Additional file 4: of Comprehensive circular RNA profiling reveals that circular RNA100783 is involved in chronic CD28-associated CD8(+)T cell ageing

Illustration of step-wised optimization on overlapping circRNAs between C1 and C4. (PPTX 66 kb

the radiation source signals for testing

In the paper, the radiation source signals for testing are from Case Western Reserve University Bearing Data Center. The database has been a standard dataset for testing the effectiveness of feature extraction algorithm and pattern recognition algorithm. Besides, the sampled signals of the database are full of random mechanical noise, which makes the test closer to the real situation. The motor drive end rotor is supported by a test bearing, where a single point of failure is set through discharge machining. The radiation source signals of bearing vibration data used for analysis are obtained under the motor speed of 1797 r/min and load of 0 horsepower. An accelerometer is installed on the motor drive end housing with a bandwidth of up to 5000 Hz, and the vibration data for the test bearing under different fault patterns is collected by a recorder as the radiation source signals, in which the sampling frequency is 12 kHz. The fault types contain outer race fault, the inner race fault, and the ball fault, and the fault diameters, i.e., fault severities, contain 28 mils, 21 mils, 14 mils and 7 mils. Totally 11 types of radiation source signals of bearing vibration data considering different fault categories and fault severities are analyzed, as seen in Table 1. Each data sample is made up of 2048 time series points. For those 550 data samples, each of those 550 data sample are different with different random mechanical noise. Among them, 110 data samples are chosen randomly for the establishment of the knowledge base, with the rest 440 data samples taken as testing data samples

Additional file 3: Figure S1. of Comprehensive circular RNA profiling reveals that circular RNA100783 is involved in chronic CD28-associated CD8(+)T cell ageing

Top-5 circRNA-miRNA networks using miRNAs with the 5 highest degrees. Figure S2. Distributions of miRNAs of Top10% Degrees in the Top-5 networks. Figure S3. Re-enriched networks using up-regulated miRNAs (left) and down-regulated miRNAs(right) of Top-10% highest Degree in the original Top-5 network. Figure S4. Distributions of Degree of the miRNAs in the re-enriched Top-10%-mi network. Figure S5. Distribution of Degree of the circRNAs in the re-enriched Top10%-ci network. Figure S6A. Venn’s diagram for optimized circRNA candidates between four groups. Figure S7. The screenshot of the online annotation for the targeted genes in the circRNA100783-targeted miRNA-gene network determined by DAVID annotation. (DOCX 1500 kb

Additional file 1: Table S1. of Comprehensive circular RNA profiling reveals that circular RNA100783 is involved in chronic CD28-associated CD8(+)T cell ageing

The top 10 differentiated circRNAs ranking by Fold Change (FC) in four comparison groups. Table S2. The RNA pooling and grouping protocol of the eight microarrays. Table S3-1. Distribution of the Top 10% Degree of up-regulated miRNAs (Degree range=22-60, n=75. Table S3-2. Distribution of the Top 10% Degree of down-regulated miRNAs (Degree range=28-81, n=80. Table S4. Five detailed annotation for the circRNA/miRNA interaction (circ100783 and its Top-5 predicted miRNA targets). Table S5. The detailed characteristicsof qRT-PCR for eight candidate circRNAs. (DOCX 344 kb

sj-eps-3-aph-10.1177_10105395241226525 – Supplemental material for Risk Factors of Premature Atherosclerotic Cardiovascular Disease in China: A Longitudinal Analysis of the China Health and Nutrition Survey Cohort

Supplemental material, sj-eps-3-aph-10.1177_10105395241226525 for Risk Factors of Premature Atherosclerotic Cardiovascular Disease in China: A Longitudinal Analysis of the China Health and Nutrition Survey Cohort by Yihong Ding, Yifan Zhou, Hui Han, Chen Chen and Yelena Tarasenko in Asia Pacific Journal of Public Health</p

sj-docx-1-aph-10.1177_10105395241226525 – Supplemental material for Risk Factors of Premature Atherosclerotic Cardiovascular Disease in China: A Longitudinal Analysis of the China Health and Nutrition Survey Cohort

Supplemental material, sj-docx-1-aph-10.1177_10105395241226525 for Risk Factors of Premature Atherosclerotic Cardiovascular Disease in China: A Longitudinal Analysis of the China Health and Nutrition Survey Cohort by Yihong Ding, Yifan Zhou, Hui Han, Chen Chen and Yelena Tarasenko in Asia Pacific Journal of Public Health</p

sj-eps-2-aph-10.1177_10105395241226525 – Supplemental material for Risk Factors of Premature Atherosclerotic Cardiovascular Disease in China: A Longitudinal Analysis of the China Health and Nutrition Survey Cohort

