249 research outputs found
RELBET 4.0 user's guide
This manual describes the operation and use of RELBET 4.0 implemented on the Hewlett Packard model 9000. The RELBET System is an integrated collection of computer programs which support the analysis and post-flight reconstruction of vehicle to vehicle relative trajectories of two on-orbit free-flying vehicles: the Space Shuttle Orbiter and some other free-flyer. The manual serves both as a reference and as a training guide. Appendices provide experienced users with details and full explanations of program usage. The body of the manual introduces new users to the system by leading them through a step by step example of a typical production. This should equip the new user both to execute a typical production process and to understand the most significant variables in that process
Environmental forensics in seawater coupling a divinylbenzene passive sampling device and high resolution mass spectrometry for the screening of micropollutants
Targeted, suspected and non-target screening with high resolution mass spectrometry in the marine environment : ready to go?
The circadian clock in the diatom <i>Phaeodactylum tricornutum</i>
International audienceno abstrac
Physiological and transcriptomic evidence for a close coupling between chloroplast ontogeny and cell cycle progression in the pennate diatom <i>Seminavis robusta</i>
Despite the growing interest in diatom genomics, detailed time series of gene expression in relation to key cellular processes are still lacking. Here, we investigated the relationships between the cell cycle and chloroplast development in the pennate diatom Seminavis robusta. This diatom possesses two chloroplasts with a well-orchestrated developmental cycle, common to many pennate diatoms. By assessing the effects of induced cell cycle arrest with microscopy and flow cytometry, we found that division and reorganization of the chloroplasts are initiated only after S-phase progression. Next, we quantified the expression of the S. robusta FtsZ homolog to address the division status of chloroplasts during synchronized growth and monitored microscopically their dynamics in relation to nuclear division and silicon deposition. We show that chloroplasts divide and relocate during the S/G2 phase, after which a girdle band is deposited to accommodate cell growth. Synchronized cultures of two genotypes were subsequently used for a cDNA-amplified fragment length polymorphism-based genome-wide transcript profiling, in which 917 reproducibly modulated transcripts were identified. We observed that genes involved in pigment biosynthesis and coding for light-harvesting proteins were up-regulated during G2/M phase and cell separation. Light and cell cycle progression were both found to affect fucoxanthin-chlorophyll a/c-binding protein expression and accumulation of fucoxanthin cell content. Because chloroplasts elongate at the stage of cytokinesis, cell cycle-modulated photosynthetic gene expression and synthesis of pigments in concert with cell division might balance chloroplast growth, which confirms that chloroplast biogenesis in S. robusta is tightly regulated
Make way for the algorithms: symbolic actions and change in a regime of knowing
When actors deem technological change undesirable, they may act symbolically by pretending to comply while avoiding real change. In our study of the introduction of an algorithmic technology in a sales organization, we found that such symbolic conformity led unintendedly to the full implementation of the suggested technological change. To explain this surprising outcome we advance a regime-of-knowing lens that helps to analyze deep challenges happening ‘under the surface’ during the process of technology introduction. A regime of knowing guides what is worth knowing, what actions matter to acquire this knowledge, and who has the authority to make decisions around those issues. We found that both the technologists who introduced the algorithmic technology, as well as the incumbent workers whose work was affected by the change, used symbolic actions to either defend the established regime of knowing or to advocate a radical change. While the incumbent workers enacted symbolic conformity by pretending to comply with suggested changes, the technologists performed symbolic advocacy by presenting a positive side of the technological change. Ironically, because the symbolic conformity enabled and was reinforced by symbolic advocacy, reinforcing cycles of symbolic actions yielded a radical change in the sales' regime of knowing: from one focused on a deep understanding of customers via personal contact and strong relationships, to one based upon model predictions from the processing of large datasets. We discuss the theoretical implications of these findings for the introduction of technology at work and for knowing in the workplace.Cambridge Judge Business School internal gran
RELBET 4.0 programmer's manual
The RELBET 4.0 System as implemented on the Hewlett Packard model 9000 computer system is described. The manual is directed toward programmers and system maintenance personnel. It is intended to serve both as a reference and as a introductory guide to the software. The body of the manual provides an overview of major features and indicates where to look for further information. Full details are left to Appendices
Are the genera Hallea and mitragyna (Rubiaceae-coptosapelteae) pollen morpholically distinct?
FWN – Publicaties zonder aanstelling Universiteit Leide
Are the genera Hallea and mitragyna (Rubiaceae-coptosapelteae) pollen morpholically distinct?
FWN – Publicaties zonder aanstelling Universiteit Leide
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