Additional file 1 of Comparison of clinical effectiveness and subsequent fertility between hysteroscopic resection and vaginal repair in patients with cesarean scar defect: a prospective observational study

Additional file 1: Supplementary Figure 1. Schematic presentation of ultrasound measurement of the niche. a: the base directed to the posterior wall of the cervical canal b: the apex pointing to the anterior wall of the niche. Supplementary Figure 2. Hysteroscopic resection. A: Hysteroscopic view of a defect at the anterior uterine wall. B: Resection of the lower rim using a resectoscope. C: Coagulation of the niche’s surface. D: Hysteroscopic view after resection. Supplemental Table 1. Clinical characteristics of patients attempting pregnancy

An Electrochemical Sensor for Sunset Yellow Detection Based on Cu@Cu₂O‑BNPC Formed by Modified Porous Carbon

Control and detection of sunset yellow (SY) are an utmost demanding issue due to its high risk of detrimental effects on living systems caused by excessive ingestion. In this study, we reported the synthesis of Cu@Cu2O nanoparticle-decorated B and N codoped porous carbon (BNPC) and its use in developing a novel electrochemical sensor for SY. The Cu@Cu2O-BNPC catalyst was fabricated through single-step polymerization, followed by carbonization. Scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy characterization results showed that Cu@Cu2O anchored on the porous BNPC successfully. Compared with the BNPC-modified electrode, it was found that the Cu@Cu2O-BNPC-modified electrode showed superior electrocatalytic activity in both electrochemical impedance spectroscopy and cyclic voltammetry tests. The as-prepared Cu@Cu2O-BNPC catalyst directly acted as a sensor for amperometric detection of SY without further assembling, which exhibited an ultrahigh sensitivity of 0.09 μA nM–1 cm–2, a low limit of detection (2.4 nM), and a wide linear detection ranging from 10 nM to 8 μM. To further validate its possible application, the proposed method was successfully used for the determination of SY in Fanta drinks with satisfactory results

Gold-Promoted Electrodeposition of Metal Sulfides on Silicon Nanowire Photocathodes To Enhance Solar-Driven Hydrogen Evolution

Constructing composite structures is the key to breaking the dilemma of slow reaction kinetics and easy oxidation on the surface of lightly doped p-type silicon nanowire (SiNW) array photocathodes. Electrodeposition is a convenient and fast technique to prepare composite photocathodes. However, the low conductivity of SiNWs limits the application of the electrodeposition technique in constructing composite structures. Herein, SiNWs were loaded with Au nanoparticles by chemical deposition to decrease the interfacial charge transfer resistance and increase active sites for the electrodeposition. Subsequently, co-catalysts CoS, MoS2, and Ni3S2 with excellent hydrogen evolution activity were successfully composited by electrodeposition on the surface of SiNWs/Au. The obtained core–shell structures exhibited excellent photoelectrochemical hydrogen evolution activity, which was contributed by the plasma property of Au and the abundant hydrogen evolution active sites of the co-catalysts. This work provided a simple and efficient solution for the preparation of lightly doped SiNW-based composite structures by electrodeposition

Quasi-LD-Targeted and ONOO^–‑Responsive Fluorescent Probe for Investigating the Interaction of Nonalcoholic Fatty Liver with Drug-Induced Liver Injury

Hepatic lipid droplets (LDs) and peroxynitrite (ONOO–) levels are closely related to nonalcoholic fatty liver disease (NAFLD). Additionally, some drug-induced liver injury (DILI) is often associated with ONOO–. Here, we constructed and screened the quasi-LDs-targeted and ONOO–-responsive fluorescent probe MBDP-Py+ to investigate the interaction of NAFLD with DILI. By monitoring the upregulation of the ONOO– levels and the accumulation of LDs, MBDP-Py+ was more sensitive and efficient than tissue staining and serum markers detection in evaluating the early toxicity of NAFLD and diagnosing the anticancer-DILI. More importantly, the sensitive enhancement of fluorescence signals demonstrated that in different stages of NAFLD, the dominant element of liver injury was different in the NAFLD combined with DILI mice models. As the degree of NAFLD deepens, the synergistic effect of the two will lead to more serious liver damage

Quasi-LD-Targeted and ONOO^–‑Responsive Fluorescent Probe for Investigating the Interaction of Nonalcoholic Fatty Liver with Drug-Induced Liver Injury

Hepatic lipid droplets (LDs) and peroxynitrite (ONOO–) levels are closely related to nonalcoholic fatty liver disease (NAFLD). Additionally, some drug-induced liver injury (DILI) is often associated with ONOO–. Here, we constructed and screened the quasi-LDs-targeted and ONOO–-responsive fluorescent probe MBDP-Py+ to investigate the interaction of NAFLD with DILI. By monitoring the upregulation of the ONOO– levels and the accumulation of LDs, MBDP-Py+ was more sensitive and efficient than tissue staining and serum markers detection in evaluating the early toxicity of NAFLD and diagnosing the anticancer-DILI. More importantly, the sensitive enhancement of fluorescence signals demonstrated that in different stages of NAFLD, the dominant element of liver injury was different in the NAFLD combined with DILI mice models. As the degree of NAFLD deepens, the synergistic effect of the two will lead to more serious liver damage

