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Additional file 2. Growth and production time courses of extracellular product formation in S. cerevisiae strains PATW002, PATW011, and PATW012 in shake flask cultures. The strains were grown in shake-flasks with 50 mL SMNar (1.5 mM naringenin) and the OD600 and extracellular metabolite concentration of dihydrokaempferol (DHK), kaempferol and kaempferol 3-O-glucoside (K3G) expressed in ¾M were measured by HPLC in supernatant of cultures in time

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Additional file 5. Table with primers used in this study. Table with primers used in this study

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Additional file 3. Growth and production time courses of extracellular product formation in S. cerevisiae strains PATW066, PATW079, PATW080 and PATW076 in shake flask cultures. The strains were grown in shake-flasks with 50 mL SMG and the OD600 and extracellular metabolite concentration of coumaric and phloretic acids, naringenin, dihydrokaempferol (DHK), kaempferol and kaempferol 3-O-glucoside (K3G) expressed in ¾M were measured by HPLC in supernatant of cultures in time

MOESM7 of Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization

Additional file 7: Figure S6. More complete version of the tree tracing of Fig. 5. Tree-tracing of the genome-scale similarity across the S. cerevisiae (sub-)genomes of various Saccharomyces strains, as determined using Alpaca. The frequency at which a genome from the reference data set of 157 S. cerevisiae strains from Gallone et al [62] was identified as most similar for a sub-region of the CBS 1483 genome is depicted. The reference dataset is represented as a population tree, upon which only lineages with similarity are indicated with a thickness proportional to the frequency at which they were found as most similar. In addition to the genomes of CBS 1513, CBS 1483, CBS 7539 and CBS 1171, the tree-tracing figures of S. pastorianus Group 1 strains CBS 1503 and CBS 1538, of S. pastorianus Group 2 strains CBS 2156, WS34/70 and Hei-A, and of S. cerevisiae strains CBS 1463, A81062, CBS 6308, CBS 1487, CEN.PK113-7D and S288C are shown

MOESM6 of Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization

Additional file 6: Figure S5. A more complete version of the similarity profiles of the S. cerevisiae (sub-)genomes of various Saccharomyces strains as shown in Fig. 4. Each S. cerevisiae chromosome of the CBS 1483 assembly was partitioned in non-overlapping sub-regions of 2 Kbp. The colors represent the most similar lineages based on k-mer similarity of 157 S. cerevisiae strains from Gallone et al [62]: Asia (blue), Beer1 (green), Beer2, (gold), Mixed (orange), West-Africa (purple), Wine (red). Mosaic strains are shown in black and ambiguous or low-similarity sub-regions in white. Similarity patterns are shown for the Group 2 S. pastorianus strains CBS 1483, CBS 2156, WS34/70 and Hei-A, for the Group 1 S. pastorianus strains CBS 1503, CBS 1513 and CBS 1538, for S. cerevisiae ale-brewing strains CBS 7539, CBS 1463, A81062, CBS 1171, CBS 6308 and CBS 1483, and for S. cerevisiae laboratory strains CEN.PK113-7D and S288C

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Additional file 1. Detection of leucopelargonidin in S. cerevisiae strains IMX106-F and IMX106-FD. Leucopelargonidin is visible as a red-coloured band within the isoamyl alcohol phase after treatment with acidified butanol

MOESM3 of Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization

Additional file 3: Figure S2. The ploidy estimates for each chromosome in the CBS 1483 assembly based on Illumina short read data from this study. The red horizontal lines correspond to the median coverage of each chromosome

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Additional file 4. LC–MS chromatograms of cell pellet extracts of S. cerevisiae strains PATW076 and IMK393. The strains were grown in a bioreactor and biomass samples were taken after the reconsumption of ethanol. Pelargonidin and pelargonidin 3-O-glucoside (P3G) were found in the PATW076 sample. Chromatogram characteristics: (A) m/z range 433.111-433.115, (B) m/z range 271.055-271.065

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Additional file 8: Figure S7. The ploidy estimates for each chromosome of the S. pastorianus isolate of the Heineken A-yeast® lineage based on alignment of short-read data to the chromosome-level assembly of CBS 1483

MOESM5 of Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization

Additional file 5: Figure S4. Similarity profiles of the S. eubayanus (sub-)genomes of Group 1 and 2 S. pastorianus strains, as determined using Alpaca. Each S. eubayanus chromosome of the CBS 1483 assembly was partitioned in non-overlapping sub-regions of 2 Kbp. The colors represent the most similar lineages based on k-mer similarity of 29 S. eubayanus strains from Peris et al [63]: admixed (purple), Patagonia-A (red), Patagonia-B (blue). Similarity patterns are shown for the Group 2 strains CBS 1483, CBS 2156 and WS34/70 and the Group 1 strains CBS 1503, CBS 1513 and CBS 1538