Intensities of internal standard metabolites (normalized to the maximal response) analyzed by LC(RPLC)-(ESI+)- or LC(HILIC)-(ESI-)- HRMS after extraction of 50 mg or 25 mg of brain with 2.5 mL or 7 mL of MeOH/H₂O/heptane.

(PDF)</p

Intensities of internal standard metabolites (normalized to the maximal response) analyzed by LC(RPLC)-(ESI+)- or LC(HILIC)-(ESI-)- HRMS after extraction of 50 mg of brain with 2.5 mL of different proportions of MeOH/H₂O /heptane.

(PDF)</p

Intensities of internal standard metabolites (normalized to the maximal response) analyzed by LC(RPLC)-(ESI+)- or LC(HILIC)-(ESI-)- HRMS after extraction of 50 mg of liver with 2.5 mL of different proportions of MeOH/H₂O /heptane.

(PDF)</p

Intensities of internal standard metabolites (normalized to the maximal response) analyzed by LC(RPLC)-(ESI+)- or LC(HILIC)-(ESI-)- HRMS after extraction of 50 mg or 25 mg of liver with 2.5 mL or 7 mL of MeOH/H₂O/heptane.

(PDF)</p

Mass of sample (M_sample), nature and volume of extraction solvent (V_solvent), volume (V_mix) and concentration (C_mix) of IS mixture, extracted (V_extr) and reconstitution volumes for RPLC (V_RPLC) and HILIC (V_HILIC) used during the optimization of the sample preparation steps.

Mass of sample (Msample), nature and volume of extraction solvent (Vsolvent), volume (Vmix) and concentration (Cmix) of IS mixture, extracted (Vextr) and reconstitution volumes for RPLC (VRPLC) and HILIC (VHILIC) used during the optimization of the sample preparation steps.</p

Fig 7 -

Intensities of targeted metabolites detected in fish, liver, brain and gills, analyzed in (a) RPLC(ESI+) and (b) HILIC(ESI-).</p

Number of features (m/z, R_T) detected with different sample mass and volume of the extraction solvents in fish, liver, brain or gills, analyzed in LC(RPLC)-(ESI+) and LC(HILIC)-(ESI-) HRMS.

Number of features (m/z, RT) detected with different sample mass and volume of the extraction solvents in fish, liver, brain or gills, analyzed in LC(RPLC)-(ESI+) and LC(HILIC)-(ESI-) HRMS.</p

Intensities of internal standard metabolites (normalized to the maximal response) analyzed by LC(RPLC)-(ESI+)- or LC(HILIC)-(ESI-)- HRMS after extraction of 25 mg of gills with 2.5 mL of different proportions of MeOH/H₂O /heptane.

(PDF)</p

Intensities of internal standard metabolites (normalized to the maximal response) analyzed by LC(RPLC)-(ESI+)- or LC(HILIC)-(ESI-)- HRMS after extraction of 50 mg of fish with 2.5 mL of different proportions of MeOH/H₂O/heptane.

(PDF)</p

Optimized analytical workflow for the multi-matrices LC-HRMS untargeted metabolomic analysis of three-spined stickleback.

(PDF)</p