Geometric Pseudospectral Method on SE(3) for Rigid-Body Dynamics with Application to Aircraft

General pseudospectral method is extended to the special Euclidean group SE(3) by virtue of equivariant map for rigid-body dynamics of the aircraft. On SE(3), a complete left invariant rigid-body dynamics model of the aircraft in body-fixed frame is established, including configuration model and velocity model. For the left invariance of the configuration model, equivalent Lie algebra equation corresponding to the configuration equation is derived based on the left-trivialized tangent of local coordinate map, and the top eight orders truncated Magnus series expansion with its coefficients of the solution of the equivalent Lie algebra equation are given. A numerical method called geometric pseudospectral method is developed, which, respectively, computes configurations and velocities at the collocation points and the endpoint based on two different collocation strategies. Through numerical tests on a free-floating rigid-body dynamics compared with several same order classical methods in Euclidean space and Lie group, it is found that the proposed method has higher accuracy, satisfying computational efficiency, stable Lie group structural conservativeness. Finally, how to apply the previous discretization scheme to rigid-body dynamics simulation and control of the aircraft is illustrated

Olf1/EBF associated zinc finger protein interfered with antinuclear antibody production in patients with systemic lupus erythematosus

Abstract Introduction The aim of the study was to determine whether Olf1/EBF associated zinc finger protein (OAZ), a transcription factor encoded by a positional systemic lupus erythematosus (SLE) candidate gene, plays a functional role in the pathogenesis in SLE. Methods Gene expression levels in peripheral blood cells (PBLs) measured using quantitative real-time polymerase chain reaction (qPCR) were assessed for association with disease activity and the presence of specific autoantibodies. Peripheral blood mononuclear cells (PBMCs) were incubated with specific siRNAs for three days, then cells were harvested for measuring mRNA levels using qPCR, and supernatants for levels of total immunoglobulin (Ig)G and IgM as well as secreted cytokines, chemokine and antinuclear antibodies (ANA) using ELISA. Indirect immunofluorescence was also applied for ANA detection. Results OAZ gene expressions in PBLs from 40 ANA-positive SLE patients were significantly increased than those from 30 normal controls (P < 0.0001) and 18 patients with rheumatoid arthritis (P < 0.01). In SLE patients, OAZ transcripts were positively correlated with SLE disease activity index (SLEDAI) score (r = 0.72, P < 0.0001) and higher in those positive for anti-dsDNA or anti-Sm antibodies (both P < 0.05). Co-culturing with OAZ siRNAs reduced mRNA levels of OAZ by 74.6 ± 6.4% as compared to those co-cultured with non-targeting siRNA and OAZ silencing resulted in reduced total IgG, ANA, interferon (IFN)-γ, interleukin (IL)-10, IL-12 and IL-21, but elevated CCL2 levels in culture supernatants (P < 0.05). The declined ANA levels correlated with inhibited OAZ expression (r = 0.88, P = 0.05), reduced IL-21 levels (r = 0.99, P < 0.01), and elevated chemokine (C-C motif) ligand 2 levels (r = -0.98, P < 0.01). Expressions of ID1-3 were significantly down-regulated by 68.7%, 70.2% and 67.7% respectively after OAZ silence, while ID3 was also highly expressed in SLE PBLs (P < 0.0001) and associated with disease activity (r = 0.76, P < 0.0001) as well as anti-dsDNA or anti-Sm antibodies (both P < 0.05). Conclusions Elevated expression of OAZ transcripts in SLE PBLs were strongly correlated with disease activity. Suppression of OAZ expression inhibited downstream ID levels, and secretion of ANA and IL-21, implicating a role of OAZ pathway in the pathogenesis of SLE

Consensusabilization for Continuous-Time High-Order Multiagent Systems with Time-Varying Delays

For the consensus problems of high-order linear multiagent systems with time-varying delays in directed topologies, the LMI based-consensus criterion and NLMI-based consensusabilization (protocol parameters design that makes the multiagent systems achieve consensus) are investigated. Improved Lyapunov-Krasovskii functional is used for establishing the consensus convergence criteria and deriving the corresponding consensus protocol. In order to reduce the conservativeness, some proper free-weighting matrices are added into the derivative of Lyapunov-Krasovskii functional and that only keeps one necessary zoom. The numerical and simulation examples are given to demonstrate the effectiveness of the theoretical results. Compared with existing literatures, the convergence criterion and protocol design proposed have lower conservativeness

