Supplementary Figure 4 from Breast Cancer Cell Apoptosis with Phytoestrogens Is Dependent on an Estrogen-Deprived State

PDF file - 41K, Anti-estrogen, ICI 182 780 blocks phytoestrogen inhibited growth MCF7:5C cells were treated with E2(1nM), equilin (1nM), phytoestrogens (1microM) in presence of various concentrations of ICI 182 780. Total DNA was assessed in each well using a fluorescent DNA quantification kit.</p

Supplementary Figure 5 from Breast Cancer Cell Apoptosis with Phytoestrogens Is Dependent on an Estrogen-Deprived State

PDF file - 32K, ERalpha and ERbeta mRNA levels in MCF7:5C cells MCF7:5C cells were treated with 0.1% ethanol vehicle (control) and total RNA was isolated after 24h and reverse transcribed and ERalpha and ERbeta mRNA levels was obtained using RT-PCR.</p

Supplementary Table 1 from Breast Cancer Cell Apoptosis with Phytoestrogens Is Dependent on an Estrogen-Deprived State

PDF file - 65K, The sequence of the primers used in the study.</p

Supplementary Figure 1 from Breast Cancer Cell Apoptosis with Phytoestrogens Is Dependent on an Estrogen-Deprived State

PDF file - 135K, Chemical structures of estrogens used in the experiments.(A) Steroidal estrogens (B) phytoestrogens.</p

Supplementary Figure 2 from Breast Cancer Cell Apoptosis with Phytoestrogens Is Dependent on an Estrogen-Deprived State

PDF file - 101K, Differential apoptotic effects of E2 equilin and phytoestrogens MCF7:5C cells were treated with control or E2(1nM), equilin (1nM), phytoestrogens (1microM) for 72h and then stained with YO-PRO-1 and propidium iodide and analysed by flow cytometry.</p

Supplementary Figure 3 from Breast Cancer Cell Apoptosis with Phytoestrogens Is Dependent on an Estrogen-Deprived State

PDF file - 136K, Diverse effects of E2, equilin and phytoestrogens on cell cycle progression. Distribution of the cells through the cell-cycle phases was analyzed by flow cytometry in cells treated with E2(1nM), equilin (1nM), genistein (1?M), equol (1?M), coumestrol (1?M) or control for 24 h,48h and 72h. The percentage of the cells in each phase is calculated using the ModFit software. The y axis represents the number of cells and FL2-A represents the intensity of propidium iodide.</p