Thymic Stromal Lymphopoietin Neutralization Inhibits the Immune Adjuvant Effect of Di-(2-Ethylhexyl) Phthalate in Balb/c Mouse Asthma Model - Fig 8

<p><b>Increased levels of TSLP in BALF (A) and lung homogenates (B).</b> Values were mean ± SEM. * <i>p</i><0.05 and ** <i>p</i><0.01, significant difference compared with the saline group; # <i>p</i><0.05 and ## <i>p</i><0.01, significant difference compared with the OVA group; & <i>p</i><0.05 and && <i>p</i><0.01, significant difference.</p

Absorbance of anti-DBP antibody bound to hapten-coated polystyrene wells.

<p>Absorbance at 492 nm under different concentrations of EDC and conjugation temperatures. Each data represents the mean of ±SD of three replicates (three plates on the same day).</p

Association between the DEHP-induced adjuvant effect and changes in Ig levels in serum.

<p>(A) The content of Total-IgE in serum. (B) The content of OVA-sIgE in serum. (C) The content of OVA-sIgG1 in serum. Group data were expressed as mean±SEM. **, <i>p</i><0.01 significant difference compared with the control, *, <i>p</i><0.05, significant difference compared with the control, and ##, <i>p</i><0.01, significant difference when comparing with the DEHP+OVA group and the anti-TSLP mAb group.</p

Correlation of ELISA and GC-MS analysis of certain household items.

<p>Each point represents the mean of three replicates of one sample.</p

Influence of organic solvents in assay buffer on parameters of icELISA.

<p>Each data represents the mean of ±SD of three replicates (three plates on the same day).</p

Scheme of one possible reaction in permanganate oxidation of polystyrene and its subsequent reaction with DBAP.

<p>Scheme of one possible reaction in permanganate oxidation of polystyrene and its subsequent reaction with DBAP.</p

Increased levels of ECP in lung homogenates.

<p>All data were expressed as mean ± SEM. * <i>p</i><0.05 and ** <i>p</i><0.01, compared with the saline control; #, <i>p</i><0.05, compared with the OVA group, &&, <i>p</i><0.01, significant difference between DEHP+OVA group and anti-TSLP group.</p

Cross-reactivity of some compounds structurally related to DBP by hapten coated icELISA.

<p><i>^a</i>Each data represents the mean of ±SD of three replicates (three plates on the same day).</p

Increased AHR promoted by the DEHP-adjuvant effect.

<p>(A) inspiratory resistance (Ri), (B) expiratory resistance (Re) and (C) lung dynamic compliance (Cldyn). Group data were expressed as mean ± SEM. * <i>p</i><0.05 and ** <i>p</i><0.01, significant difference compared with the saline group; # <i>p</i><0.05 and ## <i>p</i><0.01, significant difference compared with the OVA group; & <i>p</i><0.05 and && <i>p</i><0.01, significant difference between DEHP+OVA group and anti-TSLP group.</p

Sensitization and challenge protocol for an ovalbumin-induced mouse asthma model.

<p>Sensitization and challenge protocol for an ovalbumin-induced mouse asthma model.</p