GO categories of biological process (BP), cellular component (CC) and molecular function (MF) for the early water-stress responding genes (P-6h <i>vs</i>. P-0h) in <i>A</i>. <i>sparsifolia</i> primary roots.

<p>(A) the early water-stress inducible genes; (B) the early water-stress repressed genes. The right y-axis shows the number of genes in a category, and the left y-axis indicates the percentage of a specific category of genes in that main category.</p

Summary of unigene annotations against public databases.

<p>Abbreviations: NR, non-redundant protein sequence; NT, non-redundant nucleotide; Pfam, Protein Family Database; KEGG, Kyoto Encyclopedia of Genes and Genomes database; KOG, euKaryotic Ortholog Groups; GO, Gene Ontology.</p><p>Summary of unigene annotations against public databases.</p

Effect of water stress induced by PEG-6000 on development of the <i>Alhagi sparsifolia</i> primary roots.

<p>(A) Seeds of <i>A</i>. <i>sparsifolia</i> (Scale bar = 2.0 cm). (B) Typical phenotype of the <i>A</i>. <i>sparsifolia</i> seedlings at 7 days post treatment with PEG-6000 (Scale bar = 1.0 cm). The <i>A</i>. <i>sparsifolia</i> seeds were first treated with concentrated sulfuric acid (98%) for 20 min, and then put on fully wetted filter paper at 25°C in the dark for 24 h. The germinated seeds were selected and transferred to petri dishes containing filter paper saturated with the different percentages of PEG-6000 solution (0, 90, 150, 220, 270 and 320 g/L), and were remained at 25°C in the dark for 3 days followed by 4 additional days but under 16-h light/8-h dark. At least 15 seedlings were included in each different treatment, and three independent experiments were preformed. (C) Average length of the primary roots of <i>A</i>. <i>sparsifolia</i> treated with PEG-6000 at 7 days. Error bars represent SE. P values determined by Student t test (** p < 0.01).</p

Venn diagrams showing the number of the common and specific DEGs in P-6h, P-24h and Rh-6h.

<p>The analysis were based either on genes up regulated in P-6h and P-24h (compared to P-0h) and down regulated in Rh-6h (compared to P-24h) (A), or on genes down regulated in P-6h and P-24h (compared to P-0h) and up regulated in Rh-6h (compared to P-24h) (B).</p

KOG function classification of the transcriptome of the <i>A</i>. <i>sparsifolia</i> primary roots.

<p>Out of 24,639 NR hits and 16,417 PFAM homologies, 9,313 sequences have a KOG classification among the 26 categories.</p

Overview of the sequencing reads.

<p>a, L1: Reads sequencing from the left; R2: Reads sequencing from the right.</p><p>b, adaptors and low-quality reads were excluded.</p><p>c, Q20: The percentage of bases with quality value larger than 20.</p><p>d, Q30: The percentage of bases with quality value larger than 30.</p><p>Overview of the sequencing reads.</p

GO categories of biological process, cellular component and molecular function for the transcriptome of the <i>A</i>. <i>sparsifolia</i> primary roots.

<p>The right y-axis shows the number of genes in a category, and the left y-axis indicates the percentage of a specific category of genes in that main category.</p

Characteristics of homology search of unigenes against the NR database.

<p>(A) E-value distribution of the top BLAST hits for each unigene with a cut-off E-value of 1.0E-5. (B) Similarity distribution of the best BLAST hits for each unigene. (C) Species distribution is shown as the percentage of the total homologous sequences with an E-value of at least 1.0E-5. We used all plant proteins in the NCBI NR database for homology search and extracted the first hit of each sequence for analysis.</p

Statistics of clean reads mapped to the primary root transcriptome of <i>A</i>. <i>sparsifolia</i>.

<p>a. The conservative degree of mismatch was no more than 2 bp.</p><p>Statistics of clean reads mapped to the primary root transcriptome of <i>A</i>. <i>sparsifolia</i>.</p

GO categories of biological process (BP), cellular component (CC) and molecular function (MF) for the late water-stress responding genes (P-24h <i>vs</i>. P-0h) in <i>A</i>. <i>sparsifolia</i> primary roots.

<p>(A) the late water-stress inducible genes; (B) the late water-stress repressed genes. The right y-axis shows the number of genes in a category, and the left y-axis indicates the percentage of a specific category of genes in that main category.</p