MOESM1 of Overexpression of a type III PKS gene affording novel violapyrones with enhanced anti-influenza A virus activity

Additional file 1: Table S1. Plasmids and strains used in this study. Table S2. Primer pairs used in this study. Table S3. Homologous locus of vioAB in different Streptomyces genomes. Figure S1. Relative yields for compounds 1–14 in different strains. Figure S2. Spectral data of 1. Figure S3. Spectral data of 2. Figure S4. Spectral data of 3. Figure S5. Spectral data of 4. Figure S6. Spectral data of 5. Figure S7. Spectral data of 6. Figure S8. Spectral data of 7. Figure S9. Spectral data of 8. Figure S10. Spectral data of 9. Figure S11. Spectral data of 10. Figure S12. Spectral data of 11. Figure S13. Spectral data of 12. Figure S14. Spectral data of 13. Figure S15. Spectral data of 14. Figure S16. Multiple-sequence alignments of VioA with selected type III PKSs. Figure S17. Site-directed mutagenesis study of VioA

MOESM1 of Activation of a plasmid-situated type III PKS gene cluster by deletion of a wbl gene in deepsea-derived Streptomyces somaliensis SCSIO ZH66

Additional file 1: Table S1. Anti-MRSA activities of violapyrones (VLPs 1–5). Table S2. Bacteria and plasmids used in this study. Table S3. The primer pairs used for cosmid library screening. Table S4. The primer pairs used for PCR-targeted mutagenesis. Table S5. The primer pairs used for PCR confirmation of the mutants. Table S6. The primer pairs used for qPCR analysis. Figure S1. Inactivation of wblA so . Figure S2. Spectral data of VLP B, 1. Figure S3. Inactivation of pksIII-1. Figure S4. Inactivation of vioA. Figure S5. Inactivation of vioB. Figure S6. Inactivation of orf1. Figure S7. Inactivation of orf(-1-2). Figure S8. Spectral data of VLP J, 3. Figure S9. Spectral data of VLP A, 2. Figure S10. Spectral data of VLP C, 4. Figure S11. Spectral data of VLP H, 5. Figure S12. Phenotypes of the S. somaliensis SCSIO ZH66 strains

Chemical constituents from <i>Swertia mussotii</i> Franch. (Gentianaceae)

<p>The chemical investigation of ethanolic extract from <i>Swertia mussotii</i> Franch. has resulted in the isolation of 11 compounds which were identified as Orcinol-β-D-glucoside (<b>1</b>), Shamimin (<b>2</b>), Mangiferin (<b>3</b>), Decussatin (<b>4</b>), Bellidifolin (<b>5</b>), Desmethylbellidifolin (<b>6</b>), Protocatechuic acid (<b>7</b>), 1,7-Dihydroxy-3,8-dimethoxyxanthone (<b>8</b>), 1,8-Dihydroxy-3,5-dimethoxyxanthone (<b>9</b>), 1-Hydroxy-3,5-dimethoxyxanthone (<b>10</b>), Telephioidin (<b>11</b>). The chemical structures of these compounds were identified by a combination of spectroscopic analysis and a comparison with those reported in literature. Among them, compounds <b>1</b>, <b>2</b>, <b>7</b> and <b>11</b> were isolated from the genus <i>Swertia</i> for the first time. Moreover, the chemotaxonomic significance of these compounds was summarised. The chemotaxonomic study suggests that there is a close chemotaxonomic relationship between <i>S. mussotii</i> and other species of <i>Swertia</i>, such as <i>S. punicea</i>, <i>S. macrosperma</i>, <i>S. japonica</i>, <i>S. phragmitiphylla</i>, <i>S. chirayita</i>, <i>S. cordata</i> and <i>S. binchuanensis</i>, with presence of compounds <b>3</b>~<b>6</b>, <b>8</b>~<b>10</b>. The xanthones and their glycosides may sever as important chemotaxonomic markers of <i>Swertia</i> genus.</p

NMR-Metabolomic Profiling and Genome Mining Drive the Discovery of Cyclic Decapeptides from a Marine <i>Streptomyces</i>

The integration of NMR-metabolomic and genomic analyses can provide enhanced identification of structural properties as well as key biosynthetic information, thus achieving the targeted discovery of new natural products. For this purpose, NMR-based metabolomic profiling of the marine-derived Streptomyces sp. S063 (CGMCC 14582) was performed, by which N-methylated peptides possessing unusual negative 1H NMR chemical shift values were tracked. Meanwhile, genome mining of this strain revealed the presence of an unknown NRPS gene cluster (len) with piperazic-acid-encoding genes (lenE and lenF). Under the guidance of the combined information, two cyclic decapeptides, lenziamides D1 (1) and B1 (2), were isolated from Streptomyces sp. S063, which contains piperazic acids with negative 1H NMR values. The structures of 1 and 2 were determined by extensive spectroscopic analysis combined with Marfey’s method and ECD calculations. Furthermore, we provided a detailed model of lenziamide (1 and 2) biosynthesis in Streptomyces sp. S063. In the cytotoxicity evaluation, 1 and 2 showed moderate growth inhibition against the human cancer cells HEL, H1975, H1299, and drug-resistant A549–taxol with IC50 values of 8–24 μM

