17 research outputs found

    Choroidal change in acute anterior uveitis associated with human leukocyte antigen-B27

    No full text
    <div><p>Purpose</p><p>To evaluate choroidal changes in eyes with acute anterior uveitis associated with human leukocyte antigen (HLA)-B27</p><p>Methods</p><p>In 44 patients with first-onset, unilateral, acute-onset (<1 week) anterior uveitis for which diagnostic work-ups revealed positivity only for HLA-B27, wide-field three-dimensional volumetric raster scan using swept-source optical coherence tomography was performed for both eyes. Choroidal thickness was measured by automated segmentation and thickness mapping and compared between eyes with uveitis and the fellow eyes at baseline. Choroidal thickness was compared before and after topical and/or systemic corticosteroid therapy. Relative choroidal thickening was defined as the choroidal thickness of the uveitic eye minus that of the corresponding eye and correlated with the degree of intraocular inflammation.</p><p>Results</p><p>Compared to the fellow eyes, eyes with acute anterior uveitis showed significant choroidal thickening on the subfoveal and parafoveal areas at baseline (all P <0.05). En face choroidal imaging showed dilation of large choroidal vessels on the macula. Relative choroidal thickening significantly correlated with the degree of anterior chamber inflammation at baseline (correlation coefficient = 0.341, P = 0.023). After treating inflammation, the choroid on the macula thinned significantly (from 262.1 ± 66.5 to 239.5 ± 61.0 μm in the subfoveal choroid, P<0.001).</p><p>Conclusions</p><p>Eyes with HLA-B27-associated anterior uveitis showed significant choroidal thickening at acute phase that subsequently decreased after treatment, indicating subclinical choroidal inflammation in the eyes. Choroidal thickness might indicate disease activity in acute anterior uveitis associated with HLA-B27.</p></div

    A photographic example of fundus photography and swept-source optical coherence tomography in an eye with acute anterior uveitis associated with human leukocyte antigen (HLA)-B27, covering a 12 (horizontal) × 9 (vertical)-mm area (box in left).

    No full text
    <p>Automated measurement of choroidal thickness by automated segmentation, which demarcates the outer border of the retinal pigment epithelium and the inner border of chorioscleral interface (indicated by lines), was performed using software provided by the manufacturer. A choroidal thickness map (upper right) was generated, and subfoveal choroidal thickness and parafoveal choroidal thicknesses were used for our analyses. Peripapillary thicknesses were also measured by automated segmentation and thickness mapping (lower), and the thicknesses on 4 quadrants (lower right) were used for our analyses. N = nasal; S = superior; T = temporal; I = inferior.</p

    En face choroidal imaging in an eye with acute anterior uveitis and the fellow eye.

    No full text
    <p>The choriocapillaris or Sattler’s layer shows no remarkable change between uveitic and fellow eyes or before and after the treatment in the uveitic eye. However, the Haller’s layer in the eye with active uveitis shows dilation of the large choroidal vessels on the macular area at baseline (Box 1) compared to images obtained after treatment (Box 2) or fellow eye, which is more remarkable in magnified images on the macular area (lower).</p

    Swept-source optical coherence tomography (SS-OCT) B-scan images in three representative patients with unilateral, acute, anterior uveitis.

    No full text
    <p>Compared to the fellow eyes (right), the eyes with acute anterior uveitis (left) show choroidal thickening of the macular area, which can be also determined by numerical values of choroidal thicknesses of the thickness maps (right side of each figure). Choroidal thickness decreased after topical and/or systemic corticosteroid therapy in the eyes with uveitis. In the fellow eyes, however, there were no significant changes in choroidal thickness before and after the treatment. The numbers within parentheses indicate the grades of anterior chamber inflammation.</p

    Comparison of macular and peripapillary choroidal thicknesses between eyes with acute anterior uveitis and the fellow eyes.

    No full text
    <p>There are significant differences in subfoveal and parafoveal choroidal thicknesses between the uveitic and fellow eyes. Asterisk (*) indicates statistical significance (P <0.05).</p

    Comparison of macular and peripapillary choroidal thicknesses before and after treatment of anterior uveitis.

    No full text
    <p>Subfoveal and parafoveal choroidal thickness thinned significantly following treatment (all P <0.05). Peripapillary choroidal thicknesses show significant difference only in nasal and temporal quadrants. Asterisk (*) indicates statistical significance (P <0.05).</p

    Demographic data and clinical characteristics of included patients.

    No full text
    <p>Data are presented as number of patients (%) or mean ± standard deviation.</p

    Delayed leaf senescence symptoms in the <i>drd1-6</i> mutant at later developmental stages.

    No full text
    <p>Rosette leaves of 28-day-old WT and the 34, 36, and 38-day-old <i>drd1-6</i> mutants were detached and darkened for 0, 3, 5 days. Photochemical efficiency of photosystem II (Fv/Fm) in WT and mutant leaves was examined at the indicated days. Data represent average values ± SE (n = 20) of independent experiments. Bars with the same letter are not significantly different at <i>P</i> < 0.05 by Tukey’s honestly significant difference (HSD) test.</p

    Delayed leaf senescence symptoms in the <i>ddm1-2</i> as well as in the <i>drd1-6</i> mutant.

    No full text
    <p>(A) Phenotypes of detached WT, <i>drd1-6</i>, <i>drd1-p</i> and <i>ddm1-2</i> leaves after 0, 3, and 5-d dark incubation. (B) Photochemical efficiency of photosystem II (Fv/Fm) in WT and mutant leaves in (A). Data represent average values ± SE (n = 27) of three independent experiments. Bars with the same letter are not significantly different at <i>P</i> < 0.05 by Tukey’s honestly significant difference (HSD) test.</p

    The positions of mutations and expression of <i>DRD1</i> gene.

    No full text
    <p>(A) Domain structures of DRD1 and DDM1 and positions of <i>drd1-6</i>, <i>drd1-p</i> and <i>ddm1-2</i>. Amino acid sequence change from tryptophan (W) to stop codon in helicase superfamily C-terminal (HELICc) domain in <i>drd1-6</i>. The triangle indicates the position of T-DNA insertion in <i>drd1-p</i> mutant. In case of <i>ddm1-2</i>, substitution of G to A in the splice donor site of intron 11 brings about lack of helicase superfamily C-terminal (HELICc) domain. (B) RT-PCR analysis of <i>DRD1</i> and control <i>ACTIN2</i> genes in WT and <i>drd1-p</i> mutant leaves. The <i>drd1-p</i> mutant displayed a decrease in <i>DRD1</i> expression levels compared to WT.</p
    corecore