2 research outputs found
Monitoring Dopamine Quinone-Induced Dopaminergic Neurotoxicity Using Dopamine Functionalized Quantum Dots
Dopamine (DA) quinone-induced dopaminergic
neurotoxicity is known to occur due to the interaction between DA
quinone and cysteine (Cys) residue, and it may play an important a
role in pathological processes associated with neurodegeneration.
In this study, we monitored the interaction process of DA to form
DA quinone and the subsequent Cys residue using dopamine functionalized
quantum dots (QDs). The fluorescence (FL) of the QD bioconjugates
changes as a function of the structure transformation during the interaction
process, providing a potential FL tool for monitoring dopaminergic
neurotoxicity
Target-Specific Imaging of Transmembrane Receptors Using Quinonyl Glycosides Functionalized Quantum Dots
Here, we describe a novel “switch-on”
biosensor based
on quinonyl glycosides functionalized quantum dots (QDs) for the specific
targeting and imaging of transmembrane glycoprotein receptors on the
surface of cancer cells. The design of the quinonyl glycosides lies
in that the quinone moiety serves as a quencher of QDs and the glycoside
moiety as a biospecific ligand for targeting a receptor. We observed
that the quenched photoluminescence of the quinone glycosides functionalized
QDs could be significantly recovered by a specific lectin that selectively
binds to the glycosides clustering the QDs but was not affected by
a panel of nonspecific lectins. Moreover, we determined that quinonyl
galactoside functionalized QDs could optically image the asialoglycoprotein
receptors of a hepatoma cell line in a target-specific manner. This
system might provide new insights into the fabrication of photoluminogenic
biosensors for the analysis of the universal ligand–receptor
recognitions in nature