Th1 and Th2 cytokines in sera of mice.

<p>The levels of IFN-γ IL-2, IL-4, and IL-5 were determined in individual sera (n = 10 mice/group) by commercially available ELISA kits. The experiment was performed twice and each value shown represents the mean and SE of 20 value. * P<0.05 versus control group. ^# P<0.01 versus control group.</p

Concentrations of Th1and Th2 cytokines in spleen cell culture supernatants of mice.

<p>Spleen cells derived from the different groups at different times were tested under the same conditions. The experiment was performed twice (n = 10 mice/group) and each value shown represents the mean and SE of 20 value. P<0.05 versus control group. ^# P<0.01 versus control group.</p

Levels of anti-HBsAg antibody in sera of mice.

<p>Anti-HBsAg antibody was individually determined by a commercial enzyme-linked immunoassay. Two separate experiments (n = 10 mice/group) were performed and the data from two independent experiments were pooled and so each value shown represents the means and SE of 20 value. * P<0.05 versus control group. ^# P<0.01 versus control group.</p