SYNTHESIS, IDENTIFICATIONAND INVITRO ANTITUMOURPRESCREENING TEST OF TRIPHENYLTINBENZOATETOWARDS A HUMAN CERVICAL CARCINOMACELL LINE, HeLa

ABSTRACT In this study, triphenyltin benzoate was synthesized first, and followed by antitumor prescreening test of the compound towards a human cervical carcinoma cell line, HeLa. Three reaction steps were employed to obtain the compound needed, i.e. 1) synthesizing of tetraphenyltin compound via insitu phenilmagnesiumbromide Grignard reaction to tin(lV)chloride, 2) synthesizing triphenyltin chloride via redistribution reaction of tetraphenyltin to tin(lV) chloride without any solvent, the reaction completed depends on the temperature, in this case a good results was achieved at temperature 220°C for 6 h, 3) finally, triphenyltin benzoate was produced through a methathetical reaction of triphenyltin chloride to an excess of sodium benzoate in ethanol. In vitro prescreening antitumour activity of the compound towards a human cervical tumour cell line, HeLa was ca"ied out following an enzyme linked immunosorbent assays (ELISA). By this method, the test ended with good promising results. This indicates by the ICsoof 170 nM which is compared wel/ to cisplatinum with 1Cso 950 nM. Keywords: redistribution reaction, methathetical reaction, eel/line, in vitro, antitumour

Virtual Laboratory and Self-Efficacy in Optimizing Problem-Solving Skills: A Case in Applied Chemistry Learning in Vocational College

The limitation of laboratory facilities in conducting practical chemistry learning activities has become one of the triggers for less optimal learning processes. Implementation of virtual laboratory simulation becomes a solution to overcome the limited equipment of practicum activities. It is possible to overcome some of the challenges that are currently being experienced in chemistry education by making effective use of virtual laboratories. The primary aim of this research is to investigate the impact of virtual laboratories and self-efficacy on the improvement of problem-solving skills among engineering students in the context of applied chemistry education. The research employs a quasi-experimental design to examine the impact of independent variables on dependent variables. Participants in this study are students enrolled in the Refrigeration and Air Conditioning Engineering and Utility Engineering Technology programs within the Mechanical Engineering Department of Bali State Polytechnic.  The study utilized data collection instruments consisting of tests assessing problem-solving skills and questionnaires measuring self-efficacy. The current study employs analysis of covariance (ANCOVA) as a statistical method to examine the hypothesis.The hypothesis testing results show that using virtual laboratories has a better effect than problem-solving skill demonstrations when it comes to electroplating learning activities. The observed correlation between self-efficacy and improved problem-solving skills does not seem to result in a significant impact

Peran Koperasi Simpan Pinjam dalam Mensejahterakan Anggota Pada KSP Duta Sejahtera

Koperasi merupakan suatu badan usaha yang berbadan hukum dan berasaskan kekeluargaan dengan mengutamakan rasa persaudaraan kepada seluruh anggotanya. Tujuan penelitian ini untuk mengetahui bagaimana peranan Koperasi Simpan Pinjam dalam meningkatkan kesejahteraan anggota pada KSP Duta Sejahtera, bagaimana kendala-kendala yang dihadapi KSP Duta Sejahtera dalam meningkatkan kesejahteraan anggota dan Untuk mengetahui bagaimana strategi yang digunakan KSP Duta Sejahtera dalam upaya meningkat kan kesejahteraan anggota. Penelitian yang dilakukan adalah penelitian kualitatif yang bersifat deskriptif. Subjek dalam penelitian ini adalah semua pengelola Koperasi Simpan Pinjam Duta Sejahtera. Informan dalam penelitian ini manajer koperasi, HRD, dan anggota/karyawan yang ada di KSP Duta Sejahtera. Penelitian ini tergolong penelitian lapangan, data dikumpulkan engan melihat apa yang nampak, menganalisis data dan menyimpulkannya.Teknik pengumpulan data yang digunakan yaitu wawancara, observasi dan dokumentasi. Hasil dari penelitian yang diperoleh menunjukkan bahwa Koperasi Simpan Pinjam Duta Sejahtera ini memberikan peranan yang sangat besar dalam mensejahterakan anggotanya, bentuk kesejahteraannya yaitu, kemudahan pelayanan yang diberikan, membantu membiayai pendidikan anak, jaminan kesehatan bagi pegawai, adanya pelatihan- pelatihan, hadirnya unit usaha koperasi. Kendala-kendalanya yaitu terbatasnya modal dalam pemberian kredit, kurangnya kesadaran peminjaman anggota dan tidak ada jaminan dalam peminjaman. Strategi yaitu dengan menurunkan bunga pinjaman, adanya penagih pinjaman dan koperasi harus lebih selektif dalam memberikan pinjaman kepada anggota untuk mengatasi resiko kredit

