3 research outputs found

    TRAF6ΔDC mice exhibit disruption of microbiotic homeostasis localized to the small intestine.

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    <p><b>(A)</b> Pie charts represent bacterial composition categorized by phylum in the small bowl of control (WT) or TRAF6ΔDC (ΔDC) mice. <b>(B)</b> Heatmap analysis shows relative abundance of taxa as a percentage of total 16S rRNA, organized according to genus or most specific assigned taxon. Color scales reflect proportion contributed by each taxon. Total bacterial genome was isolated from small intestinal contents of control (WT) or TRAF6ΔDC (ΔDC) mice (n = 4, 20 week old littermate and co-housed group). <b>(C)</b> The copy number of total bacterial 16S rRNA bacteria from fecal or small intestine contents of control (WT) and TRAF6ΔDC (ΔDC) was measured by comparing to reference E. coli 16S rRNA plasmids. Some groups were fed by broad-spectrum antibiotic water (Abx) for last 2 weeks before collecting samples. **p < 0.001; n.s., not significant. Data were analyzed by Anova on Prism software (n = 3).</p

    Increased Tregs in GF TRAF6ΔDC lymphoid organs following antibiotic treatment.

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    <p><b>(A)</b> FACS plots gated on CD4<sup>+</sup> T cells show intracellular staining Foxp3 from each indicated organ of germ-free (GF) TRAF6ΔDC bone marrow chimera (ΔDC-BMC) at 8 weeks post-reconstitution. Broad-spectrum antibiotic water was provided to a group of TRAF6ΔDC bone marrow chimeras (ΔDC) for the last 2 weeks. <b>(B)</b> Percentage of Foxp3<sup>+</sup> CD4 T cells were determined from the indicated organs of TRAF6ΔDC bone marrow chimera (ΔDC-BMC) in germ-free (GF) or germ-free under antibiotics (GF-Abx). <b>(C)</b> Representative FACS plots show Foxp3<sup>+</sup> CD4 T cells from each indicated organ of germ-free (GF) B6 mice. Some of the mice were provided with broad-spectrum antibiotic water (GF-Abx) for last 2 weeks. Percentage of Foxp3<sup>+</sup> CD4 T cells were shown in each organs of germ-free B6 mice with (GF-Abx) or without antibiotics (GF). SP, spleen; MLN, mesenteric lymph node; SI, small intestine; COL, colon. **p < 0.01.</p
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