症例の予後改善のための,電子ビームCT,4列～320列CTを用いた循環器領域の新しい臨床診断学の開発への貢献

I went to the Stanford University Department of Radiology\u27s three-dimensional (3D) imaging laboratory from 1996 to 1999 to study a novel 3D image processing technique using electron beam computed tomography (CT). When I returned to Japan, I found that multi-slice CT had been available in daily practice since 1998. We have published a total of 152 peer-reviewed papers on diagnostic images in the field of cardiovascular disease. In 2003, when 16-slice CT was available for use in general hospitals, we successfully developed a prototype 256-slice cone-beam CT at the National Institute of Radiological Sciences. We produced several papers discussing the utilities of this prototype CT in both animal and phantom experiments, the concepts and ideas that were currently used for cardiac perfusion and myocardium characteristic study. In 2010, our paper was used as a reference in the American College of Cardiology Foundation Expert Consensus Guideline. The our current topics presented include coronary artery stenosis, coronary arterial plaques, the characteristics of the myocardium, the anatomy of structural and congenital heart disease, and the cardiac function, all using 16-320 slice CT with reduced radiation exposure in CT acquisition. Furthermore, we are now performing novel clinical CT studies combined magnetic resonance imaging (MRI), positron emission tomography, and echocardiography. Using previous image data, we analyzed an epidemiology study using CT findings to predict the occurrence of major cardiovascular adverse events over long-term follow-up periods of more than 100 months (median), one of the longest follow-up periods documented in the literature. We also need to obtain accurate diagnoses for subjects with cardiac failure or fatal arrhythmia of unknown origin, allowing them to receive specific effective therapy for their possible cardiac amyloidosis, cardiac sarcoidosis, or Fabry\u27s disease. Of course, in all CT imaging techniques used for evaluation and monitoring of cardiovascular risk

Effect of Carboxylate Ligands on Alkane Dehydrogenation with (^<i>dm</i>Phebox)Ir Complexes

A series of carboxylate-ligated iridium complexes (^<i>dm</i>Phebox)­Ir­(O₂CR)₂(H₂O) (R = −CH₃, −CH₂CH₃, −CMe₃, −CH₂C₆H₅, and −CHCMe₂) were designed and synthesized to understand the carboxylate ligand effects on the reactivity of the complex for alkane dehydrogenation. Kinetic studies showed that the different R groups of the carboxylate iridium complexes can affect the reactivity with octane in the β-H elimination step. The rate constants for octene formation with different carboxylate ligands follow the order R = −CHCMe₂ > −CMe₃ > −CH₂CH₃ > −CH₃ > −CH₂C₆H₅. In contrast, there is no significant effect of carboxylate ligand on the rate of the C–H activation step at 160 °C. These experimental results support the findings in the previously reported density functional theory study of the (^<i>dm</i>Phebox)Ir complex in alkane C–H activation

Effect of Carboxylate Ligands on Alkane Dehydrogenation with (^<i>dm</i>Phebox)Ir Complexes

A series of carboxylate-ligated iridium complexes (^<i>dm</i>Phebox)­Ir­(O₂CR)₂(H₂O) (R = −CH₃, −CH₂CH₃, −CMe₃, −CH₂C₆H₅, and −CHCMe₂) were designed and synthesized to understand the carboxylate ligand effects on the reactivity of the complex for alkane dehydrogenation. Kinetic studies showed that the different R groups of the carboxylate iridium complexes can affect the reactivity with octane in the β-H elimination step. The rate constants for octene formation with different carboxylate ligands follow the order R = −CHCMe₂ > −CMe₃ > −CH₂CH₃ > −CH₃ > −CH₂C₆H₅. In contrast, there is no significant effect of carboxylate ligand on the rate of the C–H activation step at 160 °C. These experimental results support the findings in the previously reported density functional theory study of the (^<i>dm</i>Phebox)Ir complex in alkane C–H activation

Acceptorless, Reversible Dehydrogenation and Hydrogenation of <i>N</i>‑Heterocycles with a Cobalt Pincer Catalyst

Acceptorless, reversible dehydrogenation and hydrogenation reactions involving <i>N</i>-heterocycles are reported with a well-defined cobalt complex supported by an aminobis­(phosphine) [PN­(H)­P] pincer ligand. Several <i>N</i>-heterocycle substrates have been evaluated under dehydrogenation and hydrogenation conditions. The cobalt-catalyzed amine dehydrogenation step, a key step in the dehydrogenation process, has been independently verified. Control studies with related cycloalkanes suggest that a direct acceptorless alkane dehydrogenation pathway is unlikely. The metal–ligand cooperativity is probed with the related [PN­(Me)­P] derivative of the cobalt catalyst. These results suggest a bifunctional dehydrogenation pathway and a nonbifunctional hydrogenation mechanism

