Super resolution computational saturated absorption microscopy

Imaging beyond the diffraction limit barrier has attracted wide attention due to the ability to resolve image features that were previously hidden. Of the various super-resolution microscopy techniques available, a particularly simple method called saturated excitation microscopy (SAX) requires only a simple modification of a laser scanning microscope where the illumination beam power is sinusoidally modulated and driven into saturation. SAX images are extracted from harmonics of the modulation frequency and exhibit improved spatial resolution. Unfortunately, this elegant strategy is hindered by the incursion of shot noise that prevents high resolution imaging in many realistic scenarios. Here, we demonstrate a new technique for super resolution imaging that we call computational saturated absorption (CSA) in which a joint deconvolution is applied to a set of images with diversity in spatial frequency support among the point spread functions used in the image formation with saturated laser scanning fluorescence microscope. CSA microscopy allows access to the high spatial frequency diversity in a set of saturated effective point spread functions, while avoiding image degradation from shot noise

Media 1: Particle size limits when using optical trapping and deflection of particles for sorting using diode laser bars

Originally published in Optics Express on 14 September 2009 (oe-17-19-16731

Media 2: Particle size limits when using optical trapping and deflection of particles for sorting using diode laser bars

Originally published in Optics Express on 14 September 2009 (oe-17-19-16731

Media 3: Particle size limits when using optical trapping and deflection of particles for sorting using diode laser bars

Originally published in Optics Express on 14 September 2009 (oe-17-19-16731

Media 4: Particle size limits when using optical trapping and deflection of particles for sorting using diode laser bars

Originally published in Optics Express on 14 September 2009 (oe-17-19-16731

Media 2: Optical trapping, manipulation, and sorting of cells and colloids in microfluidic systems with diode laser bars

Originally published in Optics Express on 20 September 2004 (oe-12-19-4390

Media 3: Optical trapping, manipulation, and sorting of cells and colloids in microfluidic systems with diode laser bars

Originally published in Optics Express on 20 September 2004 (oe-12-19-4390

Media 6: Optical trapping, manipulation, and sorting of cells and colloids in microfluidic systems with diode laser bars

Originally published in Optics Express on 20 September 2004 (oe-12-19-4390

Single-Pixel Fluorescent Diffraction Tomography

Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial phase information is required to solve the inverse scattering problem. We introduce a method that extends optical diffraction tomography to imaging spatially incoherent contrast mechanisms such as fluorescent emission. Our strategy mimics the coherent scattering process with two spatially coherent illumination beams. The interferometric illumination pattern encodes spatial phase in temporal variations of the fluorescent emission, thereby allowing incoherent fluorescent emission to mimic the behavior of coherent illumination. The temporal variations permit recovery of the propagation phase, and thus the spatial distribution of incoherent fluorescent emission can be recovered with an inverse scattering model

Media 1: Optical trapping, manipulation, and sorting of cells and colloids in microfluidic systems with diode laser bars

Originally published in Optics Express on 20 September 2004 (oe-12-19-4390