Efek Hipoglikemik Ekstrak Biji Buah Wali (Brucea Javanica L. Merr) Pada Tikus Wistar Yang Diinduksi Aloksan

Buah wali (Brucea javanica L. Merr) telah lama digunakan sebagai obat penyakit diabetes oleh masyarakat di daerah Sesaot. Tujuan penelitian ini adalah mengetahui efek hipoglikemik ekstrak biji buah wali terhadap kadar gula darah puasa (GDP) tikus wistar yang mengalami diabetes. Penelitian ini menggunakan metode experimental dengan rancangan acak lengkap (RAL). Terdapat delapan kelompok perlakuan yaitu kelompok kontrol positif (K+) dengan terapi glibenklamid 0.5 mg/kgBB, kelompok kontrol negatif (K-) yaitu hewan diabetes dengan pemberian cmc na 0.5%, kelompok N adalah hewan tidak diabetes dan  kelompok pemberian variasi dosis ekstrak biji buah wali (D1, D2, D3, D4, dan D5). Pembuatan hewan model diabetes dilakukan dengan cara induksi aloksan (125 mg/kgBB) secara intravena pada setiap kelompok kecuali pada kelompok N. Analisis data dilakukan dengan uji Anova dan LSD. Hasil penelitian ini menunjukkan persentase penurunan rata-rata gula darah selama 15 hari pengamatan sebesar 4% (D1), 10% (D2), 22% (D3), 6% (D4), 6% (D5), 14% (K+), 4% (K-), dan 2% pada kelompok N. Hasil uji Anova menunjukan bahwa p <0,05 yang berarti ada pengaruh pemberian ekstrak biji buah wali terhadap penurunan kadar gula darah tikus. Uji LSD menunjukkan pengaruh signifikan terjadi pada kelompok K+ dan kelompok variasi dosis ekstrak terhadap kelompok K- dengan nilai LSD p <0.05. Berdasarkan hasil penelitian ini, esktrak biji buah wali dapat menurunkan kadar gula darah puasa pada hewan uji diabetes dengan penurunan terbesar (22%) pada kelompok D3 dengan dosis 25 mg/kgB

Penerapan Metode Extreme Programming pada Rancang Bangun Sistem Analisis Sentimen Portal Berita

Berita dalam bentuk portal online di era globalisasi menjadi suatu wadah yang dapat digunakan oleh setiap individu untuk menyampaikan informasi tentang seorang individu ataupun organisasi, yang didalamnya terdapat penyampaian nilai emosional pribadi, baik itu bersifat negatif, netral, ataupun positif (atau lebih dikenal dengan sentimen). Keberadaan berita tersebut menciptakan suatu peluang untuk pengembangan sistem analisis sentimen terdapat informasi yang telah disampaikan dalam portal berita. Sistem analisis sentimen tersebut dapat dikembangan dengan dengan berbagai teknik dan layanan, salah satunya adalah dengan mengintegrasikan antara sistem dan layanan Google NLP, yang telah memiliki service untuk menentukan score sentimen dari setiap kalimat yang diberikan, serta penerapan teknik web scrapping sebagai metode untuk pengambilan data. Sistem dikembangan dengan framework Laravel dengan metode pengembangan Extreme Programming yang mendukung pengembangan sistem dalam waktu singkat. Pemilihan website sebagai base sistem dengan tujuan agar sistem bisa diakses dari berbagai device baik itu mobile maupun desktop. Keberadaan sistem analisis sentimen bisa dijadikan sebagai alternatif solusi bagi individu dan organisasi untuk melakukan analisis sentimen, sehingga mampu membantu dalam proses pengambilan keputusan maupun evaluasi kinerja.   Abstract News of online portals in the era of globalization has become a forum can be used by every individual to convey information about individual or organization, in which there’s the delivery of personal emotional value, negative, neutral or positive (or better known as sentiment). The existence of news creates an opportunity for development of a sentiment analysis system based on information that has been submitted in the news portal. The sentiment analysis system can be developed using various techniques and services, one of which’s by integrating the Google NLP system and service, which already has a service to determine the sentiment score of each given sentence, as well as the application of a web scrapping techniques as a method for data collection. The system was developed using the Laravel framework with the Extreme Programming development method which supports system development in short time. Selection of the website as the base system with aim that can be accessed from various devices, both mobile and desktop. The existence of a sentiment analysis system can be used as an alternative solution for individuals and organizations to carry out sentiment analysis, so that it can assist in the decision-making a process and performance evaluation

