DataSheet1_18KHT01, a Potent Anti-Obesity Polyherbal Formulation.docx

Obesity is a life-threatening metabolic disorder necessitating urgent development of safe and effective therapy. Currently, limited such therapeutic measures are available for obesity. The present study was designed to develop a novel, safe and effective herbal therapy for the management of obesity. A polyherbal formulation (18KHT01) was developed by homogeneously mixing a specific proportion of crude Quercus acutissima (acorn jelly powder), Camellia sinensis (dry leaf buds), and Geranium thunbergii (dry aerial part) along with Citrus limon (fruit juice). Synergistic antioxidant, antiadipogenic, and anti-obesity activities were evaluated by in vitro as well as in vivo studies. In vitro experiments revealed strong synergistic antioxidant and anti-adipogenic activities of 18KHT01. Molecular assessment of 18KHT01 showed significant down-regulation of vital adipogenic factors such as PPARγ, C/EBPα, aP2, SREBP-1c, FAS, and LPL. Based on the results of the preliminary toxicity study, 75 and 150 mg/kg, twice daily doses of 18KHT01 were administered to evaluate anti-obesity activity in diet-induced obese (DIO) C57BL/6J mice model. The major obesity-related parameters such as body weight, weight gain, food efficiency ratio, as well as serum lipid profile were significantly reduced by 18KHT01 with potential synergism. Also, the high-fat diet-induced insulin resistance was suggestively alleviated by the formulation, and thus ameliorated fasting blood glucose. Histological evaluation of liver and white adipose tissue revealed that the significant reduction of fat depositions and thus reduction of these tissue weights. Synergy evaluation experiments exhibited that the 18KHT01 offered strong synergism by improving efficacy and reducing the toxicity of its ingredients. Overall results evidenced the 18KHT01 as a safe and potent anti-obesity herbal therapy.</p

Dammarane-Type Glycosides from <i>Gynostemma pentaphyllum</i> and Their Effects on IL-4-Induced Eotaxin Expression in Human Bronchial Epithelial Cells

Two new dammarane-type glycosides, 2α,3β,12β,20S-tetrahydroxydammar-24-ene-3-O-[β-d-glucopyranosyl(1→4)-β-d-glucopyranosyl]-20-O-[β-d-xylopyranosyl-(1→6)-β-d-glucopyranoside] (1) and 2α,3β,12β,20S-tetrahydroxydammar-24-ene-3-O-β-d-glucopyranosyl-20-O-[β-d-6-O-acetylglucopyranosyl-(1→2)-β-d-glucopyranoside] (2), were isolated from a MeOH extract of the leaves of Gynostemma pentaphyllum. Their structures were elucidated by 1D and 2D NMR spectroscopic interpretation as well as by chemical studies. The isolated compounds showed potential inhibitory effects on eotaxin expression in BEAS-2B bronchial epithelial cells

Oleanane-Type Triterpenoids from <i>Aceriphyllum rossii</i> and Their Cytotoxic Activity

The hexane-soluble fraction of the roots of Aceriphyllum rossii was used to isolate seven new oleanane-type triterpenoids, aceriphyllic acids C−I (1−7), together with seven known triterpenoids. The structures of aceriphyllic acids C−I were determined as 3α-hydroxyolean-12-en-23,29-dioic acid (1), 3β-hydroxyolean-12-en-23,29-dioic acid (2), 3β,23-dihydroxyolean-12-en-29-oic acid (3), 3α-O-acetylolean-12-en-23,27-dioic acid (4), 3α-O-caffeoylolean-12-en-27-oic acid (5), 3α-O-acetylolean-12-en-23,29-dioic acid (6), and 3α-hydroxyolean-12-en-23-al-27-oic acid (7) by spectroscopic analyses. In the evaluation of the in vitro cytotoxicity of these compounds against the MCF-7 and LLC cancer cell lines, compounds 10 and 13 exhibited cytotoxic activity against the LLC cancer cell line with IC50 values of 7.63 and 6.56 μM, respectively