LINC02163 regulates growth and epithelial-to-mesenchymal transition phenotype via miR-593-3p/FOXK1 axis in gastric cancer cells

<p>Recently, long non-coding RNAs (lncRNAs) were involved in promoting gastric cancer (GC) initiation and progression. In the current study, we revealed that the expression level of LINC02163 was elevated in GC cell lines and tissues. Knockdown of LINC02163 inhibited GC cells growth and invasion both <i>in vitro</i> and <i>in vivo</i>. Mechanismly, LINC02163 exerted as a ceRNA and negatively regulated miR-593-3p expression. In addition, FOXK1 was identified as a down-stream target of miR-593-3p. The miR-593-3p/FOXK1 axis mediated LINC02163’s effect on GC. To the best of our knowledge, our findings provided the first evidence that LINC02163 functioned as an oncogene in GC. LINC02163 may be a candidate prognostic biomarker and a target for new therapies in GC patients.</p

Forest plot from meta-analysis of DOR value using a random-effect or fixed-effect model for significant fibrosis.

<p>(A) Forest plot of LFI and (B) Forest plot of ER1. DOR: diagnostic odds ratio; LFI: liver fibrosis index; ER1: the elastic ratio of the liver for the intrahepatic vein; Ochi (a): the training set of the subjects in the study by Ochi et al; Ochi (b): the validating set of the subjects in the study by Ochi et al.</p

Forest plot from meta-analysis of DOR value using a random-effect or fixed-effect model for significant fibrosis.

<p>(A) Forest plot of LFI and (B) Forest plot of ER1 and (C) Forest plot of ER2. DOR: diagnostic odds ratio; LFI: liver fibrosis index; ER1: the elastic ratio of the liver for the intrahepatic vein; ER2: the elastic ratio of the liver for the intercostal muscle; Ochi (a): the training set of the subjects in the study by Ochi et al; Ochi (b): the validating set of the subjects in the study by Ochi et al.</p

Performance of Real-Time Elastography for the Staging of Hepatic Fibrosis: A Meta-Analysis

<div><p>Background</p><p>With the rapid development of real-time elastography (RTE), a variety of measuring methods have been developed for the assessment of hepatic fibrosis. We evaluated the overall performance of four methods based on RTE by performing meta-analysis of published literature.</p><p>Methods</p><p>Online journal databases and a manual search from April 2000 to April 2014 were used. Studies from different databases that meet inclusion criteria were enrolled. The statistical analysis was performed using a random-effects model and fixed-effects model for the overall effectiveness of RTE. The area under the receiver operating characteristic curve (AUROC) was calculated for various means. Fagan plot analysis was used to estimate the clinical utility of RTE, and the heterogeneity of the studies was explored with meta-regression analysis.</p><p>Results</p><p>Thirteen studies from published articles were enrolled and analyzed. The combined AUROC of the liver fibrosis index (LFI) for the evaluation of significant fibrosis (F≥2), advanced fibrosis (F≥3), and cirrhosis (F = 4) were 0.79, 0.94, and 0.85, respectively. The AUROC of the elasticity index (EI) ranged from 0.75 to 0.92 for F≥2 and 0.66 to 0.85 for F = 4. The overall AUROC of the elastic ratio of the liver for the intrahepatic venous vessels were 0.94, 0.93, and 0.96, respectively. The AUROC of the elastic ratio of the liver for the intercostal muscle in diagnosing advanced fibrosis and cirrhosis were 0.96 and 0.92, respectively. There was significant heterogeneity in the diagnostic odds ratio (DOR) for F≥2 of LFI mainly due to etiology (<i>p</i><0.01).</p><p>Conclusion</p><p>The elastic ratio of the liver for the intrahepatic vein has excellent precision in differentiating each stage of hepatic fibrosis and is recommend to be applied to the clinic.</p></div

Forest plot from meta-analysis of DOR value using a random-effect or fixed-effect model for significant fibrosis.

<p>(A) Forest plot of LFI and (B) Forest plot of ER1 and (C) Forest plot of ER2. DOR: diagnostic odds ratio; LFI: liver fibrosis index; ER1: the elastic ratio of the liver for the intrahepatic vein; ER2: the elastic ratio of the liver for the intercostal muscle; Ochi (a): the training set of the subjects in the study by Ochi et al; Ochi (b): the validating set of the subjects in the study by Ochi et al.</p

Characteristics of studies evaluating the performance of real time elastography for staging liver fibrosis.

<p>RTE, real time elastography; LFI, liver fibrosis index; ER1, the elastic ratio of the liver for the intrahepatic venous; ER2, the elastic ratio of the liver for the intercostal muscle; EI, elastic ratio; CHB, chronic hepatitis B; CHC, chronic hepatitis C; ALD, alcoholic liver disease, NAFLD, nonalcoholic liver fatty disease; AIH, autoimmune hepatitis; PBC, primary biliary cirrhosis.</p><p>Characteristics of studies evaluating the performance of real time elastography for staging liver fibrosis.</p

Flow diagram of search results and study selection.

<p>Flow diagram of search results and study selection.</p