4 research outputs found

    Annuaire du club alpin français

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    18921892-1892.Appartient à l’ensemble documentaire : PACA

    Additional file 5: Figure S5. of Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy

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    Susceptible cells are indistinguishable from untreated cells before drug treatment. Distribution of fluorescence levels in the susceptible phenotype before drug treatment (t = 0) and in untreated control cells before regrowth in LB (t = 0) in the a) tolC, b) tnaC, and c) ptsG reporter strain. The two populations are not statistically different, an unpaired t test with Welch’s correction yielding a P value of 0.07, 0.7, and 0.9, respectively. The bottom and top of the box are the first and third quartiles, the band inside the box is the median, the bottom and top whiskers represent the 10th and 90th percentiles, respectively. Data are obtained at least in biological triplicate (N = 3) for each reporter strain employed for a total of n S  = 6659 and n C  = 3076 susceptible and control cells, respectively. We did not observe any significant difference between the results obtained from different biological replica. (PNG 423 kb

    Additional file 1: of The spread of Injectate after ultrasound-guided lateral elbow injection – a cadaveric study

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    Example of a three-dimensional rendering of injectate distribution, showing no clear pattern or injection pooling or longitudinal injectate spread. (MP4 2766 kb

    High-Content and Semi-Automated Quantification of Responses to Estrogenic Chemicals Using a Novel Translucent Transgenic Zebrafish

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    Rapid embryogenesis, together with genetic similarities with mammals, and the desire to reduce mammalian testing, are major incentives for using the zebrafish model in chemical screening and testing. Transgenic zebrafish, engineered for identifying target gene expression through expression of fluorophores, have considerable potential for both high-content and high-throughput testing of chemicals for endocrine activity. Here we generated an estrogen responsive transgenic zebrafish model in a pigment-free “Casper” phenotype, facilitating identification of target tissues and quantification of these responses in whole intact fish. Using the ERE-GFP-Casper model we show chemical type and concentration dependence for green fluorescent protein (GFP) induction and both spatial and temporal responses for different environmental estrogens tested. We also developed a semiautomated (ArrayScan) imaging and image analysis system that we applied to quantify whole body fluorescence responses for a range of different estrogenic chemicals in the new transgenic zebrafish model. The zebrafish model developed provides a sensitive and highly integrative system for identifying estrogenic chemicals, their target tissues and effect concentrations for exposures in real time and across different life stages. It thus has application for chemical screening to better direct health effects analysis of environmental estrogens and for investigating the functional roles of estrogens in vertebrates
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