1 research outputs found
Schematic drawing of the luciferase reporter vectors (psi-CHECK2) contains <i>NBS1</i> 3’-UTR sequence with either the G or C at the rs2735383 locus.
<p>The luciferase activity of the rs2735383 variant allele on luciferase reporter bearing <i>NBS1</i> 3’-UTR co-transfected with or without hsa-miR-509-5p mimics or hsa-miR-509-5p inhibitors in HCT116 cells <b>(A)</b> and HT-29 cells <b>(B)</b>. Renilla luciferase activity was measured and normalized to firefly luciferase. Six replicates were carried out for each group, and the experiment was repeated at least three times. Data are mean±standard error of the mean; **<i>P</i><0.01. <b>(C)</b><i>NBS1</i> mRNA expression level in tumor tissue samples from colorectal cancer individuals with different rs2735383 variant alleles genotype (11 rs2735383GG, 13 rs2735383GC and 5 rs2735383CC); data are mean±standard error of the mean, normalized to <i>GAPDH</i>, <i>P</i> = 0.027. <b>(D)</b> hsa-miR-509-5p mRNA expression in 29 colorectal cancer tissues grouped by rs2735383 C/G genotypes. The hsa-miR-509-5p mRNA expression was calculated relative to expression of <i>U6</i> mRNA. Data are mean ± standard error of the mean, <i>P</i> = 0.345.</p