Data_Sheet_1_Evaluating occupational exposures of dental nurses: A retrospective study.PDF

ObjectiveThe objective of this study was to investigate occupational blood-borne pathogen exposure among dental nurses and their attitudes toward infected patients, as well as the effectiveness of the training course, to provide a scientific basis for improving the quality of safety management in the dental hospital.Materials and methodsThe study was conducted using questionnaires administered from November 2019 to December 2019 in three hospitals in Sichuan Province, China. Frequencies for answers were calculated and presented as percentages.ResultsIn total, 257 valid questionnaires were returned. Most (61.9%) nurses stated that they were involved in occupational exposure. Among them, 154 had experienced sharp injuries, and the syringe needle was the most common instrument for injuries (45.8%). Twenty-two individuals had mucosal exposure, and the proportion of eye exposure was the highest (90.9%). Only associations between training and mucosal membrane exposure were found; however, the relevance was weak (r = 0.141). Of the participants, 86.4% felt morally responsible for taking care of patients with infectious diseases, and most (92.6%) said they would continue with this career.ConclusionOccupational exposure, particularly to sharp injuries, was common in medical care among dental nurses; however, vocational training had little effect on their incidence. As dental nurses still have positive attitudes toward patients with infectious diseases, more effective training should be conducted.</p

Iron-Catalyzed Homogeneous Hydrogenation of Alkenes under Mild Conditions by a Stepwise, Bifunctional Mechanism

Hydrogenation of alkenes containing polarized CC double bonds has been achieved with iron-based homogeneous catalysts bearing a bis­(phosphino)­amine pincer ligand. Under standard catalytic conditions (5 mol % of (PNHP^iPr)­Fe­(H)₂(CO) (PNHP^iPr = NH­(CH₂CH₂P<i>i</i>Pr₂)₂), 23 °C, 1 atm of H₂), styrene derivatives containing electron-withdrawing para substituents reacted much more quickly than both the parent styrene and substituted styrenes with an electron-donating group. Selective hydrogenation of CC double bonds occurs in the presence of other reducible functionalities such as −CO₂Me, −CN, and N-heterocycles. For the α,β-unsaturated ketone benzalacetone, both CC and CO bonds have been reduced in the final product, but NMR analysis at the initial stage of catalysis demonstrates that the CO bond is reduced much more rapidly than the CC bond. Although Hanson and co-workers have proposed a nonbifunctional alkene hydrogenation mechanism for related nickel and cobalt catalysts, the iron system described here operates via a stepwise metal–ligand cooperative pathway of Fe–H hydride transfer, resulting in an ionic intermediate, followed by N–H proton transfer from the pincer ligand to form the hydrogenated product. Experimental and computational studies indicate that the polarization of the CC bond is imperative for hydrogenation with this iron catalyst

Presentation_2_QTL Mapping of Fiber-Related Traits Based on a High-Density Genetic Map in Flax (Linum usitatissimum L.).ZIP

<p>A genetic map is an important and valuable tool for quantitative trait locus (QTL) mapping, marker-assisted selection (MAS)-based breeding, and reference-assisted chromosome assembly. In this study, 112 F₂ plants from a cross between Linum usitatissimum L. “DIANE” and “NY17” and parent plants were subjected to high-throughput sequencing and specific-locus amplified fragment (SLAF) library construction. After preprocessing, 61.64 Gb of raw data containing 253.71 Mb paired-end reads, each 101 bp in length, were obtained. A total of 192,797 SLAFs were identified, of which 23,115 were polymorphic, with a polymorphism rate of 11.99%. Finally, 2,339 SLAFs were organized into a linkage map consisting of 15 linkage groups (LGs). The total length of the genetic map was 1483.25 centimorgans (cM) and the average distance between adjacent markers was 0.63 cM. Combined with flax chromosome-scale pseudomolecules, 12 QTLs associating with 6 flax fiber-related traits were mapped on the chromosomal scaffolds. This high-density genetic map of flax should serve as a foundation for flax fine QTL mapping, draft genome assembly, and MAS-guided breeding. Ultimately, the genomic regions identified in this research could potentially be valuable for improving flax fiber cultivars, as well as for identification of candidate genes involved in flax fiber formation processes.</p>Significance statement<p>A high-density genetic map of flax was constructed, and QTLs were identified on the sequence scaffolds to be interrelated with fiber-related traits. The results of this study will not only provide a platform for gene/QTL fine mapping, map-based gene isolation, and molecular breeding for flax, but also provide a reference to help position sequence scaffolds on the physical map and assist in the process of assembling the flax genome sequence.</p