Supplemental material, sj-eps-2-aph-10.1177_10105395241226525 for Risk Factors of Premature Atherosclerotic Cardiovascular Disease in China: A Longitudinal Analysis of the China Health and Nutrition Survey Cohort by Yihong Ding, Yifan Zhou, Hui Han, Chen Chen and Yelena Tarasenko in Asia Pacific Journal of Public Health</p

Selectivity of Br/Li Exchange and Deprotonation of 4,4′-Dibromo-3,3′-bithiophene for Synthesis of Symmetrical and Unsymmetrical Dithienoheteroaromatic Rings

The novel selective synthesis of symmetrical and unsymmetrical dithienoheteroaromatic rings (<b>DTHA</b>s) has been developed via intramolecular cyclization of 4,4′-dibromo-3,3′-bithiophene (<b>3</b>). Four reaction conditions including <i>n</i>-BuLi/Et₂O, <i>n</i>-BuLi/THF, <i>s</i>-BuLi/Et₂O, and <i>t</i>-BuLi/Et₂O were employed to react with <b>3</b> for selective formation of two types of dicarbanions, which generate the symmetrical and unsymmetrical <b>DTHA</b>s after quenching with three electrophilic reagents (<b>4a</b>–<b>c</b>). The possible mechanism of formation of <b>DTHA</b>s was proposed. In addition, two unsymmetrical <b>DTHA</b>s were confirmed by X-ray single-crystal analyses

Selectivity of Br/Li Exchange and Deprotonation of 4,4′-Dibromo-3,3′-bithiophene for Synthesis of Symmetrical and Unsymmetrical Dithienoheteroaromatic Rings

The novel selective synthesis of symmetrical and unsymmetrical dithienoheteroaromatic rings (<b>DTHA</b>s) has been developed via intramolecular cyclization of 4,4′-dibromo-3,3′-bithiophene (<b>3</b>). Four reaction conditions including <i>n</i>-BuLi/Et₂O, <i>n</i>-BuLi/THF, <i>s</i>-BuLi/Et₂O, and <i>t</i>-BuLi/Et₂O were employed to react with <b>3</b> for selective formation of two types of dicarbanions, which generate the symmetrical and unsymmetrical <b>DTHA</b>s after quenching with three electrophilic reagents (<b>4a</b>–<b>c</b>). The possible mechanism of formation of <b>DTHA</b>s was proposed. In addition, two unsymmetrical <b>DTHA</b>s were confirmed by X-ray single-crystal analyses

Immobilization of Lipase from <i>Pseudomonas fluorescens</i> on Porous Polyurea and Its Application in Kinetic Resolution of Racemic 1‑Phenylethanol

A porous polyurea (PPU) was prepared through a simple protocol by reacting toluene diisocyanate with water in binary solvent of water–acetone. Its amine group was determined through spectrophotometric absorbance based on its iminization with <i>p</i>-nitrobenzaldehyde amines. PPU was then used as a novel polymer support for enzyme immobilization, through activation by glutaraldehyde followed by immobilization of an enzyme, lipase from <i>Pseudomonas fluorescens</i> (PFL), via covalent bonding with the amine groups of lipase molecules. Influences of glutaraldehyde and enzyme concentration and pH in the process were studied. The results revealed that the activity of the immobilized PFL reached a maximum at GA concentration of 0.17 mol/L and at pH 8. Immobilization rate of 60% or higher for PFL was obtained under optimized condition with an enzyme activity of 283 U/mg. The porous structure of PPU, prior to and after GA activation and PFL immobilization, was characterized. The activity of the immobilized PFL at different temperature and pH and its stability at 40 °C as well as its reusability were tested. The immobilized enzyme was finally used as enantioselective catalyst in kinetic resolution of racemic 1-phenylethanol (1-PEOH), and its performance compared with the free PFL. The results demonstrate that the enzyme activity and stability were greatly improved for the immobilized PFL, and highly pure enantiomers from racemic 1-PEOH were effectively achieved using the immobilized PFL. Noticeable deactivation of PFL in the resolution was observed by acetaldehyde in situ formed. In addition, the immobilized PFL was readily recovered from the reaction system for reuse. A total of 73% of the initial activity was retained after 5 repeated reuse cycles. This work provides a novel route to preparation of a polyurea porous material and its enzyme immobilization, leading to a novel type of immobilized enzyme for efficient kinetic resolution of racemic molecules