Inhibition of Fructose-1,6-bisphosphatase by Aminoimidazole Carboxamide Ribotide Prevents Growth of Salmonella enterica purH Mutants on Glycerol

The enzyme fructose-1,6-bisphosphatase (FBP) is key regulatory point in gluconeogenesis. Mutants of Salmonella enterica lacking purH accumulate 5-amino-4-imidazole carboxamide ribotide (AICAR) and are unable to utilize glycerol as sole carbon and energy sources. The work described here demonstrates this lack of growth is due to inhibition of FBP by AICAR. Mutant alleles of fbp that restore growth on glycerol encode proteins resistant to inhibition by AICAR and the allosteric regulator AMP. This is the first report of biochemical characterization of substitutions causing AMP resistance in a bacterial FBP. Inhibition of FBP activity by AICAR occurs at physiologically relevant concentrations and may represent a form of regulation of gluconeogenic flux in Salmonella enterica

Wire tomography in the H-1NF heliac for investigation of fine structure of magnetic islands

Electron beam wire tomography in the H-1NF heliac enables high resolution mapping of vacuum flux surfaces with minimal disruption of the plasma operations schedule. Recent experimental results have proven this technique to be a highly accurate and high resolution method for mapping vacuum magnetic islands. Islands of width as small as delta approximately 8 mm have been measured, providing estimates of the internal rotational transform of the island. Point-to-point comparison of the mapping results with computer tracing, in conjunction with an image warping technique, enables systematic exploration of magnetic islands and surfaces of interest. Recent development of a fast mapping technique significantly reduced the mapping time and made this technique suitable for mapping at higher magnetic fields. This article presents recent experimental results and associated techniques.with support from the Australian Research Council Grant No. DP0344361

Voluntary agreements with industry

Voluntary Agreements (VAs) are becoming increasingly prominent in many countries as a policy instrument for achieving improvements in the environmental performance of industry. They aim to encourage industry led initiatives to set and meet environmental goals, to raise the environmental profile in corporate decision making, and to give participating industries the flexibility to achieve these goals in the manner which best meets their particular economic, social and political circumstances. The first section of this paper establishes an analytical framework for assessing the performance of VAs. This includes a classification of the different types of VAs and a discussion of the different criteria which could be used to evaluate their performance. The paper goes on to review the experience of several OECD countries with the implementation of VAs with energy intensive industries aimed at achieving improvements in energy efficiency or reducing the release of greenhouse gases. Three principle case studies are presented on Germany, the Netherlands and the United States with smaller studies on Canada and New Zealand. Each of the case studies sets out to describe the main characteristics of the VA, the different regulatory environments in which they are employed, and to evaluate their effectiveness in achieving environmental goals. Drawing on this information the paper goes on to identify several issues which need to be taken into consideration for the successful implementation of Vas. At present limited information is available to evaluate the success of VAs in different countries, especially in terms of economic efficiency and environmental effectiveness. This is partly because VAs have only been recently introduced in many countries and also because VAs rarely operate in isolation from other policy instruments. A significant part of this study, therefore, has focused on evaluation issues, such as how to compare performance of VAs against a "business as usual" baseline scenario or with historical trends. When such analysis has been used in this paper however, the difficulties and limitations of these methods are noted. VAs do, however, appear to play an important role in changing attitudes and awareness and generating and diffusing information. These so-called "soft effects" are difficult to quantify and often overlooked but appear to be one of the main advantages of a voluntary co-operative approach

A nonhuman primate model of inherited retinal disease.

Inherited retinal degenerations are a common cause of untreatable blindness worldwide, with retinitis pigmentosa and cone dystrophy affecting approximately 1 in 3500 and 1 in 10,000 individuals, respectively. A major limitation to the development of effective therapies is the lack of availability of animal models that fully replicate the human condition. Particularly for cone disorders, rodent, canine, and feline models with no true macula have substantive limitations. By contrast, the cone-rich macula of a nonhuman primate (NHP) closely mirrors that of the human retina. Consequently, well-defined NHP models of heritable retinal diseases, particularly cone disorders that are predictive of human conditions, are necessary to more efficiently advance new therapies for patients. We have identified 4 related NHPs at the California National Primate Research Center with visual impairment and findings from clinical ophthalmic examination, advanced retinal imaging, and electrophysiology consistent with achromatopsia. Genetic sequencing confirmed a homozygous R565Q missense mutation in the catalytic domain of PDE6C, a cone-specific phototransduction enzyme associated with achromatopsia in humans. Biochemical studies demonstrate that the mutant mRNA is translated into a stable protein that displays normal cellular localization but is unable to hydrolyze cyclic GMP (cGMP). This NHP model of a cone disorder will not only serve as a therapeutic testing ground for achromatopsia gene replacement, but also for optimization of gene editing in the macula and of cone cell replacement in general

Symmetric M-ary phase discrimination using quantum-optical probe states

We present a theoretical study of minimum error probability discrimination, using quantum- optical probe states, of M optical phase shifts situated symmetrically on the unit circle. We assume ideal lossless conditions and full freedom for implementing quantum measurements and for probe state selection, subject only to a constraint on the average energy, i.e., photon number. In particular, the probe state is allowed to have any number of signal and ancillary modes, and to be pure or mixed. Our results are based on a simple criterion that partitions the set of pure probe states into equivalence classes with the same error probability performance. Under an energy constraint, we find the explicit form of the state that minimizes the error probability. This state is an unentangled but nonclassical single-mode state. The error performance of the optimal state is compared with several standard states in quantum optics. We also show that discrimination with zero error is possible only beyond a threshold energy of (M - 1)/2. For the M = 2 case, we show that the optimum performance is readily demonstrable with current technology. While transmission loss and detector inefficiencies lead to a nonzero erasure probability, the error rate conditional on no erasure is shown to remain the same as the optimal lossless error rate.Comment: 13 pages, 10 figure

Mini-mast CSI testbed user's guide

The Mini-Mast testbed is a 20 m generic truss highly representative of future deployable trusses for space applications. It is fully instrumented for system identification and active vibrations control experiments and is used as a ground testbed at NASA-Langley. The facility has actuators and feedback sensors linked via fiber optic cables to the Advanced Real Time Simulation (ARTS) system, where user defined control laws are incorporated into generic controls software. The object of the facility is to conduct comprehensive active vibration control experiments on a dynamically realistic large space structure. A primary goal is to understand the practical effects of simplifying theoretical assumptions. This User's Guide describes the hardware and its primary components, the dynamic characteristics of the test article, the control law implementation process, and the necessary safeguards employed to protect the test article. Suggestions for a strawman controls experiment are also included

Unknowable bodies, unthinkable sexualities: lesbian and transgender legal invisibility in the Toronto women's bathhouse raid

Although litigation involving sexual orientation and gender identity discrimination claims has generated considerable public attention in recent years, lesbian and transgender bodies and sexualities still remain largely invisible in Anglo-American courts. While such invisibility is generally attributed to social norms that fail to recognize lesbian and transgender experiences, the capacity to 'not see' or 'not know' queer bodies and sexualities also involves wilful acts of ignorance. Drawing from R. v Hornick (2002) a Canadian case involving the police raid of a women's bathhouse, this article explores how lesbian and transgender bodies and sexualities are actively rendered invisible via legal knowledge practices, norms and rationalities. It argues that limited knowledge and limited thinking not only regulate the borders of visibility and belonging, but play an active part in shaping identities, governing conduct and producing subjectivity