batteries

Catalysts with high oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) performances are crucial to the development of lithium-oxygen batteries (LOBs), which aim to promote Li2O2 deposition and decomposition and then avoid the active site passivation. Considering the metallic Ag possessing good electrical conductivity and excellent catalytic activity and perovskite oxides exhibiting high abundance, an easily tunable structure, and excellent intrinsically catalytic activity, we adopt the ultraviolet light to realize the deposition of Ag nanoparticles on the La0.6Sr0.4Fe0.9Mn0.1O3 (LSFM) substrate as a high-performance cathode for LOBs. The results confirm that the synergetic effect of Ag nanoparticle decoration and the La0.6Sr0.4Fe0.9Mn0.1O3 substrate could dramatically improve the electronic conductivity and ORR/OER kinetics of the nanohybrids. Ag@LSFM hybrids with abundant active sites and considerable electronic conductivity promote the deposition of a thinner discharge product film, and the corresponding LOBs deliver high discharge capacity (12 477 mAh g–1), a small gap between charge and discharge potentials (∼1.3 V), and high cycling stability (147 cycles). Overall, the study suggests that such a bifunctional catalyst is a promising candidate in LOBs

DataSheet_1_ADORA2A-AS1 Restricts Hepatocellular Carcinoma Progression via Binding HuR and Repressing FSCN1/AKT Axis.docx

BackgroundHepatocellular carcinoma (HCC) is one of the most aggressive malignancies. Increasing evidence revealed that long noncoding RNAs (lncRNAs) were frequently involved in various malignancies. Here, we explored the clinical significances, roles, and mechanisms of lncRNA ADORA2A antisense RNA 1 (ADORA2A-AS1) in HCC.MethodsThe clinical significances of ADORA2A-AS1 in HCC were analyzed using RNA sequencing (RNA-seq) data from The Cancer Genome Atlas (TCGA) project. The expressions of ADORA2A-AS1, Fascin Actin-Bundling Protein 1 (FSCN1), Matrix Metallopeptidase 2 (MMP2), and Baculoviral IAP Repeat Containing 7 (BIRC7) in HCC tissues and cells were measured by qRT-PCR. Cell Counting Kit-8 (CCK-8), 5-ethynyl-2’-deoxyuridine (EdU), caspase-3 activity assay, transwell migration and invasion assays, and xenograft growth and metastasis experiments were performed to evaluate the roles of ADORA2A-AS1 in HCC. RNA pull-down, RNA immunoprecipitation, qRT-PCR, Western blot, and RNA stability assay were performed to elucidate the mechanisms of ADORA2A-AS1 in HCC.ResultsADORA2A-AS1 was identified as an HCC-related lncRNA, whose low expression was correlated with advanced stage and poor outcome in HCC. Gain- and loss-of functional experiments demonstrated that ADORA2A-AS1 inhibited HCC cell proliferation, induced cell apoptosis, repressed cell migration and invasion, and repressed xenograft growth and metastasis in vivo. Mechanistically, ADORA2A-AS1 competitively bound HuR (Hu Antigen R), repressed the binding of HuR to FSCN1 transcript, decreased FSCN1 transcript stability, and downregulated FSCN1 expression. The expression of FSCN1 was negatively correlated with ADORA2A-AS1 in HCC tissues. Through downregulating FSCN1, ADORA2A-AS1 repressed AKT pathway activation. Functional rescue assays showed that blocking of FSCN1/AKT axis abrogated the roles of ADORA2A-AS1 in HCC.ConclusionLow-expression ADORA2A-AS1 is correlated with poor survival of HCC patients. ADORA2A-AS1 exerts tumor-suppressive roles in HCC via binding HuR and repressing FSCN1/AKT axis.</p

Presentation_1_Hepatoma Cell-Derived Extracellular Vesicles Promote Liver Cancer Metastasis by Inducing the Differentiation of Bone Marrow Stem Cells Through microRNA-181d-5p and the FAK/Src Pathway.PPTX

Bone marrow mesenchymal stem cells (BMSCs) are beneficial to repair the damaged liver. Tumor-derived extracellular vesicles (EV) are notorious in tumor metastasis. But the mechanism underlying hepatoma cell-derived EVs in BMSCs and liver cancer remains unclear. We hypothesize that hepatoma cell-derived EVs compromise the effects of BMSCs on the metastasis of liver cancer. The differentially expressed microRNAs (miRNAs) were screened. HepG2 cells were transfected with miR-181d-5p mimic or inhibitor, and the EVs were isolated and incubated with BMSCs to evaluate the differentiation of BMSCs into fibroblasts. Hepatoma cells were cultured with BMSCs conditioned medium (CM) treated with HepG2-EVs to assess the malignant behaviors of hepatoma cells. The downstream genes and pathways of miR-181d-5p were analyzed and their involvement in the effect of EVs on BMSC differentiation was verified through functional rescue experiments. The nude mice were transplanted with BMSCs-CM or BMSCs-CM treated with HepG2-EVs, and then tumor growth and metastasis in vivo were assessed. HepG2-EVs promoted fibroblastic differentiation of BMSCs, and elevated levels of α-SMA, vimentin, and collagen in BMSCs. BMSCs-CM treated with HepG2-EVs stimulated the proliferation, migration, invasion and epithelial-mesenchymal-transition (EMT) of hepatoma cells. miR-181d-5p was the most upregulated in HepG2-EVs-treated BMSCs. miR-181d-5p targeted SOCS3 to activate the FAK/Src pathway and SOCS3 overexpression inactivated the FAK/Src pathway. Reduction of miR-181d-5p in HepG2-EVs or SOCS3 overexpression reduced the differentiation of BMSCs into fibroblasts, and compromised the promoting effect of HepG2-EVs-treated BMSCs-CM on hepatoma cells. In vivo, HepG2-EVs-treated BMSCs facilitated liver cancer growth and metastasis. In conclusion, HepG2-EVs promote the differentiation of BMSCs, and promote liver cancer metastasis through the delivery of miR-181d-5p and the SOCS3/FAK/Src pathway.</p