Table2_Identification of prognostic biomarkers in the CMTM family genes of human ovarian cancer through bioinformatics analysis and experimental verification.docx

Background: Ovarian cancer (OV) is one of the most common gynecological malignancies worldwide, and its immunotherapy has considerable prospects. Multiple members of the CMTM family were aberrantly expressed in human cancers and controlled key malignant biological processes and immune regulation in cancer development. However, little is known about the function of this gene family in ovarian cancer, especially in terms of immunity.Methods: GEPIA, Oncomine, HPA, Kaplan–Meier plotter, cBioPortal, GeneMANIA, and TIMER were used to analyze the differential gene expression, prognostic value, genetic alterations, and alterations in the immune microenvironment of the CMTM family in patients with ovarian cancer. Importantly, RT-qPCR was used to verify the gene expression of the CMTM family.Results: CMTM1/3/4/6/7/8 showed abnormally high expression at the mRNA and protein levels in OV tissues based on the GEPIA and HPA databases. RT-qPCR showed that CMTM1/6/8 was highly expressed in ovarian cancer cell lines. IHC verified that CMTM8 is highly expressed in ovarian cancer tissues and is closely related to Ki-67. Survival analysis showed that high expression of CMTM1/2/3/5/8 can lead to a significant reduction in overall survival and progression-free survival. There were many types of genetic alterations in the CMTM family. Also, CMTM1/2/3/6 had a certain correlation with the changes in the immune microenvironment such as immune cell infiltration and immune checkpoint expression, which may be the potential mechanism of the CMTM family in ovarian cancer. IHC verified that CMTM6 is highly expressed in ovarian cancer tissues and is closely related to PD-L1.Conclusion: This study confirmed that the CMTM family has abnormal expression in ovarian cancer and CMTM8 can be used as a biomarker for prognostic evaluation. Also, the CMTM family may be used as a potential target for immunotherapy based on the suppression of immune checkpoints.</p

2D/1A Strategy to Regulate Film Morphology for Efficient and Stable Nonfullerene Organic Solar Cells

Recently, the ternary blend method has been successfully applied to nonfullerene organic solar cells (OSCs) and enhanced the device performance by utilizing complementary optical absorption. Here we demonstrate the two polymer donors and one small-molecule acceptor (i.e., 2D/1A) strategy to finely regulate the blend film morphology in fullerene-free OSCs. One crystalline polymer donor, PffBT4T–2OD, can act as an effective morphology regulator for a benchmark blend of PTB7–Th and ITIC, leading to appropriate phase-separated morphology, suppressed charge recombination, efficient charge transport and high carrier mobility. The resulting solvent additive- and annealing-free fabricated bulk-heterojunction OSCs show the best power conversion efficiency (PCE) of 8.22% with a significant increase of fill factor compared to their binary counterparts. Importantly, such ternary OSCs when processed under ambient condition retain excellent device performance with a PCE of 7.57%, indicative of good air-stability

sj-docx-2-jtt-10.1177_1357633X241235982 - Supplemental material for Efficacy of telerehabilitation in patients with nonspecific neck pain: A meta-analysis

Supplemental material, sj-docx-2-jtt-10.1177_1357633X241235982 for Efficacy of telerehabilitation in patients with nonspecific neck pain: A meta-analysis by Hui Zou, Zhoupeng Lu, Peng Zhao, Jialin Wang, and Ruirui Wang in Journal of Telemedicine and Telecare</p

sj-docx-1-jtt-10.1177_1357633X241235982 - Supplemental material for Efficacy of telerehabilitation in patients with nonspecific neck pain: A meta-analysis

Supplemental material, sj-docx-1-jtt-10.1177_1357633X241235982 for Efficacy of telerehabilitation in patients with nonspecific neck pain: A meta-analysis by Hui Zou, Zhoupeng Lu, Peng Zhao, Jialin Wang, and Ruirui Wang in Journal of Telemedicine and Telecare</p

DataSheet1_Efficacy and safety of the Chinese herbal medicine Xiao Yao San for treating anxiety: a systematic review with meta-analysis and trial sequential analysis.docx

Introduction: The effectiveness and safety of the Chinese herbal medicine (CHM) Xiao Yao San (XYS) used for treating anxiety disorders are still unknown. Thus, we conducted this systematic review with meta-analysis and trial sequential analysis (TSA) to determine its safety and efficacy.Methods: We searched 12 databases for relevant studies from the inception of each database till 10 August 2023. We selected randomized controlled trials to compare the efficacy and safety of XYS (including XYS only and XYS + anxiolytics) to those of anxiolytics in patients with anxiety.Results: We found 14 trials with 1,256 patients in total that met the requirements for inclusion. We assessed the majority of studies (8 out of 14) as being at high risk of bias; 6 were assessed as having a moderate risk of bias. Three trials compared oral XYS to anxiolytic medication, and 11 trials compared oral XYS plus anxiolytics to anxiolytic treatment alone. The pooled results showed that the efficacy of treatment in the XYS + anxiolytics groups was significantly higher than that of the anxiolytics alone group (RR = 1.19; 95% CI: [1.13, 1.26]; p 2 = 0) and the adverse event rates in the XYS + anxiolytics groups were significantly lower than those in the anxiolytics alone group (RR = 0.44; 95% CI: [0.28, 0.82]; p = 0.001 2 = 13). The efficacy of treatment in the XYS alone groups was also significantly higher than that of the anxiolytics alone groups (RR = 5.41; 95% CI: [2.23, 13.11]; p 2 = 0). However, there was no statistical difference between the adverse events of the XYS alone group and the anxiolytics alone group, although the incidence of adverse events in the XYS alone group was lower than that in the anxiolytics alone group. The results of the TSA confirmed the above findings.Conclusion: The use of XYS combined with anxiolytics for treating anxiety was found to be safe and effective. However, although XYS alone is effective in the treatment of anxiety disorder, more large-scale research is needed to investigate adverse events.Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=350358, identifier CRD42022350358.</p

