6 research outputs found
内臟に發生せる稀有なる多發性神經纎維腫症の一剖檢例
Information on the 74 candidate blast resistant genes in LAFBRs. (XLSX 90 kb
Comprehensive Profiling of the Rice Ubiquitome Reveals the Significance of Lysine Ubiquitination in Young Leaves
Protein
ubiquitination is a major post-translational modification
that regulates development, apoptosis, responses to environmental
cues, and other processes in eukaryotes. Although several ubiquitinated
proteins have been identified in rice, large-scale profiling of the
rice ubiquitome has not been reported because of limitations in the
current analytical methods. Here, we report the first rice ubiquitome,
determined by combining highly sensitive immune affinity purification
and high resolution LC–MS/MS. We identified 861 di-Gly-Lys-containing
peptides in 464 proteins in rice leaf cells. Bioinformatic analyses
of the ubiquitome identified a variety of cellular functions and diverse
subcellular localizations for the ubiquitinated proteins, and also
revealed seven putative ubiquitination motifs in rice. Proteins related
to binding and catalytic activity were predicted to be the preferential
targets of lysine ubiquitination. A protein interaction network and
KEGG analysis indicated that a wide range of signaling and metabolic
pathways are modulated by protein ubiquitination in rice. Our results
demonstrate the usefulness of the significantly improved method for
assaying proteome-wide ubiquitination in plants. The identification
of the 464 ubiquitinated proteins in rice leaves provides a foundation
for the analysis of the physiological roles of these ubiquitination-related
proteins
Additional file 1: Table S1. of A Genome-Wide Association Study of Field Resistance to Magnaporthe Oryzae in Rice
Rice blast evaluation of the RDP1 in three blast nurseries in the field. (XLSX 39 kb
Additional file 2: Table S2. of Genome-wide Association Mapping of Cold Tolerance Genes at the Seedling Stage in Rice
Cold tolerance distribution of the subpopulations in the rice diversity panel 1 (RDP1). (DOCX 44Â kb
Additional file 1: Table S1. of Genome-wide Association Mapping of Cold Tolerance Genes at the Seedling Stage in Rice
The cold tolerance scores of the evaluated rice accessions. (DOCX 40Â kb
Additional file 3: Table S3. of Genome-wide Association Mapping of Cold Tolerance Genes at the Seedling Stage in Rice
QTL lists for rice cold tolerance at the seedling stage in the RDP1 accessions. (PDF 214Â kb