14 research outputs found

    Quantitative Analysis of Gangliosides in Bovine Milk and Colostrum-Based Dairy Products by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

    No full text
    Milk gangliosides have gained considerable attention because they participate in diverse biological processes, including neural development, pathogen binding, and activation of the immune system. Herein, we present a quantitative measurement of the gangliosides present in bovine milk and other dairy products and byproducts. Ultrahigh performance liquid chromatography separation was used for high-throughput analysis and achieved a short running time without sacrificing chromatographic resolution. Dynamic multiple reaction monitoring was conducted for 12 transitions for GM3 and 12 transitions for GD3. Transitions to sialic acid fragments (<i>m</i>/<i>z</i> 290.1) were chosen for the quantitation. There was a considerable amount of gangliosides in day 2 milk (GM3, 0.98 mg/L; GD3, 15.2 mg/L) which dramatically decreased at day 15 and day 90. GM3 and GD3 were also analyzed in pooled colostrum, colostrum cream, colostrum butter, and colostrum buttermilk. The separation and analytical approaches here proposed could be integrated into the dairy industry processing adding value to side-streams

    Seasonal concentrations of airborne total culturable (solid lines) and thermotolerant (dashed lines) fungi.

    No full text
    <p>(A) university campus, (B) apple field, and (C) rice field in the capital area of Seoul, South Korea. Asterisks indicate data above the upper limit of quantification (>46,000 CFU/m<sup>3</sup>).</p

    Fungal colony forming units (CFUs) observed on each nutrient agar plate<sup>a</sup>.

    No full text
    <p><sup>a</sup> The positive-hole corrections are not made for these raw CFU values.</p><p><sup>b</sup> Incubated at 25°C.</p><p><sup>c</sup> Incubated at 35°C.</p><p>Symbol: *, Data of 2-min air samplings; No asterisk, data of 5-min air samplings.</p><p>Fungal colony forming units (CFUs) observed on each nutrient agar plate<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138725#t001fn001" target="_blank"><sup>a</sup></a>.</p

    Fractions of the number of fungal colonies observed on RPMI plates containing each triazole concentration.

    No full text
    <p>The fractions of the number of fungal colonies observed on the RPMI plates for each triazole concentration to the number of fungal colonies observed on the SDAC plates are shown. The cumulative data for all sampling months and locations are used. Abbreviations: ITZ, itraconazole; TBZ, tebuconazole.</p

    Minimum inhibitory concentrations (MICs) of ITZ and TBZ in the airborne thermotolerant fungal isolates determined by the CLSI M38-A2 broth dilution method.

    No full text
    <p><sup>a</sup> Types of agar plates on which the strains were isolated.</p><p><sup>b</sup> Median values of at least four replicated experiments were used.</p><p><sup>c</sup> The values in brackets are the MIC ranges reported by the CLSI [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138725#pone.0138725.ref040" target="_blank">40</a>].</p><p>Abbreviations: ITZ, itraconazole; TBZ, tebuconazole; n.d., not determined due to difficulties in identification or difficulties in sporulation.</p><p>Minimum inhibitory concentrations (MICs) of ITZ and TBZ in the airborne thermotolerant fungal isolates determined by the CLSI M38-A2 broth dilution method.</p

    Combined High-Density Lipoprotein Proteomic and Glycomic Profiles in Patients at Risk for Coronary Artery Disease

    No full text
    <b>Objectives:</b> To test whether recently developed methods for comprehensive profiling of the high-density lipoprotein (HDL) glycome combined with the HDL proteome can distinguish individuals with coronary artery disease (CAD) from those without. <b>Methods:</b> Twenty subjects at risk for CAD, who underwent diagnostic coronary arteriography, were analyzed. Ten subjects had CAD, and ten did not. HDL was extracted from fasting plasma samples by ultracentrifugation, followed by shotgun proteomic, glycomic, and ganglioside analyses using LC-MS. CAD vs non-CAD subjects’ data were compared using univariate and multivariate statistics. <b>Results:</b> Principal components analysis showed a clear separation of CAD and non-CAD subjects, confirming that combined HDL proteomic and glycomic profiles distinguished at-risk subjects with atherosclerosis from those without. CAD patients had lower HDL apolipoprotein content (specifically ApoA-I, A-II, and E, <i>p</i> < 0.05), and lower serum amyloid A2 (SAA2, p = 0.020) and SAA4 (p = 0.007) but higher sialylated glycans (<i>p</i> < 0.05). <b>Conclusion:</b> Combined proteomic and glycomic profiling of isolated HDL was tested as a novel analytical approach for developing biomarkers of disease. In this pilot study we found that HDL proteome and glycome distinguished between individuals who had CAD from those who did not within a group of individuals equally at risk for heart disease

    Multiple Precursor Ion Scanning of Gangliosides and Sulfatides with a Reversed-Phase Microfluidic Chip and Quadrupole Time-of-Flight Mass Spectrometry

    No full text
    Precise profiling of polar lipids including gangliosides and sulfatides is a necessary step in understanding the diverse physiological role of these lipids. We have established an efficient method for the profiling of polar lipids using reversed-phase nano high-performance liquid chromatography microfluidic chip quadrupole time-of-flight mass spectrometry (nano-HPLC-chip Q-TOF/MS). A microfluidic chip design provides improved chromatographic performance, efficient separation, and stable nanospray while the advanced high-resolution mass spectrometer allowed for the identification of complex isobaric polar lipids such as NeuAc- and NeuGc-containing gangliosides. Lipid classes were identified based on the characteristic fragmentation product ions generated during data-dependent tandem mass spectrometry (MS/MS) experiments. Each class was monitored by a postprocessing precursor ion scan. Relatively simple quantitation and identification of intact ions was possible due to the reproducible retention times provided by the nano-HPLC chip. The method described in this paper was used to profile polar lipids from mouse brain, which was found to contain 17 gangliosides and 13 sulfatides. Types and linkages of the monosaccharides and their acetyl modifications were identified by low-energy collision-induced dissociation (CID) (40 V), and the type of sphingosine base was identified by higher energy CID (80 V). Accurate mass measurements and chromatography unveiled the degree of unsaturation and hydroxylation in the ceramide lipid tails

    Kaplan–Meier analysis according to the CMV ELISPOT response.

    No full text
    <p>Kaplan-Meier failure estimate showing that patients with high response of CMV ELISPOT at post-transplant 1 month had lower development of CMV DNAemia than patients with low response of CMV ELISPOT (<i>P</i> = 0.019). Patients were classified into high response (if the either pp65 or IE-1 antigens had spot counts more than the cut-off thresholds) and low response CMV (if level of both antigens were below the cut-off thresholds) post-KT 1 month CMV ELISPOT assay.</p
    corecore