Antibiotic angucycline derivatives from the deepsea-derived <i>Streptomyces lusitanus</i>

A new (1, grincamycin L) and two known (2 and 3) angucycline derivatives were obtained from the fermentation of deepsea-derived Streptomyces lusitanus OUCT16-27 strain. The structures of 1–3 were elucidated based on the LC-MS analysis together with 1D and 2D NMR data assignment. In the antibacterial assay, 1 and 2 exhibited moderate growth inhibitions against multi-drug resistant (MDR) strains of E. faecium, E. faecalis and S. aureus with the minimum inhibitory concentrations (MICs) of 3.12–6.25 µg/mL.</p

Antibiotic Dixiamycins from a Cold-Seep-Derived <i>Streptomyces olivaceus</i>

Two new (1 and 2) along with six known (3–8) dixiamycins were isolated from the culture broth of a cold-seep-derived actinomycete, Streptomyces olivaceus OUCLQ19-3. Structures of the isolated compounds were elucidated based on extensive MS and NMR spectroscopic analyses together with ECD calculations. In the antibacterial test, compounds 1–8 exhibited notable growth inhibitions against a panel of multi-drug-resistant (MDR) strains with MIC values of 0.78–6.25 μg/mL, among which 1, 2, and 5–7 were more potent than the positive control tetracycline

Amphonal, a new polyene aldehyde from a deep-sea-derived <i>Streptomyces amphotericinicus</i>

A new polyene aldehyde, named amphonal (1), and two known (2 and 3) polyketides were isolated from the deep-sea-derived Streptomyces amphotericinicus OUCT16-38 strain. The structure of 1 was determined by extensive MS and NMR spectroscopic analysis. In the cytotoxicity evaluation, compound 2 showed significant growth inhibition against the drug-resistant human lung cancer cell line A549-Taxol with IC50 value of 0.44 μM, which was more potent than the positive control doxorubicin. Meanwhile, 2 showed considerable cytotoxic effect towards H1975, H1299 and HEL cell lines (IC50 = 0.93-4.73 μM) as well.</p

Genome-Guided Discovery of Antifungal Filipins from a Deep-Sea-Derived <i>Streptomyces antibioticus</i>

Nine new (1–3, 5–8, 11, and 12; named filipins VI–XIV) and three known (4, 9, and 10) filipin-type polyene macrolides were isolated from the deep-sea-derived Streptomyces antibioticus OUCT16-23 using a genome-guided strategy coupled with bioassay. Their structures were elucidated based on the extensive MS and NMR spectroscopic analyses together with ECD calculations. In an antifungal assay, compounds 4, 5, and 7–10 showed different degrees of growth inhibition against Candida albicans with minimum inhibitory concentrations (MICs) of 1.56–12.5 μg/mL, by which the alkyl side-chain substitution affecting the activity was preliminarily studied. A biosynthetic pathway to 1–12 in S. antibioticus OUCT16-23 is also proposed

MOESM1 of Overexpression of a type III PKS gene affording novel violapyrones with enhanced anti-influenza A virus activity

Additional file 1: Table S1. Plasmids and strains used in this study. Table S2. Primer pairs used in this study. Table S3. Homologous locus of vioAB in different Streptomyces genomes. Figure S1. Relative yields for compounds 1–14 in different strains. Figure S2. Spectral data of 1. Figure S3. Spectral data of 2. Figure S4. Spectral data of 3. Figure S5. Spectral data of 4. Figure S6. Spectral data of 5. Figure S7. Spectral data of 6. Figure S8. Spectral data of 7. Figure S9. Spectral data of 8. Figure S10. Spectral data of 9. Figure S11. Spectral data of 10. Figure S12. Spectral data of 11. Figure S13. Spectral data of 12. Figure S14. Spectral data of 13. Figure S15. Spectral data of 14. Figure S16. Multiple-sequence alignments of VioA with selected type III PKSs. Figure S17. Site-directed mutagenesis study of VioA

MOESM1 of Activation of a plasmid-situated type III PKS gene cluster by deletion of a wbl gene in deepsea-derived Streptomyces somaliensis SCSIO ZH66

Additional file 1: Table S1. Anti-MRSA activities of violapyrones (VLPs 1–5). Table S2. Bacteria and plasmids used in this study. Table S3. The primer pairs used for cosmid library screening. Table S4. The primer pairs used for PCR-targeted mutagenesis. Table S5. The primer pairs used for PCR confirmation of the mutants. Table S6. The primer pairs used for qPCR analysis. Figure S1. Inactivation of wblA so . Figure S2. Spectral data of VLP B, 1. Figure S3. Inactivation of pksIII-1. Figure S4. Inactivation of vioA. Figure S5. Inactivation of vioB. Figure S6. Inactivation of orf1. Figure S7. Inactivation of orf(-1-2). Figure S8. Spectral data of VLP J, 3. Figure S9. Spectral data of VLP A, 2. Figure S10. Spectral data of VLP C, 4. Figure S11. Spectral data of VLP H, 5. Figure S12. Phenotypes of the S. somaliensis SCSIO ZH66 strains

The number of students evacuated through routes 1–3, the front and back door during the four evacuation stages in the No. 2 Middle School of Ganqika.

The number of students evacuated through routes 1–3, the front and back door during the four evacuation stages in the No. 2 Middle School of Ganqika.</p

Social context.

Notes: WP-With patients; WFA-With family; WS-With strangers; WFR-With friends; Wcoll-With colleagues; Wclas-With classmates.</p

Average evacuation speeds for different seismic intensities and locations.

<p>Average evacuation speeds for different seismic intensities and locations.</p