A sensitive, robust and high-throughput isotope dilution LC–MS/MS method for quantifying three folate forms in serum: Supplementary data

This study aimed to establish an isotope dilution LC–MS/MS method for the determination of folic acid, 5-formyltetrahydrofolate and 5-methyltetrahydrofolate in human serum. This method was then used to quantify these three folate forms in the healthy adult population and supplement users. A stable 96- well solid-phase extraction system was used to prepare serum samples. The highly sensitive method was established using a Shimadzu LCMS-8060NX. The linearity was good in the range of 0.1–10 nmol/l for folic acid and 5-formyltetrahydrofolate and 1.0–100 nmol/l for 5-methyltetrahydrofolate. The accuracy and precision were good. The method was sensitive, robust and high-throughput and could be used for the routine clinical monitoring of these three folate forms in the Chinese population.</p

Additional file 1 of Exploration of the profile-effect relationship of Siraitia grosvenorii aqueous extracts related to their laxative effect on the basis of gray correlation analysis

Additional file 1

Tuning the crystallinity of the MnO_x catalysts to promote toluene catalytic oxidation

In this paper, the MnOx catalysts with excellent toluene oxidation performance were prepared by a simple precipitation method. The physicochemical properties of the prepared MnOx catalysts were investigated by XRD, BET, H2-TPR, O2-TPD and XPS. The obtained results revealed that the crystallinity of the prepared MnOx catalysts could be effectively regulated by changing the (NH4)2CO3/Mn(NO3)2 molar ratio, and thus affecting the oxygen vacancy concentration of the prepared MnOx catalysts. The prepared MnOx-4 catalyst with the (NH4)2CO3/Mn(NO3)2 molar ratio of 4.0 had the poor crystallinity and small grain size, which effectively promoted the oxygen defects in the MnOx catalyst to be formed. At the same time, the MnOx-4 catalyst had a large specific surface area, the highest low temperature reducibility and the largest number of oxygen vacancies and surface adsorbed oxygen species, which allowed more surface oxygen species to participate in the redox reaction, and promoted the toluene deep oxidation. Therefore, when the (NH4)2CO3/Mn(NO3)2 molar ratio was 4.0, the prepared MnOx-4 catalyst exhibited an excellent toluene catalytic oxidation performance and robust catalytic stability. What’s more, the toluene oxidation conversion on the MnOx-4 catalyst reached 99% at 230°C, and the MnOx-4 catalyst showed excellent resistance to water vapour.</p

Palladium-Catalyzed Migratory Insertion of Carbenes and C–C Cleavage of Cycloalkanecarboxamides

A palladium catalyzed reaction of cycloalkanecarboxamides and diazomalonates or bis­(phenylsulfonyl)­diazo­methane has been developed. The reaction proceeds via carbene migratory insertion and cascade C–C cleavage pathways. Cycloalkanecarboxamides with four to seven membered rings are applicable in the transformation. A series of ring opening products were prepared with moderate yields. The finding provides valuable clues for the development of new reactions involving carbene migratory insertion and the cleavage of unstrained C­(sp3)–C­(sp3) bonds

DataSheet_1_Efficacy and safety of transarterial chemoembolization combined with lenvatinib and camrelizumab in patients with BCLC-defined stage C hepatocellular carcinoma.pdf

ObjectiveTo investigate the effectiveness and safety of combining transarterial chemoembolization (TACE) with lenvatinib and camrelizumab in patients with Barcelona Clinic Liver Cancer (BCLC) stage C hepatocellular carcinoma (HCC).MethodsWe retrospectively analyzed 141 patients with BCLC stage C HCC: 57 were treated with TACE combined with lenvatinib plus camrelizumab (T + L + C), 41 were treated with TACE combined with camrelizumab (T + C), and 43 were treated with TACE (TACE). The primary outcomes were overall survival (OS) and progression-free survival (PFS), and the secondary outcomes were the objective response rate (ORR) and adverse events (AEs). Factors that affected survival were identified via Cox regression analysis.ResultsComparison of the three groups revealed a significant difference in the median overall survival (mOS), 19.8 months (95% CI 15.7–23.9) in the T + L + C combined group vs 15.7 (95% CI 13.1–18.3) months in the T + C combined group vs 9.4 (95% CI 6.2–12.5) months in the TACE group (P ConclusionsIn patients with BCLC stage C HCC, those receiving the T + L + C combination demonstrated a superior survival benefit and acceptable safety profile compared patients receiving either TACE or the T + C combination.</p

Oct-2-en-4-ynoyl-CoA as a Specific Inhibitor of Acyl-CoA Oxidase

Oct-2-en-4-ynoyl-CoA was found to be a specific inhibitor of acyl-CoA oxidase in fatty acid oxidation in peroxisomes that has no inhibitory effect on acyl-CoA dehydrogenase in mitochondria. The inhibition reaction involves a nucleophilic attack of Glu421 to the δ carbon of the inhibitor. The result indicates that acyl-CoA oxidase and acyl-CoA dehydrogenase have certain differences in active-site structure, which makes it possible to control fatty acid oxidation selectively in either mitochondria or peroxisomes with different enzyme inhibitors

Additional file 1: of Manipulation of two regulatory genes for efficient production of chromomycins in Streptomyces reseiscleroticus

Figure S1. UV and ESI-MS spectra of SEK15b (3, a), chromomycin A3 (1, b) and chromomycin A2 (2, c). Table S1. The 1H (300 MHz) and 13C NMR (75 MHz) data for SEK15b (3) (DMSO-d 6 , δ in ppm, J in Hz). Table S2. The 1H NMR (300 MHz) data for chromomycins A3 (1) and A2 (2) (CDCl3, δ in ppm, J in Hz). (DOCX 200 kb

Anti-Inflammatory Benzofuran Neolignans from the Fruits of Canarium album (Chinese Olive)

Chinese olive is a well-known fruit in China. In this study, we explored anti-inflammatory phytochemicals of Chinese olive fruit, which led to the isolation of nine pairs of new enantiomers of benzofuran neolignans (1a/1b–9a/9b). Among them, the planar structures of compounds 1–3 were undescribed previously, and the absolute configurations of all compounds were reported first. Their stereostructures were elucidated on the basis of the interpretation of NMR, HRESIMS, and electronic circular dichroism (ECD) data, as well as ECD calculation. All compounds exhibited pronounced inhibitory effects on lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 macrophages with IC50 values ranging from 6.0 to 30.2 μM. Furthermore, compounds 6a and 6b exert anti-inflammatory effects by blocking the nuclear translocation of NF-κB, thereby reducing the expression of pro-inflammatory mediators COX-2, iNOS, IL-1β, and IL-6. This study could provide some guidance and evidence to the further development and utilization of the Chinese olive