Separation and Identification of Anthocyanins Extracted from Blueberry Wine Lees and Pigment Binding Properties toward β‑Glucosidase

Anthocyanins were isolated from blueberry wine lees using Sephadex LH-20 column chromatography and semipreparative high-performance liquid chromatography (semipreparative HPLC) and then identified by HPLC-DAD-ESI-MS/MS. Our results show that malvidin-3-hexose (Mv-3-hex) and malvidin-3-(6′acetyl)-hexose (Mv-3-ace-hex) are the major components in the anthocyanin extracts of blueberry wine lees (>90%). The binding characteristics of Mv-3-hex and Mv-3-ace-hex with β-glucosidase were investigated by fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and molecular docking. Spectroscopic analysis revealed that β-glucosidase fluorescence quenched by Mv-3-hex and Mv-3-ace-hex follows a static mode. Binding of Mv-3-hex and Mv-3-ace-hex to β-glucosidase mainly depends on electrostatic force. The result from CD spectra shows that adaptive structure rearrangement and increase of β-sheet structure occur only in the presence of Mv-3-ace-hex. A molecular docking study suggests that Mv-3-ace-hex has stronger binding with β-glucosidase than Mv-3-hex

Bioinspired Interface Engineering for Moisture Resistance in Nacre-Mimetic Cellulose Nanofibrils/Clay Nanocomposites

The interfacial adhesion design between “mortar” and “bricks” is essential for mechanical and barrier performance of nanocellulose-based nacre-mimetic nanocomposites, especially at high moisture conditions. To address this fundamental challenge, dopamine (DA) has been conjugated to cellulose nanofibrils (CNFs) and subsequently assembled with montmorillonite (MTM) to generate layered nanocomposite films inspired by the strong adhesion of mussel adhesive proteins to inorganic surfaces under water. The selective formation of catechol/metal ion chelation and hydrogen bonding at the interface between MTM platelets and CNFs bearing DA renders transparent films with strong mechanical properties, particularly at high humidity and in wet state. Increasing the amount of conjugated DA on CNFs results in nanocomposites with increased tensile strength and modulus, up to 57.4 MPa and 1.1 GPa, respectively, after the films are swollen in water. The nanocomposites also show excellent gas barrier properties at high relative humidity (95%), complementing the multifunctional property profile

Cellulose-Based Composite Macrogels from Cellulose Fiber and Cellulose Nanofiber as Intestine Delivery Vehicles for Probiotics

Cellulose-based composite macrogels made by cellulose fiber/cellulose nanofiber (CCNM) were used as an intestine delivery vehicle for probiotics. Cellulose nanofiber (CNF) was prepared by a 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation system, and the carboxyl groups in CNF acted as pore size and pH responsibility regulators in CCNMs to regulate the probiotics loading and controlled release property. The macrogel presented a porosity of 92.68% with a CNF content of 90%, and the corresponding released viable <i>Lactobacillus plantarum</i> (<i>L. plantarum</i>) was up to 2.68 × 10⁸ cfu/mL. The porous structure and high porosity benefited <i>L. plantarum</i> cells to infiltrate into the core of macrogels. In addition, the macrogels made with high contents of CNF showed sustainable release of <i>L. plantarum</i> cells and delivered enough viable cells to the desired region of intestine tracts. The porous cellulose macrogels prepared by a green and environmental friendly method show potential in the application of fabricating targeted delivery vehicles of bioactive agents

data of article and supporting information

The files of SEM-PSP, IR and TG, data of performance, XPS and XRD are the data of articles. While the files of supporting information-IR and supporting information-data of recycle performance are the data of supporting informaton

The related XPS spectra of composites. from Novel amphiphilic polyvinylpyrrolidone functionalized silicone particles as carrier for low-cost lipase immobilization

Figure S1. XPS spectra of Si spectra of PVP-SP, PVP-SP@CRL. Table S1. The data of the each peak for the supporting materials. Figure S2. XPS spectra of C 1s spectra of PVP-SP, PVP-SP@CRL. Table S2. The data of the each peak for the supporting materials. Figure S3. XPS spectra of N 1s spectra of PVP-SP, PVP-SP@CRL. Table S3. The data of the each peak for the supporting materials. Fig S4. The FTIR spectra of SP/PVP, SP/Pst-b, SP/Pst-e, SP/Pst-b@CRL, SP/Pst-e@CRL and SP-PVP@CRL. Fig S5. Reusability of the three immobilization lipases