Iridoid compounds from the aerial parts of <i>Swertia mussotii</i> Franch. with cytotoxic activity

One new secoiridoid compound swertiamarin B (1), along with a known compound lytanthosalin (2), were isolated from ethanol extract of the aerial parts of Swertia mussotii. Their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. All compounds were first isolated from the Swertia genus. Their antitumor activities were evaluated for four human tumor cell lines (HCT-116, HepG2, MGC-803 and A549). Compounds 1 and 2 showed excellent cytotoxic activities toward the MGC-803 cell lines with IC50 values 3.61 and 12.04 μM, respectively.</p

Table_1_Comparison of 10 obesity-related indices for predicting hypertension based on ROC analysis in Chinese adults.DOCX

ObjectiveTo compare the predictive performance of the percentage body fat (PBF), body mass index (BMI), waist circumference (WC), hip circumference (HC), waist–hip ratio (WHR), waist–height ratio (WHtR), a body shape index (ABSI), body roundness index (BRI), abdominal volume index (AVI), and conicity index (CI) for identifying hypertension.MethodsA cross-sectional study was conducted among 2,801 adults (1,499 men and 1,302 women) aged 18 to 81 in Ningbo, China. The receiver operator characteristic (ROC) analysis and multiple non-parametric Z tests were used to compare the areas under the curve (AUC). The maximum Youden's indices were used to determine the optimal cut-off points of 10 obesity-related indices (ORI) for hypertension risk.ResultsThe AUC of all the indices were statistically significant (P 2, 23.46 kg/m2), HC (97.59 cm, 94.82 cm), WC (90.26 cm, 82.78 cm), WHR (0.91, 0.88), WHtR (0.51, 0.55), ABSI (0.08 m7/6/kg2/3, 0.08 m7/6/kg2/3), BRI (4.05, 4.32), AVI (16.31 cm2, 13.83 cm2), and CI (1.23 m2/3/kg1/2, 1.27 m2/3/kg1/2). Multivariate logistic regression models showed that all indices were statistically significant (P ConclusionsAll 10 ORI (PBF, BMI, HC, WC, WHR, WHtR, ABSI, BRI, AVI, and CI) can effectively predict hypertension, among which WHR should be recommended as the best predictor. Central ORI (WHR, WC, and AVI) had a better predictive performance than general ORIs (PBF and BMI) when predicting the risk of hypertension.</p

Alashanines A–C, Three Quinone-Terpenoid Alkaloids from <i>Syringa pinnatifolia</i> with Cytotoxic Potential by Activation of ERK

Three quinone-terpenoid alkaloids, alashanines A–C (1–3), possessing an unprecedented 6/6/6 tricyclic conjugated backbone and quinone–quinoline-fused characteristic, were isolated from the peeled stems of Syringa pinnatifolia. Their structures were elucidated by analysis of extensive spectroscopic data and quantum chemical calculations. A hypothesis of biosynthesis pathways for 1–3 was proposed on the basis of the potential precursor iridoid and benzoquinone. Compound 1 exhibited antibacterial activities against Bacillus subtilis and cytotoxicity against HepG2 and MCF-7 human cancer cell lines. The results of the cytotoxic mechanism revealed that compound 1 induced apoptosis of HepG2 cells through activation of ERK

Alashanines A–C, Three Quinone-Terpenoid Alkaloids from <i>Syringa pinnatifolia</i> with Cytotoxic Potential by Activation of ERK

Three quinone-terpenoid alkaloids, alashanines A–C (1–3), possessing an unprecedented 6/6/6 tricyclic conjugated backbone and quinone–quinoline-fused characteristic, were isolated from the peeled stems of Syringa pinnatifolia. Their structures were elucidated by analysis of extensive spectroscopic data and quantum chemical calculations. A hypothesis of biosynthesis pathways for 1–3 was proposed on the basis of the potential precursor iridoid and benzoquinone. Compound 1 exhibited antibacterial activities against Bacillus subtilis and cytotoxicity against HepG2 and MCF-7 human cancer cell lines. The results of the cytotoxic mechanism revealed that compound 1 induced apoptosis of HepG2 cells through activation of ERK

Charge-Carrier Dynamics of Solution-Processed Antimony- and Bismuth-Based Chalcogenide Thin Films

Chalcogenide-based semiconductors have recently emerged as promising candidates for optoelectronic devices, benefiting from their low-cost, solution processability, excellent stability and tunable optoelectronic properties. However, the understanding of their fundamental optoelectronic properties is far behind the success of device performance and starts to limit their further development. To fill this gap, we conduct a comparative study of chalcogenide absorbers across a wide material space, in order to assess their suitability for different types of applications. We utilize optical-pump terahertz-probe spectroscopy and time-resolved microwave conductivity techniques to fully analyze their charge-carrier dynamics. We show that antimony-based chalcogenide thin films exhibit relatively low charge-carrier mobilities and short lifetimes, compared with bismuth-based chalcogenides. In particular, AgBiS2 thin films possess the highest mobility, and Sb2S3 thin films have less energetic disorder, which are beneficial for photovoltaic devices. On the contrary, Bi2S3 showed ultralong carrier lifetime and high photoconductive gain, which is beneficial for designing photoconductors