6 research outputs found

    A Comprehensive Analysis of miRNA/isomiR Expression with Gender Difference

    No full text
    <div><p>Although microRNAs (miRNAs) have been widely studied as epigenetic regulation molecules, fewer studies focus on the gender difference at the miRNA and isomiR expression levels. In this study, we aim to understand the potential relationships between gender difference and miRNA/isomiR expression through a comprehensive analysis of small RNA-sequencing datasets based on different human diseases and tissues. Based on specific samples from males and females, we determined that some miRNAs may be diversely expressed between different tissues and genders. Thus, these miRNAs may exhibit inconsistent and even opposite expression between males and females. According to deregulated miRNA expression profiles, some dominantly expressed miRNA loci were selected to analyze isomiR expression patterns using rates of dominant isomiRs. In some miRNA loci, isomiRs showed statistical significance between tumor and normal samples and between males and females samples, suggesting that isomiR expression patterns are not always invariable but may vary between males and females, as well as among different tissues, tumors, and normal samples. The divergence implicates the fluctuation in the expression of miRNA and its detailed expression at the isomiR levels. The divergence also indicates that gender difference may be an important factor that affects the screening of disease-associated miRNAs and isomiRs. This study suggests that miRNA/isomiR expression and gender difference may be more complex than previously assumed and should be further studied according to specific samples from males or females.</p></div

    The flowchart to analyze miRNA/isomiR in the study.

    No full text
    <p>The flowchart to analyze miRNA/isomiR in the study.</p

    Statistical analysis between paired tumor and normal samples or male and female samples using <i>t</i> test in Fig 4.

    No full text
    <p>Statistical analysis between paired tumor and normal samples or male and female samples using <i>t</i> test in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154955#pone.0154955.g004" target="_blank">Fig 4</a>.</p

    Screened deregulated miRNAs in males (n = 12) and females groups (n = 12).

    No full text
    <p>Screened deregulated miRNAs in males (n = 12) and females groups (n = 12).</p

    Expression distribution of rates of dominant isomiRs across different groups.

    No full text
    <p>The <i>F</i> statistic and <i>P</i> values are also presented using ANOVA analysis among different groups. F-nt: female-UCEC-nt; F-tn: female-UCEC-tn; M-nt: male-PRAD-nt; M-tn: male-PRAD-tn; Mix-nt-1: mixed-LUSC-nt; Mix-tn-1: mixed-LUSC-tn; Mix-nt-2: mixed-THCA-nt; Mix-tn-2: mixed-THCA-tn. The blue bar indicates the rate of the most dominant isomiR and the secondary dominant isomiR, the red bar indicates the rate of the most dominant isomiR and the third dominant isomiR, and the green bar indicated the rate of the most dominant isomiR and the fourth dominant isomiR.</p
    corecore