Konferencija Što činimo po pitanju transparentnosti

Additional file 2: Figure S2. PCR and phenotypic analysis of the Δcre1 strain T. reesei SDC11. a PCR analysis of T. reesei SDC11 with SP4 as control. 1 and 2 represent the fragment (upstream region and open reading frame of gene cre1) amplificated by the prime pair cre1-2426UF/cre1-1069R in T. reesei SDC11 and SP4, respectively; 3 and 4 represent the internal fragment of gene cre1 amplificated by the prime pair cre1-497F/cre1-1069R in T. reesei SDC11 and SP4, respectively. 5 and 6 represent the fragment of gene pyrG amplificated by the prime pair pyrG-UF1/pyrG-2426DR in T. reesei SDC11 and SCP11, respectively. b Southern blot analysis of the genomic DNA isolated from SP4 and SCP11, which were digested with EcoRI/HindIII. A 5.5-kb fragment is present in the parental strain SP4, and a 7.0-kb band is shown in Δcre1 + pyrG strain SCP11. c Growth of T. reesei SN1, Δcre1 + pyrG strain SCP11 and Δcre1 strain SDC11 on MM plate. d Growth of T. reesei SP4, Δcre1 + pyrG strain SCP11 and Δcre1 strain SDC11 on the MM plate containing uracil (0.1%)

Serum Cytokeratin 19 Fragment, CK19-2G2, as a Newly Identified Biomarker for Lung Cancer

<div><p>Background</p><p>CK19-2G2, a new fragment of cytokeratin 19, is a potential tumor marker for diagnosing lung cancer. The preoperative level of serum CK19-2G2 has been demonstrated to be associated with tumor metastasis and survival of breast cancer patients. This study investigated the postoperative dynamic changes in serum CK19-2G2 levels and its clinical significance in lung cancer patients.</p><p>Materials and Methods</p><p>Preoperative serum CK19-2G2 levels were measured in 630 lung cancer patients and were compared with individuals with benign pulmonary diseases (n = 134) and healthy volunteers (n = 263). In 352 cases, the patients underwent surgery. In these patients, in addition to preoperative assays, serum CK19-2G2 was also monitored at 1 week and 1 month after the operation.</p><p>Results</p><p>The preoperative baseline levels of serum CK19-2G2 was significantly higher in lung cancer patients than patients with benign diseases and healthy controls (P<0.001). The postoperative levels of CK19-2G2 declined significantly within 1 week after tumor resection. Hereafter, a further decrease was observed in the patients who underwent palliative operations, while for the patients in the radical resection group, their CK19-2G2 levels stabilized.</p><p>Conclusion</p><p>CK19-2G2 may be a candidate marker for diagnosing and monitoring a patient's response to lung cancer treatment. In addition, CK19-2G2 may be an indicator for micrometastases in lung cancer patients.</p></div

Dynamic changes in serum CK19-2G2 levels in lung cancer patients before and after surgery.

<p>The scatter represents a single value and the lines represent the mean levels of CK19-2G2 in lung cancer patients who received radical or palliative operations.</p

The mean level of CK19-2G2 for lung cancer, benign diseases and healthy controls.

<p>Abbreviation: NSCLC = non-small cell lung cancer; ADC = adenocarcinoma; SCC = squamous cell carcinoma; SCLC = small cell lung cancer.</p

Demographics and clinical characteristics of study population.

<p>Demographics and clinical characteristics of study population.</p

Serum CK19-2G2 concentration in the lung cancer group before and after operation.

<p>Note: T0 vs. T1 & T2: P<0.001; radical resection (T0) vs. palliative operation (T0): <i>P</i> = 0.617; radical resection (T1) vs. palliative operation (T1): <i>P</i><0.001; radical resection (T2) vs. palliative operation (T2): <i>P</i> = 0.090.</p

Serum CK19-2G2 concentrations in lung cancer patients, patients with benign diseases and healthy controls.

<p>The scatter represents a single value and the lines represent the mean levels of CK19-2G2 for the benign, healthy and malignant histology subtypes. Abbreviations: ADC: adenocarcinoma; SCC: squamous carcinoma; SCLC: small cell lung cancer.</p

Expression profiles of seed storage proteins and developmental- related CYP450 genes across three species.

<p>Clustering analysis of all genes in heatmaps in different columns represent the expression levels in Ft, Fea and Fes. A color scale is shown at the left top. Green color indicates lower expression, while red color indicates higher expression. (A) Expression profile of globulins-related genes. (B) Hierarchical clustering of allergen proteins. (C) Profiling of annexin proteins. (D) Identified <i>CYP450</i> genes expression profile.</p

Gene expression profiles of flavonoids biosynthesis genes.

<p>(A) Scheme of the biosynthetic pathway of flavonoids. (B) Comparison of gene expression patterns involved in flavonoid biosynthesis in Ft, Fea, and Fes between RNA-seq FPKM and qRT-PCR data (error bar indicates standard deviation). (C) Validation of differentially expressed flavonoid biosynthesis-related genes.</p