Data_Sheet_1_Does whole-body vibration training have a positive effect on balance and walking function in patients with stroke? A meta-analysis.docx

ObjectiveAfter a stroke, patients usually suffer from dysfunction, such as decreased balance ability, and abnormal walking function. Whole-body vibration training can promote muscle contraction, stimulate the proprioceptive system, enhance the muscle strength of low limbs and improve motor control ability. The study aims to evaluate the effectiveness of whole-body vibration training on the balance and walking function of patients with stroke.MethodsPubMed, CNKI, VIP, CBM, EBSCO, Embase and Web of Science were searched. According to the inclusion and exclusion criteria, randomized controlled trials on the effectiveness of whole-body vibration training on the balance and walking function of patients with stroke were collected. The search time ranged from the date of database construction to November 2022. The included trials were evaluated by the Cochrane risk-of-bias tool. The meta-analysis was performed using two software packages, consisting of RevMan 5.4 and Stata 12.2. If the results included in the literature were continuous variables, use the mean difference (MD) and 95% confidence interval (CI) for statistics.Results(1) A total of 22 randomized controlled trials (RCTs) with a total of 1089 patients were included. (2) The results of meta-analysis showed that: compared with the controls, step length (MD = 6.12, 95%CI [5.63, 6.62], p ConclusionWhole-body vibration training has a positive effect on the balance and walking function of patients with stroke. Thus, whole-body vibration training is a safe treatment method to improve the motor dysfunction of patients with stroke.Systematic review registration[http://www.crd.york.ac.uk/PROSPERO], identifier [CRD4202348263].</p

The long-term spatial-temporal trends and burden of esophageal cancer in one high-risk area: A population-registered study in Feicheng, China

<div><p>Background</p><p>Feicheng County is a high-risk area for esophageal cancer in Shandong province, China. It is important to determine the long-term spatio-temporal trends in epidemiological characteristics and the burden of esophageal cancer, especially since the implementation of the national esophageal cancer screening program for early detection and treatment in 2005.</p><p>Methods</p><p>The data collected in Feicheng County from 2001 to 2012 was extracted from the whole-population cancer registry system. The incidence, mortality, disability-adjusted life years (DALY) and changing trends in esophageal cancer according to age and sex were calculated and described.</p><p>Results</p><p>The incidence rate of esophageal cancer in Feicheng was consistently high, and increased significantly for male, but not for female from 2001 to 2012, according to the joinpoint regression analysis. The highest and lowest yearly crude incidence rates were 160.78 and 95.97 per 100000 for males, and 81.36 and 52.17 per 100000 for females. The highest and lowest crude yearly mortality rates were 122.26 and 94.40 per 100000 for males, and 60.75 and 49.35 per 100000for females. Esophageal squamous cell carcinoma was the main pathology type and the tumor location changed significantly from 2001 to 2012. Overall, the DALY remained roughly stable and was estimated as 11.50 for males and 4.90 for females per 1000 people. The burden was mainly caused by premature death. There is an obvious spatial pattern in the distribution of incidence density and burden.</p><p>Conclusion</p><p>Esophageal cancer remains a public health issue in Feicheng County with a high incidence, mortality and disease burden. The incidence and burden have obvious spatial heterogeneity, and further studies should be conducted to identify geographical risk factors for precise local prevention and control measures.</p></div

Rapidly Responsive and Highly Selective Fluorescent Probe for Bisulfite Detection in Food

The new fluorescent probe 4-hydroxy-3-((2<i>E</i>,4<i>E</i>)-5-phenylpenta-2,4-dienoyl)-2<i>H</i>-chromen-2-one (probe <b>1</b>) was designed and synthesized for selective detection of sulfite. The fluorescence intensity of the probe was decreased only in the presence of HSO₃^–; all other anions assessed resulted in an increased fluorescence response. Hence, probe <b>1</b> acts as a highly selective sensor for HSO₃^–. This sulfite sensitivity can also be readily monitored visually, as once treated with sulfite the solution shows a marked color change from yellow to colorless. Moreover, probe <b>1</b> can be conveniently used as a signal tool to determine the HSO₃^– levels in various sugar samples

The spatial distribution of the burden of disease in Feicheng County.

<p>Figures A, B, and C are the spatial distributions of the male, female and total DALY rates, respectively.</p

HE staining of the soleus muscle in the experimental groups.

<p>Representative HE stain images the control group (A), NPD group (B), LPD group (C) and LPD+KA group (D). NPD, normal protein diet; LPD, low-protein diet; LPD+KA, low-protein diet supplemented with ketoacids.</p