Additional file 2 of Downregulation of CDK5 signaling in the dorsal striatum alters striatal microcircuits implicating the association of pathologies with circadian behavior in mice

Additional file 2: Fig. S1. (A) Cell morphology in the DS area was detected by HE staining. The striatal neuron in DS-CDK5-KD mice were morphologically normal and showed no significant loss. Scale bars = 20 μm. (B) Left panel: detrimental effects in the DS area of CDK5-KD were detected by Nissl staining. Right panel: quantitative profiles of Nissl bodies in the DS area of WT, DS-CDK5-NC, and DS-CDK5-KD mice. Nissl staining revealed no detrimental effects induced by the delivery of LV/Cas9-CDK5-sgRNA into the DS (Nissl body, WT: 7.467 ± 0.55, DS-CDK5-KD: 7.733 ± 0.51, DS-CDK5-KD: 6.733 ± 0.45). Arrows: Nissl body. Scale bars = 0 μm. (C) Left panel: cell apoptosis in the DS area was detected by TUNEL staining. Right panel: quantitative profiles of TUNEL-positive cells in the DS area of WT, DS-CDK5-NC and DS-CDK5-KD mice. Quantitative analysis for the TUNEL-positive cells showed there were no significant difference among in WT, DS-CDK5-NC, and DS-CDK5-KD mice (TUNEL-positive cell, WT: 2.47± 0.27, DS-CDK5-KD: 3.07 ± 0.27, DS-CDK5-KD: 3.33 ± 0.32). Arrows: TUNEL-positive cells. Scale bars = 20 μm. The TUNEL-positive cells showed the typical morphological features of apoptosis such as chromatin condensation, cytoplasmic budding and apoptotic bodies. Data are represented as the mean ± SEM, n = 15. One-way ANOVA, Tukey’s multiple comparisons test

Additional file 1 of Downregulation of CDK5 signaling in the dorsal striatum alters striatal microcircuits implicating the association of pathologies with circadian behavior in mice

Additional file 1. Additional methods