Normal blood cell counts in peripheral blood and normal CXCL12 levels in serum indicate proper CXCL12/CXCR4 signaling in HuCXCR4KI mice.

<p>Each symbol represents the result from an individual mouse. <b>A</b>, Flow cytometric analysis of total leukocyte (WBCs) and leukocyte subset counts in peripheral blood of HuCXCR4KI and WT control littermate mice. Cell counts were estimated with absolute counting beads, as described in the methods section. WBCs were defined as CD45⁺ blood cells. WBC subsets were defined based on cell surface marker expression, as follows: monocytes (CD45⁺CD11b⁺CD115⁺Ly6G^-), neutrophils (CD45⁺CD11b⁺CD115^-Ly6G⁺), T cells (CD45⁺CD11b^-Thy1.2⁺), B cells (CD45⁺CD11b^-CD19⁺), NK cells (CD45⁺CD11b^-CD49b⁺). <b>B</b>, Detection by ELISA of CXCL12 levels in the serum of HuCXCR4KI and WT control littermate mice; n.s. = non-significant (unpaired Student’s <i>t</i>-test).</p

Human CXCR4 knock-in design and confirmation of human CXCR4 expression in peripheral blood cells of heterozygous (HuCXCR4KI/WT) and homozygous (HuCXCR4KI) mice.

<p><b>A</b>, Schematic of genetic targeting strategy for knock-in of full length human CXCR4 coding region. <b>B</b>, Flow cytometric analysis of peripheral blood leukocytes from littermate mice with antibodies specific for human CXCR4.</p

Survey of human CXCR4 expression in TMA of human specimens of normal appearance and in corresponding tissues of homozygous HuCXCR4KI mice.

<p>Survey of human CXCR4 expression in TMA of human specimens of normal appearance and in corresponding tissues of homozygous HuCXCR4KI mice.</p

Expression of human CXCR4 mRNA is detectable in various organs of HuCXCR4KI mice.

<p>Relative expression levels of human CXCR4 mRNA in whole organs of HuCXCR4KI male mice, as detected by RT-qPCR (Taqman). Equal amounts of total RNA were retro-transcribed and the levels of mouse Rpl19 transcript were used as a normalization control.</p