Monoclonal Antibodies against Accumulation-Associated Protein Affect EPS Biosynthesis and Enhance Bacterial Accumulation of Staphylococcus epidermidis

Because there is no effective antibiotic to eradicate Staphylococcus epidermidis biofilm infections that lead to the failure of medical device implantations, the development of anti-biofilm vaccines is necessary. Biofilm formation by S. epidermidis requires accumulation-associated protein (Aap) that contains sequence repeats known as G5 domains, which are responsible for the Zn2+-dependent dimerization of Aap to mediate intercellular adhesion. Antibodies against Aap have been reported to inhibit biofilm accumulation. In the present study, three monoclonal antibodies (MAbs) against the Aap C-terminal single B-repeat construct followed by the 79-aa half repeat (AapBrpt1.5) were generated. MAb18B6 inhibited biofilm formation by S. epidermidis RP62A to 60% of the maximum, while MAb25C11 and MAb20B9 enhanced biofilm accumulation. All three MAbs aggregated the planktonic bacteria to form visible cell clusters. Epitope mapping revealed that the epitope of MAb18B6, which recognizes an identical area within AapBrpt constructs from S. epidermidis RP62A, was not shared by MAb25C11 and MAb20B9. Furthermore, all three MAbs were found to affect both Aap expression and extracellular polymeric substance (EPS, including extracellular DNA and PIA) biosynthesis in S. epidermidis and enhance the cell accumulation. These findings contribute to a better understanding of staphylococcal biofilm formation and will help to develop epitope-peptide vaccines against staphylococcal infections

The oyster genome reveals stress adaptation and complexity of shell formation

The Pacific oyster Crassostrea gigas belongs to one of the most species-rich but genomically poorly explored phyla, the Mollusca. Here we report the sequencing and assembly of the oyster genome using short reads and a fosmid-pooling strategy, along with transcriptomes of development and stress response and the proteome of the shell. The oyster genome is highly polymorphic and rich in repetitive sequences, with some transposable elements still actively shaping variation. Transcriptome studies reveal an extensive set of genes responding to environmental stress. The expansion of genes coding for heat shock protein 70 and inhibitors of apoptosis is probably central to the oyster's adaptation to sessile life in the highly stressful intertidal zone. Our analyses also show that shell formation in molluscs is more complex than currently understood and involves extensive participation of cells and their exosomes. The oyster genome sequence fills a void in our understanding of the Lophotrochozoa. © 2012 Macmillan Publishers Limited. All rights reserved

Am-driven design of hydraulic manifolds: enhancing fluid flow and reducing weight

Selective laser melting (SLM), one type of metal additive manufacturing (AM) technology, uses a highintensity laser to selectively melt pre-spread metal powders by a layer-on-layer manner. The technology does not only provide a new way of manufacturing but also innovates product design methodology. In this study, a hydraulic block manifold is designed and manufactured using SLM. In this paper, we present an AM-driven design approach of hydraulic manifolds based on a case study. The target is not only to reduce weight but also to enhance fluid flow by optimizing fluid path to reduce pressure drop. The novelty of the research includes developing a design approach of hydraulic manifolds using SLM with a particular focus on fluid flow. Compared to the traditional hydraulic manifold, the weight of the new SLMed hydraulic manifold was reduced by more than 80%, size by half. Pressure loss of the main functional oil circuit was reduced by 31%, illustrating that the new hydraulic manifold design simultaneously achieves lightweight and high performance. This study contributes to providing theoretical guidance to the design of additively manufactured hydraulic components with high performance

Interface behaviors and mechanical properties of diffusion bonded 45 steel/additive manufactured 316L steel joints

