2 research outputs found

    Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum

    No full text
    During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this study, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR. The results suggest that C. thermocellum reduces the β-<i>O</i>-4 linkage content and increases the lignin S/G ratio. This investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme

    MOESM1 of Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure

    No full text
    Additional file 1: Figure A.1. Carbohydrate composition of initially screened Populus biomass. Figure A.2. Fermentation products of Avicel-control CBP cultures. Figure A.3. Carbohydrate content in Populus before and after repeat autoclave sterilization. Figure A.4. Lignin content in Populus before and after repeat autoclave sterilization
    corecore