Utilization of Rhizosphere Earthworm Extracts to Support the Immune System

Periodontitis is an inflammatory condition caused by periodontal bacteria that are recognized by Toll-like receptors on neutrophils, macrophages, and other immune cells. As a result of this interaction, immune cells produce inflammatory molecules like cytokines and chemokines. In addition to the pathogenic effects of periodontitis, dysregulation of neutrophil activity can occur. The neutrophil acts as a double-edged sword in periodontitis, mobilizing defence mediators and tissue repair mechanisms while also causing further tissue damage. The goal of this study was to determine how Lumbricus luberus worm extract (EEW) affected the number of neutrophil cells in rats with periodontitis. A post-test design was used. Five Wistar rats, each infected with P. gingivalis, were divided into groups: a control group (no earthworm extract), an oral EEW (200 mg/kg/body weight) group, and a topical EEW gel group (20%). The number of neutrophil cells was measured on days 3, 7, 14, and 21. The study was carried out at the Udayana University Analytical Laboratory and the Veterinary Medicine Laboratory. The differences between control, oral, and topical Lumbricus rubellus extract administration on days 3 and 7 were significant (p &lt; 0.05). On days 14 and 21, there was a significant difference (p &lt; 0.05) between the control vs. oral administration groups and between the control vs. earthworm extract gel groups. However, on days 14 and 21, there was no significant difference between the oral administration and L. rubellus gel extract groups (p &gt; 0.05). The number of neutrophils was significantly lower following the oral administration of L. rubellus earthworm extracts. Keywords: Wistar periodontitis rats, Lumbricus rubellus earthworm extract, neutrophi

The Effect of Follicular-fluid Exposure of Endometriosis Cyst Patients toward the Levels of Bcl-2 and Cytochrome-C in Mice Oocyte

BACKGROUND: Endometriosis cysts adversely affect a woman’s quality of life, which causes pain and reduces fertility. The World Health Organization found that the incidence of endometriosis with infertility clinical manifestations is around 10%. AIM: The purpose of this study is to determine the presence of mice oocytes mitochondrial apoptosis exposed with endometriosis cysts follicular fluid through Bcl-2 and cytochrome C analysis. METHODS: This study was a randomized post-test only control group design conducted at Sanglah Hospital, Bali Royal Hospital in Denpasar, and the Medical Faculty Udayana University from June 2018 to April 2019. A total of 120 mice oocytes were distributed randomly into three groups, i.e., treatment Groups 1 and 2 that added by 15% endometriosis cysts follicular fluid and control group. Bcl-2 and Cytochrome C analyzed with ELISA Technique. Comparability was tested with one-way ANOVA with a significance level of p &lt; 0.05. RESULTS: Eighteen mice oocyte replicates were normally distributed and homogeneous. The Bcl-2 levels in the treatment Groups 1, and 2 and control were 627.83 ± 146.42, 634.50 ± 140.62, and 678.83 ± 152.71, respectively; p = 0.838 (not significantly different). Cytochrome C levels in the treatment Groups 1, and 2, and control, respectively, were 3147.75 ± 228.50, 3104.45 ± 211.29, and 2738.28 ± 227.45; p = 0.021 (significantly different). CONCLUSION: The effect of endometriosis cysts follicular fluid exposure on mitochondrial apoptosis is proven through Cytochrome C, whereas Bcl-2 is not proven

CHEMICAL BURN ON CHILDREN PATIENT DUE TO IATROGENIC OF CRESOPHENE DURING ROOT CANAL TREATMENT

Introduction: Chemical burns are injuries to the oral mucosa caused by the application of corrosive topical materials. Chemical trauma or chemical burns are one cause of mouth ulcers. Cutaneous and perioral skin chemical burn clinically, erythema, burning sensation, edema, desquamation, and ulcers. Case: A 6-year-old child patient complained of a burning sensation in the corner of the left lip during a treatment of root canal treatment using cresophene. Treatment: Patient was given a topical application of aloe vera extract dental gel, and was prescribed the same drug. Discussion: Cresophene is an antimicrobial agent used for infected root canal treatment. Cresophene is an agent antimicrobial class of phenol compounds, because it contains phenol, cresophene have antibacterial activity especially on group of gram positive bacteria. The clinical appearance of chemical burns depends on the severity of the tissue damage, the destructive properties, and mode of application of the causative agent. Aloe vera taken in a variety of dosage forms may be useful in accelerating the wound healing process and tend to raise the rate of success of healing and the rate of epithelialization in first and second burns