Marginal effects of explanatory variables in REMLM.

Marginal effects of explanatory variables in REMLM.</p

Characteristics of four selected signalized intersections.

Characteristics of four selected signalized intersections.</p

Ratios of road and traffic management conditions at each signalized intersection.

Ratios of road and traffic management conditions at each signalized intersection.</p

Time distribution of traffic violations.

Time distribution of traffic violations.</p

Descriptions of independent variables.

Descriptions of independent variables.</p

Layouts of the four selected signalized intersections.

Layouts of the four selected signalized intersections.</p

Depletion of mmu_circ_0001751 (circular RNA Carm1) protects against acute cerebral infarction injuries by binding with microRNA-3098-3p to regulate acyl-CoA synthetase long-chain family member 4

Circular RNAs (circRNAs) play a critical role in acute cerebral infarction (ACI). Our research discussed the effect of circ-Carm1 in ACI and its potential molecular mechanisms. Healthy controls and patients with ACI were included in this study. The establishment of an oxygen and glucose deprivation/reoxygenation (OGD/R) model of HT22 cells was conducted to mimic ACI in vitro. Quantitative reverse transcription polymerase chain reaction was conducted to determine mRNA levels extracted from serum and HT22 cell samples, and Western blotting was performed to determine protein levels. Terminal deoxynucleotidyl transferase dUTP nick end labeling and cell counting kit 8 assays were conducted to evaluate cellular functions. Concentrations of Fe2+ and malondialdehyde, and levels of transferrin receptor 1, glutathione peroxidase 4, and glutathione were evaluated to determine ferroptosis in OGD/R-induced HT22 cells. The binding relationships between mRNAs and miRNAs were verified. circ-Carm1 was highly expressed in OGD/R-treated HT22 cells. Deficiency of circ-Carm1 restored cell viability and suppressed ferroptosis in OGD/R-induced HT22 cells. miR-3098-3p was predicted to be a target of circ-Carm1. The miR-3098-3p inhibitor partly neutralized the functions of circ-Carm1 in OGD/R-induced HT22 cells. Furthermore, acyl-CoA synthetase long-chain family member 4 (ACSL4) was confirmed to be a downstream target of miR-3098-3p and was elevated in OGD/R-induced HT22 cells. Overexpression of ACSL4 mitigated the functions of miR-3098-3p and accelerated HT22 cell dysfunction. Hence, circ-Carm1 is upregulated in ACI. circ-Carm1 suppression protects HT22 cells from dysfunction by inhibiting ferroptosis. Therefore, inducing circ-Carm1 deficiency may be a promising therapeutic method for ACI.</p

Number of traffic violations under various weather conditions.

Number of traffic violations under various weather conditions.</p

Estimation results of OMLM and REMLM of VTM.

Estimation results of OMLM and REMLM of VTM.</p

NMR Studies of Structure and Reorganization Dynamics of an [⁶Li]-Allylic Lithium Compound Complexed to [¹⁴N,¹⁵N]-<i>N</i>,<i>N</i>,<i>N</i>‘,<i>N</i>‘-Tetramethylethylenediamine: Inversion and Ligand Lithium Exchange

Proton, 13C, 6Li, and 15N NMR line-shape studies of exo,exo-1-trimethylsilyl-3-(dimethylethylsilyl)allyllithium-6Li complexed to [14N,15N]-N,N,N‘,N‘-tetramethylethylenediamine (TMEDA) 2 as a function of temperature and of added diamine reveal the dynamics of three fast equilibrium reorganization processes. These are (with ΔH⧧ values in kilocalories per mole and ΔS⧧ values in entropic units):  mutual exchange of lithium between two 2 molecules (6.3, −21), exchange of TMEDA between its free and complexed states (5.0 and −22), and first-order transfer of complexed ligand between the allyl faces (7.0 and −20). Intermediates that are dimeric in TMEDA are proposed for the first two of these reorganization processes