Table_1_Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene editing analysis in cotton.xlsx

CRIPSR/Cas9 gene editing system is an effective tool for genome modification in plants. Multiple target sites are usually designed and the effective target sites are selected for editing. Upland cotton (Gossypium hirsutum L., hereafter cotton) is allotetraploid and is commonly considered as difficult and inefficient to transform, it is important to select the effective target sites that could result in the ideal transgenic plants with the CRISPR-induced mutations. In this study, Agrobacterium rhizogenes-mediated hairy root method was optimized to detect the feasibility of the target sites designed in cotton phytoene desaturase (GhPDS) gene. A. rhizogenes showed the highest hairy root induction (30%) when the bacteria were cultured until OD600 reached to 0.8. This procedure was successfully applied to induce hairy roots in the other three cultivars (TM–1, Lumian–21, Zhongmian–49) and the mutations were detected in GhPDS induced by CRISPR/Cas9 system. Different degrees of base deletions at two sgRNAs (sgRNA5 and sgRNA10) designed in GhPDS were detected in R15 hairy roots. Furthermore, we obtained an albino transgenic cotton seeding containing CRISPR/Cas9-induced gene editing mutations in sgRNA10. The hairy root transformation system established in this study is sufficient for selecting sgRNAs in cotton, providing a technical basis for functional genomics research of cotton.</p

Near-Infrared Fluorescent Probe with a Large Stokes Shift for Detection of Hydrogen Sulfide in Food Spoilage, Living Cells, and Zebrafish

Hydrogen sulfide (H2S) is a significant component of various physiological processes, and it can also cause a negative effect on foodstuffs. In this work, we designed and synthesized an NIR fluorescent turn-on responding probe (DDM-H2S) with a large Stokes shift (190 nm) for the detection of H2S. DDM-H2S exhibited high selectivity and sensitivity, obvious color changes, and a fast response time for tracing H2S. When DDM-H2S reacted with H2S, the PET process was eliminated, and the recovered ICT process and NIR fluorescence were observed. Moreover, DDM-H2S could image endogenous and exogenous H2S in living HeLa cells and zebrafish. What is more, the probe DDM-H2S could be deposited easily to test paper strips, which were able to detect the H2S gas produced during food spoilage (such as eggs, raw meat, and fishes) by the color of test paper strips changing from pink to purple. Therefore, this work provides a promising approach for monitoring H2S in complicated biological systems and practical food samples

Sensitivity analysis after each study was excluded by turns.

Sensitivity analysis after each study was excluded by turns.</p

5-alpha Reductase inhibitors and risk of male breast cancer: a systematic review and meta-analysis

Abstract Objective: To assess the relationship between 5α-reductase inhibitors (5ARIs) and the risk of male breast cancer (MBC). Material and Methods: We systematically searched Medline via PubMed, Embase and the Cochrane Library Central Register up to May 2017 to identify published articles related to 5ARIs and the risk of MBC. Results: Summary effect estimates were calculated by a random-effect model, and tests for multivariable-unadjusted pooled risk ratios (RR) and heterogeneity, as well as the sensitivity analyses were conducted to assess publication bias. All four studies were conducted in a quality assessment according to the Newcastle Ottawa Scale system. The strength of association between 5ARIs and the prevalence of MBC was evaluated by using summarized unadjusted pooled RR with a 95% confidence interval [CI]. Four studies involving 595.776 participants, mean age range from 60 to 73.2 years old, were included in a meta-analysis, which produced a summary unadjusted RR of the risk of MBC for the treatment of 5ARIs of 1.16 (95% CI 0.85-1.58, P=0.36) and the multivariable-adjusted RR is 1.03, (95% CI 0.75-1.41, p=0.86). There was no heterogeneity among included studies (I2=0%, P=0.49). Estimates of total effects were generally consistent with the sensitivity. Conclusion: We did not observe a positive association between the use of 5ARIs and MBC. The small number of breast cancer cases exposed to 5ARIs and the lack of an association in our study suggest that the development of breast cancer should not influence the prescribing of 5ARIs therapy.</div

Quasi-LD-Targeted and ONOO^–‑Responsive Fluorescent Probe for Investigating the Interaction of Nonalcoholic Fatty Liver with Drug-Induced Liver Injury

Hepatic lipid droplets (LDs) and peroxynitrite (ONOO–) levels are closely related to nonalcoholic fatty liver disease (NAFLD). Additionally, some drug-induced liver injury (DILI) is often associated with ONOO–. Here, we constructed and screened the quasi-LDs-targeted and ONOO–-responsive fluorescent probe MBDP-Py+ to investigate the interaction of NAFLD with DILI. By monitoring the upregulation of the ONOO– levels and the accumulation of LDs, MBDP-Py+ was more sensitive and efficient than tissue staining and serum markers detection in evaluating the early toxicity of NAFLD and diagnosing the anticancer-DILI. More importantly, the sensitive enhancement of fluorescence signals demonstrated that in different stages of NAFLD, the dominant element of liver injury was different in the NAFLD combined with DILI mice models. As the degree of NAFLD deepens, the synergistic effect of the two will lead to more serious liver damage