Identification and Analysis of Flax Resistance Genes to <i>Septoria linicola</i> (Speg.) Garassini

In this study, the flax (Linum usitatissimum L.) genes associated with resistance to the disease pasmo (Septoria linicola (Speg.) Garassini) were mapped using genome resequencing and bulked segregant analysis (BSA) of genomic DNA of the pasmo-resistant parent y62–9, pasmo-susceptible parent y64–5, and F2 generation segregants. Pasmo resistance genes were identified using Gene Ontology (GO) functional prediction and gene annotation methods. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed the biological information and molecular mechanisms associated with the flax-pathogen interaction. The results of a quantitative real-time PCR analysis revealed that the levels of expression of the three genes Lus10003106, Lus10022077, and Lus10021999 differed between the y62–9 (pasmo-resistant) and y64–5 (pasmo-susceptible) parental flax lines after the inoculation of plants with the pasmo pathogen. Thus, these genes may play key roles in the resistance of flax to pasmo. The results of this study provide a foundation to support future studies of the pathogenesis of flax disease and the discovery and cloning of resistance genes and development of new molecular markers toward the development of pasmo-resistant flax varieties.</p

Presentation_1_QTL Mapping of Fiber-Related Traits Based on a High-Density Genetic Map in Flax (Linum usitatissimum L.).ZIP

<p>A genetic map is an important and valuable tool for quantitative trait locus (QTL) mapping, marker-assisted selection (MAS)-based breeding, and reference-assisted chromosome assembly. In this study, 112 F₂ plants from a cross between Linum usitatissimum L. “DIANE” and “NY17” and parent plants were subjected to high-throughput sequencing and specific-locus amplified fragment (SLAF) library construction. After preprocessing, 61.64 Gb of raw data containing 253.71 Mb paired-end reads, each 101 bp in length, were obtained. A total of 192,797 SLAFs were identified, of which 23,115 were polymorphic, with a polymorphism rate of 11.99%. Finally, 2,339 SLAFs were organized into a linkage map consisting of 15 linkage groups (LGs). The total length of the genetic map was 1483.25 centimorgans (cM) and the average distance between adjacent markers was 0.63 cM. Combined with flax chromosome-scale pseudomolecules, 12 QTLs associating with 6 flax fiber-related traits were mapped on the chromosomal scaffolds. This high-density genetic map of flax should serve as a foundation for flax fine QTL mapping, draft genome assembly, and MAS-guided breeding. Ultimately, the genomic regions identified in this research could potentially be valuable for improving flax fiber cultivars, as well as for identification of candidate genes involved in flax fiber formation processes.</p>Significance statement<p>A high-density genetic map of flax was constructed, and QTLs were identified on the sequence scaffolds to be interrelated with fiber-related traits. The results of this study will not only provide a platform for gene/QTL fine mapping, map-based gene isolation, and molecular breeding for flax, but also provide a reference to help position sequence scaffolds on the physical map and assist in the process of assembling the flax genome sequence.</p

Table_2_QTL Mapping of Fiber-Related Traits Based on a High-Density Genetic Map in Flax (Linum usitatissimum L.).XLSX

<p>A genetic map is an important and valuable tool for quantitative trait locus (QTL) mapping, marker-assisted selection (MAS)-based breeding, and reference-assisted chromosome assembly. In this study, 112 F₂ plants from a cross between Linum usitatissimum L. “DIANE” and “NY17” and parent plants were subjected to high-throughput sequencing and specific-locus amplified fragment (SLAF) library construction. After preprocessing, 61.64 Gb of raw data containing 253.71 Mb paired-end reads, each 101 bp in length, were obtained. A total of 192,797 SLAFs were identified, of which 23,115 were polymorphic, with a polymorphism rate of 11.99%. Finally, 2,339 SLAFs were organized into a linkage map consisting of 15 linkage groups (LGs). The total length of the genetic map was 1483.25 centimorgans (cM) and the average distance between adjacent markers was 0.63 cM. Combined with flax chromosome-scale pseudomolecules, 12 QTLs associating with 6 flax fiber-related traits were mapped on the chromosomal scaffolds. This high-density genetic map of flax should serve as a foundation for flax fine QTL mapping, draft genome assembly, and MAS-guided breeding. Ultimately, the genomic regions identified in this research could potentially be valuable for improving flax fiber cultivars, as well as for identification of candidate genes involved in flax fiber formation processes.</p>Significance statement<p>A high-density genetic map of flax was constructed, and QTLs were identified on the sequence scaffolds to be interrelated with fiber-related traits. The results of this study will not only provide a platform for gene/QTL fine mapping, map-based gene isolation, and molecular breeding for flax, but also provide a reference to help position sequence scaffolds on the physical map and assist in the process of assembling the flax genome sequence.</p