Diffusion bonding of additive manufactured (AM) metal parts to traditionally manufactured metal parts could simultaneously achieve large-scale, complex-structure, and multi-material parts. However, there is a lack of study on diffusion bonding as well as its interface formation mechanism between AM metal parts and traditionally manufactured metal parts. In this study, diffusion bonding was performed to join AM 316L stainless steel and casting 45 steel at different temperatures. The element distribution, interface characteristics and shear strength of the joints were investigated, and the interface formation mechanism at different bonding temperature was analyzed in detail. The results reveal that the interface transition zone from the AM 316L steel side to the 45 steel side is composed of the metallic elements diffusion layer and the carbide layer. Moreover, an increase in bonding temperature can effectively promote interface voids closure and increase the thickness of the metallic elements diffusion layer, while the thickness of the carbide layer firstly increases and then decreases as the temperature increases. The component of the carbide layer was identified as brittle compounds (CrFe)23C6 and (CrFe)7C3. The shear strength of the joints exhibited an increasing trend with the increasing bonding temperature. Interestingly, a sudden decrease in strength was found at 950 °C due to the significant increase of the brittle carbide layer, while high-quality joints could be achieved as the brittle carbides dissolve at 1000 °C. This study could lay the foundation for understanding diffusion bonding interface behaviors between AM metal parts and traditional casting metal parts

Colourful 3D anti-counterfeiting label using nanoscale additive manufacturing

Microprinting is changing anti-counterfeit technology by producing complex micro-/nano-scale labels to increase the security level. However, the current microprinted anti-counterfeiting labels only contain 2D features, thought of as single coding (e.g. mostly in texts or graphs), unable to satisfy the high-end anti-counterfeiting demands of producing multi-coding, high-storage-density labels. Here, we introduce an approach for fluorescence printing to produce a 3D colourful, sophisticated anti-counterfeiting label. In the method, pre-synthetic QDs are printed with photoresists based on two-photon polymerisation, allowing for accurate regulation of fluorescence in both 2D and 3D. The fabricated photoresists exhibit immediate and postprocessing-free colour emissions under UV light. We demonstrated the ultra-high free design and accurate manufacturing capability of the approach by fabricating multi-colour, multi-layer patterns containing both texts and graphs on a 50μm × 50μm × 30μm portable label. The approach can also be applied to multi-dimensional optical data storage and colourful microdisplays

Surface nanocrystallization mechanism of additive manufactured 316L stainless steel via high-energy shot peening

High-energy shot peening (HESP) is an effective way to achieve surface nanocrystallization modification of additive manufactured metal parts, significantly improving their mechanical properties. Yet, there is still a lack of deep understanding of the surface nanocrystallization evolution mechanism of additive manufactured metals. For that, this study focuses on the surface nanocrystallization mechanism of selective laser melted 316L stainless steel treated by HESP treatment. The detailed microstructural characteristics are investigated via optical microscope (OM) and transmission electron microscopy (TEM). The experimental results show that the matrix layer, the low-strain matrix layer, the elongated ultrafine grain layer, the short rod-like ultrafine grain layer, and the equiaxed nanograin layer occurred in sequence from the matrix to the topmost surface. HESP treatment enables the gradient grain refinement from the matrix with a micrometer structure of about 120 mm to the topmost surface with an average grain size of about 25 nm. The evolution of surface nanocrystallization has resulted from the interaction of high-density dislocation, dislocation tangles (DTS), and twins. This study could provide a deep understanding of the surface nanocrystallization evolution mechanism of additive manufactured metal parts treated by HESP

Assessment of friction loss to horizontally built fluid passages using additive manufacturing

Selective laser melting (SLM), is a type of additive manufacturing, which selectively melts a pre-spread layer of metal powders and produce a part by a layer-on-layer manner. SLM has demonstrated a great potential to reduce size and weight in hydraulic manifolds. However, a theoretical base is lacking since friction loss is unclear in a SLMed fluid passage. In this study, various fluid passages without supports, from diameters from 4 mm to 16 mm, were produced horizontally using SLM. The profile was measured using a 3D scanner and surface roughness was measured using a confocal laser scanning microscope. Friction factor was studied using simulation, experiments, and classical theory. The hydraulic diameter of the SLMed passages is smaller than the design diameter. Surface roughness is extremely high on the top part of the inner wall while the rest part is around 10 μm. Such trends are irrelevant of passage diameters. Friction factors in SLMed passage is much larger than those predicted using Moody theory, particularly in laminar flow. The transition from laminar flow to turbulent flow appears at a smaller Reynolds number with increased passage diameter. The influence of the profile overweighs that of the surface roughness on friction factor