Vibrio in shellfish in North Carolina

Data was generated by collecting oysters from the environment and culturing pathogenic bacteria with molecular confirmation. Methodology included in references

Limit of detection of LAMP reactions using the high throughput sample processing system.

<p>Representative amplifications experiments using serial dilutions of samples of known parasitaemia (quantified using the WHO International Standard as calibrator). Mean time to turbidity in minutes as follows: 10,000 p/μL = 17.5; 1,000 p/μL = 18.5; 100 p/μL = 19.4; 10 p/μL = 21.3; 1 p/μL = 22.9.</p

Stratified analysis of parasitaemia comparing DBS and WB HTP-LAMP against nPCR with a cut-off at 2 p/μL.

<p>Stratified analysis of parasitaemia comparing DBS and WB HTP-LAMP against nPCR with a cut-off at 2 p/μL.</p

Layout of the high throughput set-up on the bench.

<p>The layout of the high throughput set-up which includes a shaker, hot block, vacuum manifold and pressure gauge.</p

Flow chart of the study.

<p>The results of the two index tests, DBS-HTP-LAMP and WB-HTP-LAMP, against the gold standard nPCR results are summarized. Discrepancies between the gold standard and the index tests are highlighted. Abbreviation: EDTA ethylenediaminetetraacetic acid; PCR polymerase chain reaction; HTP-LAMP dried blood spot high throughput loop mediated isothermal amplification; DBS dried blood spot; WB whole blood.</p

Matched HT-LAMP, PCR, 2011 BS-LAMP and 2016 BS-LAMPHep+EDTA Results.xlsx

The excel table shows matching data sets comparing results from HTP-LAMP, PCR, Boil and Spin LAMP from 2011 and 2016

Comparison of Malaria HTP-LAMP results from 699 DBS and WB samples against the equivalent gold standard nPCR results from 2011.

<p>Comparison of Malaria HTP-LAMP results from 699 DBS and WB samples against the equivalent gold standard nPCR results from 2011.</p

The transportable hard-cover case containing the high throughput LAMP set-up.

<p>The transportable hard-cover case containing the high throughput LAMP set-up.</p

Layout of the contents of the high throughput sample processing kit on the bench.

<p>The contents of the sample processing kit which includes a LYSIS plate, PURE plate, EXTRACT plate, sample record sheet, lysis fluid, individual foam plugs and instruction manual.</p

A Novel, Open Access Method to Assess Sleep Duration Using a Wrist-Worn Accelerometer

<div><p>Wrist-worn accelerometers are increasingly being used for the assessment of physical activity in population studies, but little is known about their value for sleep assessment. We developed a novel method of assessing sleep duration using data from 4,094 Whitehall II Study (United Kingdom, 2012–2013) participants aged 60–83 who wore the accelerometer for 9 consecutive days, filled in a sleep log and reported sleep duration via questionnaire. Our sleep detection algorithm defined (nocturnal) sleep as a period of sustained inactivity, itself detected as the absence of change in arm angle greater than 5 degrees for 5 minutes or more, during a period recorded as sleep by the participant in their sleep log. The resulting estimate of sleep duration had a moderate (but similar to previous findings) agreement with questionnaire based measures for time in bed, defined as the difference between sleep onset and waking time (kappa = 0.32, 95%CI:0.29,0.34) and total sleep duration (kappa = 0.39, 0.36,0.42). This estimate was lower for time in bed for women, depressed participants, those reporting more insomnia symptoms, and on weekend days. No such group differences were found for total sleep duration. Our algorithm was validated against data from a polysomnography study on 28 persons which found a longer time window and lower angle threshold to have better sensitivity to wakefulness, while the reverse was true for sensitivity to sleep. The novelty of our method is the use of a generic algorithm that will allow comparison between studies rather than a “count” based, device specific method.</p></div