18 research outputs found

    List of discriminatory genes in components 1 and 2 with PLS-DA analysis.

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    The tool eggNOG-mapper was used for functional annotation based on precomputed orthology assignments. (XLSX)</p

    Exploratory analyses of each dataset.

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    After processing the raw data, we checked the distribution of raw counts and performed principal component analysis. (TIF)</p

    Sample clustering.

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    The quality of the expression matrix was evaluated by sample clustering based on the distance between different samples, measured as Spearman’s correlation. No outliers were detected. (TIF)</p

    BLG-specific cytokines secreted by BLG-reactivated splenocytes from mice orally or intranasally administered with LL-wt, LL-BLG or LL-FnBPA+ BLG.

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    <p>Mice were intranasally (A) or orally (B) administered with LL-wt, LL-BLG or LL-FnBPA+ BLG then splenocytes from pooled samples were purified and reactivated with BLG. Secreted cytokines, IFN-Îł, IL-4 and IL-5 were assayed in medium. Sum of two independent experiments, 8 mice/group.</p

    BLG-specific IgG1, IgG2a and IgE in sera of mice intranasally administered with LL-wt, LL-BLG or LL-FnBPA+ BLG and then sensitized with BLG.

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    <p>BLG specific IgG1 (A), IgG2a (B) and IgE (C) were assayed in sera of mice intranasally administered or not (naive-sensitized, NS) with LL-wt, LL-BLG or LL-FnBPA+ BLG then sensitized with BLG in Alum in order to elicit a Th2 immune response. Sum of two independent experiments.* indicates P< 0.05, ANOVA and Tukey's post test.</p

    BLG-specific cytokines secreted by BLG-reactivated splenocytes from mice orally or intranasally administered with LL-wt, LL-BLG or LL-FnBPA+ BLG then BLG sensitized.

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    <p>Mice were intranasally (A) or orally (B) administered or not (NS) with LL-wt, LL-BLG or LL-FnBPA+ BLG then sensitized with BLG in Alum. Splenocytes from pooled samples were purified and reactivated with BLG. Secreted cytokines, IFN-Îł, IL-4 and IL-5 were assayed in medium. The results presented here are from one experiment representative of two performed independently, 8 mice/group.</p

    Peptidoglycan composition of cell wall from lactococci recombinant strains.

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    <p>The PG structure of the different strains LL-BLG, LL-FnBPA+ BLG and LL-mInlA+ BLG was compared. PG was extracted from each strain, digested with mutanolysin and the resulting muropeptides were separated by RP-HPLC. Absorbance was monitored at 220 nm.</p
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